Abstract
The medium used for the growth of anthocyanin-accumulating wild carrot (D. carota) suspension cultures contained ammonia as a sole nitrogen source and was buffered with succinate. Ammonia was the first nutrient to be completely utilized.
The uptake of carbohydrate, phosphate and succinate continued after ammonia depletion. Biomass accumulation was faster and greater when sucrose was initially present in the medium than when glucose was present. When sucrose was provided in the medium it was rapidly hydrolysed to glucose and fructose and the fructose was used preferentially to glucose. Anthocyanin accumulation was rapid after ammonia fell below 3 mM and until the pH of the medium rose from 4.5 to 5.1 or 5.2.
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Dedicated to Dr. Friedrich Constabel on the occasion of his 60th birthday
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Dougall, D.K., Frazier, G.C. Nutrient utilization during biomass and anthocyanin accumulation in suspension cultures of wild carrot cells. Plant Cell Tiss Organ Cult 18, 95–104 (1989). https://doi.org/10.1007/BF00033468
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DOI: https://doi.org/10.1007/BF00033468