Abstract
Callus cultures were established from immature embryos of Calotropis gigantea (Linn.) R. Br. on a modified basal medium of Murashige & Skoog supplemented with 1 mgl-1 2,4-D. In addition to 0.1 mgl-1 of NAA the optimal BAP concentration for promoting shoot bud formation and growth was 2 mgl-1. Rooting was induced when shoots were transferred to auxin-supplemented Bonner's solution or half-strength MS basal salt solutions.
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Abbreviations
- NAA:
-
α-naphthaleneacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- IBA:
-
indole-butyric acid
- BAP:
-
6-benzylaminopurine
- Kin:
-
kinetin
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Roy, A.T., De, D.N. Tissue culture and plant regeneration from immature embryo explants of Calotropis gigantea (Linn.) R. Br.. Plant Cell Tiss Organ Cult 20, 229–233 (1990). https://doi.org/10.1007/BF00041886
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DOI: https://doi.org/10.1007/BF00041886