Abstract
Leaf disks from peppermint, spearmint, orange mint, lavender mint and Scotch spearmint were cultured on various Murashige-Skoog-based media in order to regenerate shoots. A significantly larger average number of orange mint leaf disks regenerated shoots on basal medium containing 44.4 μM benzyladenine (BA) and 250 ml l-1 coconut water (CW). Shoots regenerated from peppermint leaf disks cultured on basal medium containing 44.4 μM BA and 250 ml or 450 ml l-1 CW. The most shoots regenerated from orange mint leaf disks cultured on medium containing 10 g l-1 washed Difco Bacto-agar. Disks excised from the bases of the first expanding pair of orange mint leaves cultured under dark conditions regenerated a significantly larger average number of shoots. Histological studies suggested that shoots regenerated from the palisade parenchyma cells associated with vascular tissue.
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Abbreviations
- BA:
-
benzyladenine
- NAA:
-
1-naphthaleneacetic acid
- TIBA:
-
2,3,5-triiodobenzoic acid
- CW:
-
coconut water
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Van Eck, J.M., Kitto, S.L. Regeneration of peppermint and orange mint from leaf disks. Plant Cell Tiss Organ Cult 30, 41–49 (1992). https://doi.org/10.1007/BF00039999
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DOI: https://doi.org/10.1007/BF00039999