Abstract
Cultural and physiological properties, serology, plasmid profiles and infective traits were determined for 23 strains of rhizobia isolated from various Hedysarum species: H. coronarium (common name: sulla) (16), H. carnosum (1), H. alpinum (3), H. mackenzii (2) and H. pallens (1) from Portugal, Spain, Tunisia, Alaska and Israel. Strains isolated from H. alpinum, H. mackenzii and H. pallens have slow growth rates on yeast-extract mannitol medium and were unable to nodulate H. coronarium plants, whereas the latter were effectively nodulated by all sixteen fast growing strains from sulla. Regardless of the country of origin all H. coronarium strains fell into one serogroup and were not serologically related with strains of other Hedysarum species. The RAPD (random amplified polymorphic DNA) fingerprinting method which was carried out on five H. coronarium and three H. alpinum strains allowed distinction to be made among serologically related rhizobia. No particular plasmid profile pattern was observed in relation to the host or geographical origin of the strains.
Similar content being viewed by others
References
Allen O N and Allen E K 1981 The Leguminosae. Univ. of Wisconsin Press, Madison, WI, USA. 812 p.
Allen E K, Allen O N and Klebesabel L J 1964 An insight into symbiotic nitrogen-fixing plant association in Alaska. In Alaska Science Conference, Science in Alaska. Anchorage. Alaska. Proceedings 14, 54–63.
Beringer J E 1974 R factor transfer in Rhizobium leguminosarum. J. Gen. Microbiol. 84, 188–198.
Berryhill D L, Shroeder M B and Obermiller T L 1985 Plasmid contents of commercial Rhizobium leguminosarum biovar. phaseoli strains. Research report #105, p 4, Agricultural Experiment Station, North Dakota State University, Fargo, USA.
Casse F, Boucher C, Julliot J S, Michel M and Denarie J 1979 Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis. J. Gen. Microbiol. 113, 229–242.
Eckhardt T 1978 A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid 1, 584–588.
Engvall E and Perlmann P 1971 Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8, 871–874.
Fuhrmann J and Wollum II A G 1985 Simplified enzyme-linked immunosorbent assay for routine identification of Rhizobium japonicum antigens. Appl. Environ. Microbiol. 49, 1010–1013.
Graham P H and Parker C A 1964 Diagnostic features in characterization of the root-nodule bacteria of legumes. Plant and Soil 3, 383–396.
Gurfel D, Lobel R and Schiffmann J 1982 Symbiotic nitrogen-fixing activity and yield potential of inoculated Hedysarum coronarium in Israel. Isr. J. Bot. 31, 296–304.
Hely F W and Ofer I 1972 Nodulation and frequencies of wild leguminous species in the Northern Negev region of Israel. Aust. J. Agric. Res. 23, 267–284.
Hynes M F, Simon R and Puhler A 1985 The development of plasmid-free strains of Agrobacterium tumefaciens by using incompatibility with a Rhizobium meliloti plasmid to eliminate pAtC58. Plasmid 13, 99–105.
Kernick M D 1978 Indigenous arid and semi-arid forage plants of North Africa, the Near and Middle East. In Ecological Management of Arid and Semi-arid Rangelands in Africa and the Near and Middle East, vol IV, pp 599–619. FAO, Rome, Italy.
Kishinevsky B and Gurfel D 1980 Evaluation of enzyme-linked immunosorbent assay (ELISA) for serological identification of different Rhizobium strains. J. Appl. Bacteriol. 49, 514–526.
Kishinevsky B and Maoz A 1983 ELISA identification of Rhizobium strains by use of enzyme labeled protein A. Curr. Microbiol. 2, 45–49.
Kishinevsky B, Maoz A, Gurfel D and Nemas C 1984 A comparative evaluation of direct and indirect enzyme-linked immunosorbent assay (ELISA) in serological studies of Rhizobium strains. In Symbiotic Nitrogen Fixation, Vol 1. Ed. B S Ghai. USG Publishers and Distributors, Ludhiana, India.
Long S R 1992 Genetic analysis of Rhizobium nodulation. In Biological Nitrogen Fixation. Eds. G Stacey, R H Burris and H J Evans. pp 560–597. Chapman and Hall, New York.
Mozo T, Cabrera E and Ruiz-Argueso T 1988 Diversity of plasmid profiles and conservation of symbiotic nitrogen fixation genes in newly isolated Rhizobium strains nodulating sulla (Hedysarum coronarium L.). Appl. Environ. Microbiol. 54, 1262–1267.
Rodriguez-Navarro D N, Temprano F and Orive R 1991 Survival of Rhizobium sp. (Hedysarum coronarium L.) on peat-based inoculants and inoculated seeds. Soil Biol. Biochem. 23, 375–379.
Roizin M B 1959 The root nodules of leguminous plants of the Kola Peninsula, Bot. Zh. 44, 467–474 (In Russian).
Squartini A, Dazzo F B, Casella S and Nuti M P 1993 The its drought-tolerant host Hedysarum coronarium. Symbiosis 15, 227–238.
Stowers M D and Eaglesham A R J 1984 Physiological and symbiotic characteristics of fast-growing Rhizobium japonicum. Plant and Soil 77, 3–14.
Vincent J M 1970 A manual for the practical study of the root nodule bacteria. Blackwell Scientific Publications, Oxford, UK.
Webb K E and Wilson R 1991 Extraction, purification and assay of DNA. In Genetic Manipulation: Techniques and Application. Ed. J Grange, M A Fox and N Morgan. pp 1–14. Technical series, Blackwell Scientific Publications, Oxford, UK.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kishinevsky, B.D., Sen, D. & Yang, G. Diversity of Rhizobia isolated from various Hedysarum species. Plant Soil 186, 21–28 (1996). https://doi.org/10.1007/BF00035051
Issue Date:
DOI: https://doi.org/10.1007/BF00035051