Summary
Two crosses between Rosa hybrida L. cultivars, that fail to produce progenies by conventional means, were carried out. In one of them, total hip abscision occured seven weeks after pollination; in the other one, only 2% of the fertilized hips remained on the plants after eleven weeks. Four- and five-week-old fertilized ovules were isolated in the first cross and six-week-old embryos in the second. The ovules were cultured on six media which differed in their mineral salt and sucrose concentrations while the embryos were cultured on only one of these media and eventually cold treated for one month at 4° C before being placed in a culture room set at 23° C. The embryos that had been isolated in ovulo when they were still not visible under a binocular lens developed atypically. The embryos isolated in ovulo when they were heart-shaped and on average 0.27 mm long performed in ovulo germination on some media and/or enlargement on all of them after two weeks; after another two week culture, once isolated from the ovule, all of them germinated. The cultured isolated embryos, that were exposed to cold, enlarged and germinated more rapidly when placed at 23° C than those not exposed to cold. Furthermore, the plantlets resulting from untreated embryo germination were characterized by large cotyledons which were only partially green. These results are discussed in regard to embryogenesis, precocious germination and dormancy.
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Arene, L., C. Pellegrino & S. Gudin, 1993. A comparison of the somaclonal variation level of Rosa hybrida L. cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues. Euphytica: in press.
Arisumi, T., 1987. Cytology and morphology of ovule culturederived interspecific Impatiens hybrids. J. Am. Soc. Hort. Sci. 112: 1026–1031.
Asen, S. & R.E. Larsen, 1951. Artificial culturing of rose embryos. Pa. Agr. Exp. Sta. Prog. Rep. 40, 4p.
Bajaj, Y.P.S., 1966. Growth of Hyoscyamus niger ovaries in culture. Phyton 23: 57–62.
Bajaj, Y.P.S., P., Kumar, M.M., Singh & K.S., Labana, 1982. Interspecific hybridization in the genus Arachis through embryo culture. Euphytica 31: 365–370.
Bauchan, G.R., 1987. Embryo culture of Medicago scutellata and M. sativa. Plant Cell Tiss. Org. Cult. 10: 21–29.
Bulard, C., 1968. Acquisitions récentes sur la dormance embryonnaire. Bull. Soc. Fr. Physiol. Veg. 14: 11–23.
Bulard, C. & M.T., Lepage-Degivry, 1985. Phytohormones in seed and fruit development. In: M., Bopp (Ed.), Plant Growth Substances. pp. 308–314. Springer-Verlag, Berlin, Heidelberg.
Burger, D.W., L., Liu, K.W., Zary & C.I., Lee, 1990. Organogenesis and plant regeneration from immature embryos of Rosa hybrida L. Plant Cell Tiss. Org. Cult. 21: 147–152.
Chen, L. & T., Adachi, 1992. Embryo abortion and efficient rescue in interspecific hybrids, Lycopersicon esculentum and the ‘peruvianum-complex’. Japan J. Breed. 42: 65–77.
Cohen, D., G., Ladizinsky, M., Ziv & F.J., Muehlbauer, 1984. Rescue of interspecific Lens hybrids by means of embryo culture. Plant Cell Tiss. Org. Cult. 3: 343–347.
Custers, J.B.M., W., Eikelboom, J.H.W., Bergervoet & J.P.van, Eijk, 1992. In ovulo embryo culture of tulip (Tulipa L.); effects of culture conditions on seedling and bulblet formation. Scientia Horticulturae 51: 111–122.
Davidson, O.W., 1934. The germination of non viable peach seeds. Proc. Am. Soc. Hort. Sci. 30: 129–132.
Degivry, M.T., 1966. Revue Bibliographique sur les problèmes liés aux cultures in vitro d'embryons immatures. Bull. Sci. Bourgogne 24: 57–87.
Dudits, D., L., Bögre & J., Györgyey, 1991. Molecular and cellular approaches to the analysis of plant embryo development from somatic cell in vitro. J. Cell. Sci. 99: 475–484.
Gill, M.S. & Y.P.S., Bajaj, 1984. Interspecific hybridization in the genus Gossypium through embryo culture. Euphytica 33: 305–311.
Gray, D.J., L.C., Fischer & J.A., Mortensen, 1987. Comparison of methodologies for in ovulo embryo rescue of seedless grapes. HortSci. 22: 1334–1335.
