Summary
The purpose of this research was, firstly to determine the ability of grapevine in vitro cultures to synthesize resveratrol, a stilbene-type phytoalexin that is considered to be a good marker for resistance of grapevines to Botrytis cinerea, the causal organism for grey mould. Secondly, this study sought to establish the relationship between phytoalexin production potential and resistance to Botrytis cinerea in grapevines. In this aim, resveratrol production was assessed in 13 Vitis species or cultivars. A good correlation appeared between resveratrol production by grapevine in vitro cultures and grey mould resistance except for two Vitis spp. for which no correlation was observed, thus suggesting that resistance of grapevines may sometimes be associated with factors other than stilbene phytoalexins. In view of the results obtained, the potential use of resveratrol induction and of in vitro methods as a tool for screening grapevines for resistance to B. cinerea was discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barbier, M. & R. Bessis, 1990. Isolation and culture of grapevine leaf protoplasts (cv. Chardonnay). Euphytica 47: 39–44.
Barlass, M., R.M. Miller & A.J. Antcliff, 1986. Development of methods for screening grapevines for resistance to downy mildew. I. Dual culture in vitro. Am. J. Enol. Vitic. 37: 61–66.
Barlass, M., R.M. Miller & T.J. Douglas, 1987. Development of methods for screening grapevines for resistance to downy mildew. II. Resveratrol production. Am. J. Enol. Vitic. 38: 65–68.
Bessis, R., 1986. Grapevine physiology: the contribution of culture in vitro. Experientia 42: 927–933.
Bessis, R. & C. Labroche, 1985. Callogenèse et régénération chez le Pinot, le Chardonnay et le Gamay. p. 127–134. In: Möet (Ed). Colloque Amélioration de la vigne et culture in vitro. Möet-Hennessy, 071 Paris.
Bouquet, A., 1989. Intérêt des techniques de culture in vitro pour l'amélioration génétique de la vigne. Bulletin de l'OIV 62: 179–192.
Calderon, A.A., J.M. Zapata, R. Munoz, M.A. Pedreno & A. Ros-Barcelo, 1993. Resveratrol production as a part of the hypersensitive-like response of grapevine cells to an elicitor from Trichoderma viride. New Phytol. 124: 455–463.
Day, G.H., G. Mur & D. Boubals, 1993. A new disease reaction type in dual culture of Plasmopara viticola and in vitro plantlets of grapevines (Vitis spp.). p. 459. In: B. Fritig & M. Legrand (Eds). Mechanisms of Plant Defense Responses. Kluwer Academic Publishers, Dordrecht.
Dercks, W. & L.L. Creasy, 1989. The significance of stilbene phytoalexins in the Plasmopara viticola-grapevine interaction. Physiol. Molec. Plant Pathol. 34: 189–202.
Fournioux, J.C. & R. Bessis, 1993. Use of carbon dioxide enrichment to obtain adult morphology of grapevine in vitro. Plant Cell Tissue and Organ Culture 33: 51–57.
Galet, P., 1977. Les maladies et parasites de la vigne. Imprimerie du paysan du midi, Montpellier.
Grenan, S., 1982. Implications fondamentales et appliquées de la culture in vitro de Vitis vinifera. Thèse Doc. Sci. Université de Paris, Orsay.
Hain, R., B. Bieseler, H Kindl, G. Schröder, 1990. Expression of a stilbene synthase gene in Nicotiana tabacum results in synthesis of the phytoalexin resveratrol. Plant Molec. Biol. 15: 325–335.
Hain, R., H.J. Reif, E. Krause, R. Langebartels, H. Kindl, B. Vornam, W. Wiese, E. Schmelzer, P. Schreier, R. Stöcker & K. Stenzel, 1993. Disease resistance results from foreign phytoalexin expression in a novel plant. Nature 361: 153–156.
Hoos, G. & R. Blaich, 1988. Metabolism of stilbene phytoalexins in grapevines: oxidation of resveratrol in single cell cultures. Vitis 27: 1–12.
Jeandet, P., R. Bessis & B. Gautheron, 1991. The production of resveratrol (3,5,4′-trihydroxystilbene) by grapes berries in different developmental stages. Am. J. Enol. Vitic. 42: 41–46.
