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Hormone independent root organ cultures of rye (Secale cereale)

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Abstract

This work describes the growth of rye root organ cultures which were capable of being repeatedly subcultured in hormone-free medium. They showed morphological characteristics, growth rate, inability to produce shoots, and response to auxins and cytokinins similar to those of the Agrobacterium rhizogenes (Ri plasmid) transformed hairy root cultures of tobacco and red beet which were used for comparison. The root cultures of rye were initiated from callus produced on a medium containing the growth regulators (plant hormones) 2,4-d and kinetin, then transferred to hormone-free medium. However not all rye explants gave rise to callus that would differentiate into stable hairy root cultures and rye seedling root explants did not grow if placed directly on a hormone-free medium. Rice and wheat produced callus and roots on a medium containing hormones but root organ cultures could not be maintained on a hormone-free medium.

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Authors and Affiliations

  1. School of Biological Sciences, University of Surrey, GU2 5XH, Guildford, Surrey, UK

    Philip John Whitney

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  1. Philip John Whitney
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Whitney, P.J. Hormone independent root organ cultures of rye (Secale cereale). Plant Cell Tiss Organ Cult 46, 109–115 (1996). https://doi.org/10.1007/BF00034843

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  • DOI: https://doi.org/10.1007/BF00034843

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