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Nuclear protein 780BP from cauliflower binds an element in the 780 gene promoter of T-DNA

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Abstract

A 16 bp site of protein binding has been identified in the promoter of the 780 gene of T-DNA. Specific DNA-protein interactions were demonstrated between a double-stranded oligonucleotide containing this element (5′-TTGAAAAATCAACGCT-3′) and a protein isolated from nuclear extracts of cauliflower inflorescences. Specific bases required for this binding activity (780 binding protein; 780BP) were defined by kinetic competition studies with mutated oligonucleotides, methylation interference assays and DNAse I footprinting. 780BP binding was not competed with up to 1000-fold excess of previously characterized plant regulatory elements such as as-1, the LRE, and the ocs, G-box, and AT-rich elements. In addition, 780BP was shown to bind sequences overlapping a mammalian hormone receptor element with greater affinity than the 780 element.

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References

  1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K: Current Protocols in Molecular Biology. John Wiley, New York (1990).

    Google Scholar 

  2. Banerji J, Rusconi S, Schaffner W: Expression of a β-globin gene is enhanced by remote SV40 DNA sequences. Cell 27: 299–308 (1981).

    Google Scholar 

  3. Beato M: Gene regulation by steroid hormones. Cell 56: 335–344 (1989).

    Google Scholar 

  4. Benfey PN, Takatsuji H, Ren R, Shah DM, Chua N-H: Sequence requirements of the 5-enolpyruvylshikimate-3-phosphate synthase 5′-upstream region for tissue specific expression in flowers and seedlings. Plant Cell 2: 849–856 (1990).

    Google Scholar 

  5. Benoist C, Chambon P: In vivo sequence requirement of the SV40 early promoter region. Nature 290: 304–310 (1981).

    Google Scholar 

  6. Bevan MW, Chilton M-D: T-DNA of the Agrobacterium Ti and Ri plasmids. Annu Rev Genet 16: 357–384 (1982).

    Google Scholar 

  7. Bruce WB, Gurley WB: Functional domains of a T-DNA promoter active in crown gall tumors. Mol Cell Biol 7: 59–67 (1987).

    Google Scholar 

  8. Bruce WB, Bandyopadhyay R, Gurley WB: An enhancer-like element present in the promoter of a T-DNA gene from the Ti plasmid of Agrobacterium tumefaciens. Proc Natl Acad Sci USA 85: 4310–4314 (1988).

    Google Scholar 

  9. Brunt SA, Riehl R, Silver JC: Steroid hormone regulation of the Achlya ambisexualis 85-kilodalton heat shock protein, a component of the Achlya steroid receptor complex. Mol Cell Biol 10: 273–281 (1990).

    Google Scholar 

  10. Chilton M-D, Drummond MH, Merlo DJ, Sciaky D, Montoya AL, Gordon MP, Nester EW: Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11: 263–271 (1977).

    Google Scholar 

  11. Cooney AJ, Tsai SY, O'Malley BW, Tsai M-J: Chicken ovalbumin upstream promoter transcription factor (COUP-TF) dimers bind to different GGTCA response elements, allowing COUP-TF to repress hormonal induction of the vitamin D3, thyroid hormone, and retinoic aicd receptors. Mol Cell Biol 12: 4153–4163 (1992).

    Google Scholar 

  12. Czarnecka E, Key JL, Gurley WB: Regulatory domains of the Gmhsp 17.5E heat shock promoter of soybean. Mol Cell Biol 9: 3457–3463 (1989).

    Google Scholar 

  13. Ellis JG, Llewellyn DJ, Walker JC, Dennis ES, Peacock WJ: The ocs element: a 16 base pair palindrome essential for activity of the octopine synthase enhancer. EMBO J 6: 3202–3208 (1987).

    Google Scholar 

  14. Evans RM: The steroid and thyroid hormone receptor superfamily. Science 240: 889–895 (1988).

    Google Scholar 

  15. Galas DJ, Schmitz A: DNase footprinting: a simple method for the detection of protein-DNA binding specificity. Nucl Acids Res 5: 3157–3170 (1988).

    Google Scholar 

  16. Geuns JMC: Steroid hormones and plant growth and development. Phytochemistry 17: 1–14 (1978).

    Google Scholar 

  17. Green PJ, Kay SA, Chua N-H: Sequence-specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene. EMBO J 6: 2543–2549 (1987).

    Google Scholar 

  18. Green S, Chambon P: Nuclear receptors enhance our understanding of transcription regulation. Trends Genet 4: 309–314 (1988).