Gudin, S., L., Arene, A., Chavagnat & C., Bulard, 1990. Influence of endocarp thickness on rose achene germination: genetic and environmental factors. HortSci. 25: 786–788.
Imanishi, S., 1988. Efficient ovule culture for the hybridization of Lycopersicon esculentum and L. peruvianum, L. glandulosum. Japan J. Breed. 38: 1–9.
Inomata, N., 1979. Production of interspecific hybrids in Brassica campestris x B. oleracea by culture in vitro of excised ovaries. Japan J. Breed. 29: 115–120.
Lammerts, W.E., 1946. Use of embryo culture in rose breeding. Plants and Gardens 2: 11.
Lepage-Degivry, M.T., 1970. Dormance de graine associée à une immaturité de l'embryon: étude en culture in vitro chez Magnolia soulangeana et M. grandiflora. Planta 90: 267–271.
McCoy, T.J., 1985. Interspecific hybridization of Medicago sativa L. and M. rupestris M.B. using ovule-embryo culture. Can. J. Genet. Cytol. 27: 238–245.
Morel, G. & C., Martin, 1955. Guérison de pommes de terre atteintes de maladies à virus. C. R. Acad. Sci. Paris 41: 472–475.
Murashige, T. & F., Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15: 473–497.
Norstog, K. & R.M., Klein, 1972. Development of cultured barley embryos. II. Precocious germination and dormancy. Can. J. Bot. 50: 1887–1894.
Pattee, H.E., H.T., Stalker & J.P., Moss, 1988. Embryo rescue in wide crosses in Arachis. 2. Embryo development in cultured peg tips of Arachis hypogaea. Ann. Bot. 61: 103–112.
Pechoutre, F., 1902. Contribution à l'étude du developpement de l'ovule et de la graine des Rosacées. An. Sci. Nat. 16: 60–87.
Petiot, M.T., 1987. Viviparité induite chez quatre lignées de Petunia à semences dormantes par la culture in vitro de jeunes fruits en cours de maturation. C.R. Acad. Sci. Paris 305: 543–548.
Quazi, M.H., 1988. Interspecific hybrids between Brassica napus L. and B. oleracea L. developed by embryo culture. Theor. Appl. Genet. 75: 309–318.
Raghavan, V., 1980. Embryo culture. Int. Rev. Cytol. 11: 209–240.
Srivastava, P.S., A., Varga & J., Bruinsma, 1980. Growth in vitro of fertilized ovules of pea, Pisum sativum L., with and without pods. Z. Pflanzenphysiol. 98: 347–354.
Thevenot, C. & M., Durand, 1973. Mise en évidence d'une dormance chez les embryons de pommier immatures. C.R. Acad. Agr. Paris 59: 908–918.
Tillberg, E., 1983. Levels of endogenous abscisic acid in achenes of Rosa rugosa during dormancy release and germination. Physiol. Plantarum 58: 243–248.
Tukey, H.B., 1933. Artificial culture of sweet cherry embryos. The Journal of Heredity 24: 7–12.
Tukey, H.B., 1934. Artificial culture methods for isolated embryos of deciduous fruits. Proc. Am. Soc. Hort. Sci. 32: 313–322.
Van, Lammeren, A.A.M., 1988. Observations on the structural development of immature maize embryos (Zea mays L.) during in vitro culture in the presence or absence of 2,4-D. Acta Bot. Neerl. 37: 49–61.
Visser, T., 1954. After ripening and germination of apple seed in relation to the seed coats. Proc. K. Ned. Akad. Wet. C. 57: 175–185.
Williams, E.G. & G.De, Lautour, 1980. The use of embryo culture with transplanted nurse endosperm for the production of interspecific hybrids in pasture legumes. Bot. Gaz. 141: 252–257.
Williams, W.M., G.De, Lautour & E.G., Williams, 1987. A hybrid between Ornithopus sativus and O. compressus cv. Pitman obtained with the aid of ovule-embryo culture. Aust. J. Bot. 35: 219–226.
Zagaja, S.W., 1961. Preliminary results of investigation on the effect of low temperatures on the development of seedlings from immature embryos of sweet and sour cherries. Bull. Acad. Pol. Sci. 3: 113–115.
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Gudin, S. Embryo rescue in Rosa hybrida L.. Euphytica 72, 205–212 (1993). https://doi.org/10.1007/BF00034159
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DOI: https://doi.org/10.1007/BF00034159