Abstract
Bean leaf abscission (organ separation) correlates with thede novo accumulation of a pI 9.5 cellulase and its mRNA. Overlapping genomic clones encoding the bean abscission cellulase (BAC) were isolated and partially sequenced. In addition, a genomic clone for a soybean abscission cellulase (SAC) was identified and the sequence compared to the BAC genomic sequence. Two 5′-upstream regions are particularly well conserved in the two sequences. Of special interest here is the region between −1 and −200 in the BAC promoter which is highly conserved in the SAC gene. Particle gun bombardment with a BAC promoter construct containing 210 bp of BAC sequence 5′ to the transcription start site was sufficient to drive abscission-specific and ethylene and auxin-regulated transient expression in bean. In addition to the transient expression assay, expression was examined in stably transformed tomato. A similar −210 bp BAC promoter construct supported a low level of ethylene-inducible reporter gene expression in tomato leaf abscission zones and adjacent petioles but not in ethylene-treated stem tissue or fruit. Expression from the −210 promoter in tomato abscission zones was inhibited by silver thiosulfate, an ethylene action inhibitor, and was partially inhibited by treatment with auxin.
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The first two authors (S.M.K. and G.L.M.) share equally in the credit and responsibility for the work presented in this article.
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Koehler, S.M., Matters, G.L., Nath, P. et al. The gene promoter for a bean abscission cellulase is ethylene-induced in transgenic tomato and shows high sequence conservation with a soybean abscission cellulase. Plant Mol Biol 31, 595–606 (1996). https://doi.org/10.1007/BF00042232
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DOI: https://doi.org/10.1007/BF00042232