Abstract
Plantlet regeneration in Cucumis metuliferus from several explant sources, including cotyledons, leaves, hypocotyls and petioles, was evaluated on Murashige and Skoog's medium containing various combinations of auxin (IAA, NAA, 2,4-d) and cytokinin (BA, kinetin, zeatin), Callus development was obtained within 4 to 5 weeks on all growth regulator combinations which were tested at concentrations ranging from 1.0 μM to 4.0 μM of each. The response was similar when the tissues were incubated under light or in continuous darkness. Differentiation of callus to form adventitious buds or shoot primordia occurred only with petiole explants on medium containing NAA/BA or 2,4-d/BA at 2.0/1.0 μM; none of these calluses, however, differentiated further to form shoots. When the differentiated calluses derived from petiole explants which had been initiated on 2,4-d/BA at 2.0/1.0 μM were transferred onto medium with 2.0 μM zeatin, formation of shoots occurred within 2 to 3 weeks. The frequency of shoot formation was 14.6%. Subculture of these shoots onto MS medium without growth regulators gave rise to plantlets of normal appearance. Regeneration in C. metuliferus requires callus initiation on an appropriate growth regulator regime followed by transfer to a medium containing the cytokinin, zeatin, and can be achieved within 10–12 weeks.
Similar content being viewed by others
Abbreviations
- BA:
-
6-benzylaminopurine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- NAA:
-
napthaleneacetic acid
References
Benzioni A, Mendlinger S & Ventura M (1991) Effect of sowing dates, temperatures on germination, flowering, and yield of Cucumis metuliferus. HortScience 26: 1051–1053.
Chee PP (1991) Plant regeneration from cotyledons of Cucumis melo ‘Topmark’. HortScience 26: 908–910.
Dabauza M, Roig LA & Moreno V (1991) Selective methods for the recovery of somatic hybrids of Cucumis melo × C. metuliferus and C. sativus × C. metuliferus. Cucurbit Genet. Coop. Rep. 14: 81–84.
Esquinas-Alcazar JT & Gilick PJ (1983) Genetic Resources of Cucurbitaceae (p. 101). International Board for Plant Genetic Resources Bulletin, FAO/UN, Rome, Italy.
Fassuliotis G (1967) Species of Cucumis resistant to the root-knot nematode Meloidogyne incognita acrita. Plant Dis. Rep. 51: 720–723.
Kim S-G, Chang J-R, Cha HC & Lee K-W (1988) Callus growth and plant regeneration in diverse cultivars of cucumber (Cucumis sativus L.). Plant Cell Tiss. Org. Cult. 12: 67–74.
McCarthy WH, Wehner TC & Daub ME (1989) Isolation and culture of Cucumis metuliferus protoplasts. Cucurbit Genet. Coop. Rep. 12: 26–29.
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
Orczyk W, Nadolska-Orczyk A & Malepzy S (1988) Plant regeneration of wild Cucumis species. Acta. Societ. Botanic Poloniae 57: 195–200.
Provvidenti R & Robinson RW (1974) Resistance to squash mosaic and watermelon mosaic virus 1 in Cucumis metuliferus. Plant Dis. Rep. 58: 735–738.
Punja ZK, Tang TA & Watkins LH (1988) Identification of resistance to root knot nematodes and virus diseases in Cucumis metuliferus and approaches to hybridization with Cucumis sativus by protoplast fusion. Phytopath. 78: 1578 (abstr.).
Punja ZK, Abbas N, Sarmento GG & Tang FA (1990) Regeneration of Cucumis sativus var. sativus and C. sativus var. hardwickii, C. melo, and C. metuliferus from explants through somatic embryogenesis and organogenesis. Plant Cell Tiss. Org. Cult. 21: 93–102.
Punja ZK & Raharjo SHT (1992) Regeneration of plants from protoplasts of Cucumis sativus L. (eucumber). In: Bajaj YPS (Ed) Plant Protoplasts and Genetic Engineering, Vol 3. Springer-Verlag Berlin.
Staub JE & Kupper RS (1985) Use of Cucumis sativus var. hardwickii germplasm in backcrosses with Cucumis sativus var. sativus. HortScience 20: 436–438.
Tang FA & Punja ZK (1989) Isolation and culture of protoplasts of Cucumis sativus and Cucumis metuliferus and methods for their fusion. Cucurbit Genet. Coop. Rep. 12: 29–34.
Wehner TC, Cade RM & Locy RD (1990) Cell, tissue and organ culture techniques for genetic improvement of cucurbits. In: Bates DM, Robinson RW & Jeffrey C (Eds) Biology and Utilization of Cucurbitaceae (pp 367–381). Cornell Univ Press.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Raharjo, S.H.T., Punja, Z.K. Plantlet regeneration from petiole explants of the african horned cucumber, Cucumis metuliferus . Plant Cell Tiss Organ Cult 32, 169–174 (1993). https://doi.org/10.1007/BF00029839
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00029839