Jeandet, P., R. Bessis, B.F. Maume, P. Meunier, D. Peyron & P. Trollat, 1995a. Effect of enological practices on the resveratrol isomer content of wine. J. Agric. Food Chem. 43: 316–319.
Jeandet, P., R. Bessis, M. Sbaghi & P. Meunier, 1995b. Production of the phytoalexin resveratrol by grape as a response to Botrytis attack under natural conditions. J. Phytopathol. 143: 135–139.
Jeandet, P., M. Sbaghi & R. Bessis, 1992. The use of phytoalexin induction and of in vitro methods as a tool for screening grapevines for resistance to Botrytis cinerea. p. 109–112. In: K. Verhoeff, N.E. Malathrakis & B. Williamson (Eds). Recent Advances in Botrytis Research. Pudoc Scientific Publishers, Wageningen.
Klempka, K.C., C.P. Meredith & M.A. Sall, 1984. Dual culture of grape powdery mildew (Uncinula necator Burr.) on its host (Vitis vinifera L.). Am. J. Enol. Vitic. 35: 170–174.
Langcake, P., 1981. Disease resistance of Vitis spp. and the production of the stress metabolites resveratrol, epsilon-vinifern, alphaviniferin and pterostilbene. Physiol. Plant Pathol. 18: 213–226.
Langcake, P. & W.V. McCarthy, 1979. The relationship of resveratrol production to infection of grapevine leaves by Botrytis cinerea. Vitis 18: 224–253.
Langcake, P. & R.J. Pryce, 1977. The production of resveratrol and the viniferins by grapevines in response to ultraviolet irradiations. Phytochemistry 16: 1193–1196.
Lee, T.C. & T. Wicks, 1982. Dual culture of Plasmopara viticola and grapevine and its application to systemic fungicide evaluation. Plant Dis. 66: 308–310.
Liswidowati, M.F., F. Melchior, F. Hohmann, B. Schwer & H. Kindl, 1991. Induction of stilbene synthase by Botrytis cinerea in cultured grapevine cells. Planta 183: 307–314.
Mullins, M.G., 1985. Amplification of clonal variation in the grapevine: progress and prospects. p. 63–73. In: Moët (Ed). Colloque 071 Amélioration de la vigne et culture in vitro. Moët-Hennessy, Paris.
Mullins, M.G., A. Bouquet & L.E. Williams, 1992. Biology of the grapevine. Cambridge University Press, Cambridge.
Mullins, M.G. & C. Srinivasan, 1976. Somatic embryos and plantlets from an ancient clone of the grapevine (cv. Cabernet sauvignon) by apomixis in vitro. J. Exp. Bot. 27: 1022–1030.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plant 15: 473–496.
Pool, R.M., L.L. Creasy & A.S. Frackelton, 1981. Resveratrol and the viniferins, their application to screening for disease resistance in grape breeding programs. Vitis 20: 136–145.
Rajasekaran, K. & M.G. Mullins, 1983. The origin of embryos and plantlets from cultured anthers of hybrid grapevines. Am. J. Enol. Vitic. 34: 108–113.
Schöppner, A. & H. Kindl, 1984. Purification and properties of a stilbene synthase from induced cell suspension cultures of peanut. J. Biol. Chem. 259: 6806–6811.
Skene, K.G.M., 1975. Production of calus from protoplasts of cultured grape pericarp. Vitis 14: 177–180.
Soulié, O., J.P. Roustan & J. Fallot, 1993. Early in vitro selection of eutypine-tolerant plantlets. Application to screening of Vitis vinifera cv. Ugni blanc somaclones. Vitis 32: 243–244.
Stein, U. & R. Blaich, 1985. Untersuchungenüber stilbenproduktion und Botrytis-anfälligkeit bei Vitis arten. Vitis 24: 75–87.
Vail, M.E. & J.J. Marois, 1991. Grape cluster architecture and the susceptibility of berries to Botrytis cinerea. Phytopathology 81: 188–191.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sbaghi, M., Jeandet, P., Faivre, B. et al. Development of methods using phytoalexin (resveratrol) assessment as a selection criterion to screen grapevine in vitro cultures for resistance to grey mould (Botrytis cinerea). Euphytica 86, 41–47 (1995). https://doi.org/10.1007/BF00035937
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00035937