Summary
We describe in this paper the construction and use of a set of novel Ti plasmid-derived vectors that can be used to produce transgenic plants. These vectors are based on one of two strategies: 1) double recombination into the wild-type Ti plasmid of genetic information flanked by two T-DNA fragments on a wide-host range plasmid; 2) the binary vector strategy. The vector based on the double recombination principle contains a kanamycin resistance gene for use as a plant selectable marker, a polylinker for the insertion of foreign genes, and a nopaline synthase gene. The vector was constructed such that a disarmed T-DNA results from the double recombination event. The binary vector combines several advantageous features including an origin of replication that is stable in Agrobacterium in the absence of selection, six unique sites for insertion of foreign genes, an intact nopaline synthase gene, and a kanamycin resistance marker for selection of transformed plant cells. All of these vectors have been used to produce tobacco plants transformed with a variety of foreign genes.
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References
An G, Watson B, Stachel S, Gordon M, Nester E: New cloning vehicles for transformation of higher plants. EMBO J 4:277–284, 1985.
Bagdasarian M, Amann E, Lurz R, Rückert B, Bagdasarian M: Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled expression vectors. Gene 26:273–282, 1983.
Bagdasarian M, Timmis K: Host: vector systems for gene cloning in Pseudomonas. Curr Top Microbiol Immunol 96:47–67, 1982.
Barta A, Sommergruber K, Thompson D, Hartmuth K, Matzke M, Matzke A: The expression of a nopaline synthase-human growth hormone chimaeric gene in transformed tobacco and sunflower callus tissue. Plant Molec Biol, 6:347–357, 1986.
Barton K, Binns A, Matzke A, Chilton M-D: Regeneration of intact tobacco plants containing full length copies of genetically engineered T-DNA, and transmission of T-DNA to R1 progeny. Cell 32:1033–1043, 1983.
Beck E, Ludwig G, Auerswald E, Reiss B, Schaller H: Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19:327–336, 1982.
Bevan M, Chilton M-D: T-DNA of the Agrobacterium Ti and Ri plasmids. Ann Rev Genet 16:357–384, 1982.
Bevan M, Flavell R, Chilton M-D: A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation. Nature 304:184–187, 1983.
Bevan M: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12:8711–8721, 1984.
Bräu B, Pilz U, Piepersberg W: Genes for gentamicin-(3)-N-acetyltransferases III and IV: Nucleotide sequence of the AAC(3)-IV gene and possible involvement of an IS140 element in its expression. Mol Gen Genet 193:179–187, 1984.
De Block M, Herrera-Estrella L, Van Montagu M, Schell J, Zambryski P: Expression of foreign genes in regenerated plants and their progeny. EMBO J 3:1681–1689, 1984.
De Framond A, Barton K, Chilton M-D: MINI-Ti: A new vector strategy for plant genetic engineering. Bio/technology 1:262–269, 1983.
Dhaese P, De Greve H, Decraemer H, Schell J, Van Montagu M: Rapid mapping of transposon insertion and deletion mutations in the large Ti plasmids of Agrobacterium tumefaciens. Nucl Acids Res 7:1837–1849, 1979.
Ditta G, Stanfield S, Corbin D, Helinski D: Broad-host range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77:7347–7351, 1980.
Fraley R, Rogers S, Horsch R, Sanders P, Flick J, Adams S, Bittner M, Brand L, Fink C, Fry J, Galluppi G, Goldberg S, Hoffman N, Woo S: Expression of a bacterial gene in plant cells. Proc Natl Acad Sci USA 80:4803–4807, 1983.
Fraley R, Horsch R, Matzke A, Chilton M-D, Chilton W, Sanders P: In vitro transformation of petunia cells by an improved method of co-cultivation with A. tumefaciens strains. Plant Mol Biol 3:371–378, 1984.
Fraley R, Rogers S, Horsch R, Eichholtz D, Flick J, Fink C, Hoffman N, Sanders P: The SEV system: A new disarmed Ti plasmid vector system for plant transformation. Bio/technology 3:629–635, 1985.
Garfinkel D, Simpson R, Ream L, White F, Gordon M, Nester E: Genetic analysis of crown gall: Fine structure map of the T-DNA by site-directed mutagenesis. Cell 27:143–153, 1981.
Hepburn A, Clarke L, Blundy K, White J: Nopaline Ti plasmid, pTiT37, T-DNA insertions into a flax genome. J Mol Appl Genet 2:211–224, 1983.
Hoekema A, Hirsch P, Hooykaas P, Schilperoort R: A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti plasmid. Nature 303:179–180, 1983.
Hoekema A, van Haaren M, Fellinger A, Hooykaas P, Schilperoort R: Non-oncogenic plant vectors for use in the Agrobacterium binary system. Plant Mol Biol 5:85–89, 1985.
Horsch R, Fry J, Hoffman N, Eichholtz D, Rogers S, Fraley R: A simple and general method for transferring genes into plants. Science 227:1229–1231, 1985.
Jacoby G, Jacob A, Hedges R: Recombination between plasmids of incompatibility groups P1 and P2. J Bacteriol 127:1278–1285, 1976.
Klee H, Yanofsky M, Nester E: Vectors for transformation of higher plants. Bio/technology 3:637–642, 1985.
Leemans J, Shaw C, Deblaere R, De Greve H, Hernalsteens J, Maes M, Van Montagu M, Schell J: Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells. J Mol Appl Genet 1:149–164, 1981.
Matzke A, Chilton M-D: Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid: An approach to genetic engineering of higher plant cells. J Mol Appl Genet 1:39–49, 1981.
Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15:473–597, 1962.
Otten L, Schilperoort R: A rapid microscale method for the detection of lysopine and nopaline dehydrogenase activities. Biochem Biophys Acta 527:497–500, 1978.
Rogers S, Fraley R, Horsch R, Levine A, Flick J, Brand L, Fink C, Mozer T, O'Connell K, Sanders P: Evidence for ribosome scanning during translation initiation of mRNAs in transformed plant cells. Plant Mol Biol Rep 3:111–116, 1985.
Rothstein S, Jorgensen R, Postle K, Reznikoff W: The inverted repeats of Tn5 are functionally different. Cell 19:795–805, 1980.
Van den Elzen P, Lee K, Townsend J, Bedbrook J: Simple binary vectors for DNA transfer to plant cells. Plant Mol Biol 5:149–154, 1985.
Waldron C, Murphy E, Roberts J, Gustafson G, Armour S, Malcolm S: Resistance to hygromycin B. Plant Mol Biol 5:103–108, 1985.
Zambryski P, Joos H, Genetello C, Leemans J, Van Montagu M, Schell J: Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J 2:2143–2150, 1983.
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Matzke, A.J.M., Matzke, M.A. A set of novel Ti plasmid-derived vectors for the production of transgenic plants. Plant Mol Biol 7, 357–365 (1986). https://doi.org/10.1007/BF00032565
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DOI: https://doi.org/10.1007/BF00032565