    Google Scholar 

  19. Grove MD, Spencer GF, Rohwedder WK, Mandava N, Worley JF, Warthen JD, Steffens GL, Flippen-Anderson JL, Cook JCJr.: A unique plant growth-promoting steroid from Brassica napus pollen. Nature 281: 216–217 (1979).

    Google Scholar 

  20. Gruencer R, Pfitzner UM: The upstream region of the gene for the pathogenesis-related protein from tobacco responds to environmental as well as to developmental signals in transgenic plants. Eur J Biochem 220: 247–255 (1994).

    Google Scholar 

  21. Guiliano G, Pichersky E, Malik VS, Timko MP, Scolnick PA, Cashmore AR: An evolutionary conserved protein binding sequence upstream of a plant light-regulated gene. Proc Natl Acad Sci USA 85: 7089–7093 (1988).

    Google Scholar 

  22. Heftmann E: Functions of steroids in plants. Progr Phytochem 4: 257–276 (1977).

    Google Scholar 

  23. Hewitt FR, Hough T, O'Neill P, Sasse JM, Williams EG, Rowan KS: Effect of brassinolide and other growth regulators on the germination and growth of pollen tubes of Prunus avium using a multiple hanging-drop assay. Aust J Plant Physiol 12: 201–211 (1985).

    Google Scholar 

  24. Hollenberg SM, Weinberger C, Ong ES, Cerelli G, Oro A, Lebo R, Thompson EB, Rosenfeld MG, Evans RM: Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature 318: 635–614 (1985).

    Google Scholar 

  25. Jofuku KD, Okamuro JK, Goldberg RB: Interaction of an embryo DNA binding protein with a soybean lectin gene upstream region. Nature 328: 734–737 (1987).

    Google Scholar 

  26. Kadonaga JT, Tjian R: Affinity purification of sequence-specific DNA binding proteins. Proc Natl Acad Sci USA 83: 5889–5893 (1986).

    Google Scholar 

  27. Kadonaga JT: Purification of sequence-specific binding proteins by affinity chromatography. Methods Enzymol 208: 10–45 (1991).

    Google Scholar 

  28. Karcher SJ, DiRita VJ, Gelvin SB: Transcript analysis of TR DNA in octopine-type crown gall tumors. Mol Gen Genet 194: 159–165 (1984).

    Google Scholar 

  29. Laemmli UK: Cleavage of Structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685 (1970).

    Google Scholar 

  30. Laudet V, Hanni C, Coll J, Catzeflis F, Stehelin D: Evolution of the nuclear receptor gene superfamily. EMBO J 11: 1003–1013 (1992).

    Google Scholar 

  31. Lin S-Y, Riggs AD: Photochemical attachment of lac repressor to bromodeoxyuridine substituted lac operator by ultraviolet radiation. Proc Nat Acad Sci USA 71: 947–951 (1974).

    Google Scholar 

  32. Mandava NB: Plant growth-promoting brassinosteroids. Annu Rev Plant Physiol Plant Mol Biol 39: 23–52 (1988).

    Google Scholar 

  33. Maniatis T, Goodbourn S, Fischer JA: Regulation of inducible and tissue specific gene expression. Science 230: 1237–1244 (1987).

    Google Scholar 

  34. Maxam A, Gilbert W: Sequencing end-labeled DNA with base-specific chemical cleavages. Meth Enzymol 65: 499–560 (1980).

    Google Scholar 

  35. McMorris TC: Sex hormones of the aquatic fungus Achlya. Lipids 13: 716–722 (1970).

    Google Scholar 

  36. Mitchell JW, Mandava N, Worley JF, Plimmer JR, Smith MV: Brassins: a new family of plant hormones from rape pollen. Nature 225: 1065–1066 (1970).

    Google Scholar 

  37. Moore D: Diversity and unity in the nuclear hormone receptors: a terpenoid receptor superfamily. New Biol 2: 100–105 (1990).

    Google Scholar 

  38. Nauber U, Pankratz MJ, Kienlin A, Seifert E, Klemm U, Jackle H: Abdominal segmentation of the Drosophila embryo requires a receptor-like protein encoded by the gap gene knirps: Nature 336: 489–492 (1988).

    Google Scholar 

  39. Nester EW, Gordon MP, Amasino RM, Yanofsky MF: Crown gall: a molecular and physiological analysis. Annu Rev Plant Physiol 35: 387–413 (1984).

    Google Scholar 

  40. O'Grady K: Site directed mutagenesis of the 780 enhancer-like element. Ph. D. dissertation, University of Florida (1993).

  41. Oro AE, Ong ES, Margolis JW, Posakony JW, McKeon M, Evans RM: The Drosophila gene knirps-related is a member of the steroid receptor superfamily. Nature 336: 493–496 (1988).

