Abstract
Root segments obtained from aseptically germinated seedlings of Brassica napus cv. Westar were used to optimize conditions for high-frequency shoot bud differentiation. The presence of low kinetin (0.5 μM) and relatively high indole-butyric acid (1.0 μM) levels facilitated optimum shoot bud differentiation. Modified MS medium (MMS) was superior to the other three basal media tested (MS, B5 and White's). Elevated sodium dihydrogen phosphate levels increased the differentiation of shoot buds. Increasing or decreasing the level of sucrose from 3% reduced the frequency of explants forming shoot buds. Addition of glutamine enhanced both the frequency of responding explants, as well as the number of shoots per responding explant. Root segments from 13-day-old seedlings produced the highest response (58%) in the presence of 100 mg l-1 glutamine. The position of the segment on the main root, size, and the presence or absence of lateral roots altered the morphogenic response. Sealing of the donor seedling cultures with Parafilm® instead of Stretch' n seal® resulted in a higher production of shoot buds, although root segment cultures were not affected by the type of sealing. Spontaneous rooting occurred on all developed shoots.
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Dedicated to Dr. Friedrch Constabel on the occasion of his 60th birthday
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Sharma, K.K., Thorpe, T.A. In vitro regeneration of shoot buds and plantlets from seedling root segments of Brassica napus L.. Plant Cell Tiss Organ Cult 18, 129–141 (1989). https://doi.org/10.1007/BF00033471
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DOI: https://doi.org/10.1007/BF00033471