    Google Scholar 

  42. Rubin CM, Schmid CW: Pyrimidine-specific chemical reactions useful for DNA sequencing. Nucl Acids Res 8: 4613–6419 (1980).

    Google Scholar 

  43. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).

    Google Scholar 

  44. Segraves WA, Hogness DS: The E75 ecdysone-inducible gene responsible for the 75B early puff in Drosophila encodes two new members of the steroid receptor superfamily. Genes Devel 4: 204–219 (1990).

    Google Scholar 

  45. Singh H, Sen R, Baltimore D, Sharp PA: A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Nature 319: 154–158 (1986).

    Google Scholar 

  46. Singh K, Tokuhisa JG, Dennis ES, Peacock WJ: Saturation mutagenesis of the octopine synthase enhancer: correlation of mutant phenotypes with binding of a nuclear protein factor. Proc Natl Acad Sci USA 86: 3733–3737 (1989).

    Google Scholar 

  47. Singh K, Dennis E, Ellis J, Llewellyn DJ, Tokuhisa JG, Wahleithner JA, Peacock WJ: OCSBF-1, a maize ocs enhancer binding factor: isolation and expression during development. Plant Cell 2: 891–903 (1990).

    Google Scholar 

  48. Sladek FM, Zhong W, Lai E, DarnellJr JE: Liver-enriched transcription factor HNF-4 is a novel member of the steroid hormone receptor superfamily. Genes Devel 4: 2353–2365 (1990).

    Google Scholar 

  49. Slama K: Animal hormones and antihormones in plants. Biochem Physiol Pflanzen 175: 177–193 (1980).

    Google Scholar 

  50. Smith PM, Taylor PE, Sasse JM, Yokta T: Towards a brassinosteroid receptor. Annu Proc Plant Gro Reg Soc Am 19: 93–97 (1992).

    Google Scholar 

  51. Takatsuji H, Mori M, Benfey PN, Ren L, Chua NH: Characterization of a zinc-finger DNA-binding protein expressed specifically in petunia petals and seedlings. EMBO J 11: 241–249 (1992).

    Google Scholar 

  52. Thompson CC, Weinberger C, Lebo R, Evans RM: Identification of a novel thyroid hormone receptor expressed in mammalian central nervous system. Science 237: 1610–1614 (1987).

    Google Scholar 

  53. Thompson GA, Siemieniak DR, Sieu LC, Slighton JL, Larkins BA: Sequence analysis of linked maize 22 kDa alpha-zein genes. Plant Mol Biol 18: 827–833 (1992).

    Google Scholar 

  54. Tokuhisa JG, Singh K, Dennis ES, Peacock WJ: A DNA-binding protein factor recognizes two binding domains within the octopine synthase enhancer. Plant Cell 2: 215–224 (1990).

    Google Scholar 

  55. Truss M, Chalepakis G, Beato M: Contacts between steroid hormone receptors and thymines in DNA: an interference method. Proc Natl Acad Sci USA 87: 7180–7184 (1990).

    Google Scholar 

  56. Wang L-H, Tsai SY, Cook RG, Beattie WG, Tsai M-J, O'Malley BW: COUP transcription factor is a member of the steroid receptor superfamily. Nature 340: 163–168 (1989).

    Google Scholar 

  57. Weising K, Schell J, Kahl G: Foreign genes in plants: transfer, structure, expression, and applications. Annu Rev Genet 22: 421–477 (1988).

    Google Scholar 

  58. Winter JA, Wright RL, Gurley WB: Map locations of five transcripts'homologous to Tr-DNA in tobacco and sunflower crown gall tumors. Nucl Acids Res 12: 2391–2406 (1984).

    Google Scholar 

  59. Wu C, Wilson S, Walker B, Dawid I, Paisley T, Zimarino V, Ueda H: Purification and properties of Drosophila heat shock activator protein. Science 238: 1247–1252 (1987).

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology and Cell Science, University of Florida, 32611, Gainesville, FL, USA

    Eloise C. Adams & William B. Gurley

  2. Plant Molecular and Cellular Biology Program, University of Florida, 32611, Gainesville, FL, USA

    Eloise C. Adams & William B. Gurley

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  1. Eloise C. Adams
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Adams, E.C., Gurley, W.B. Nuclear protein 780BP from cauliflower binds an element in the 780 gene promoter of T-DNA. Plant Mol Biol 26, 377–392 (1994). https://doi.org/10.1007/BF00039547

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  • DOI: https://doi.org/10.1007/BF00039547

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