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Localization of the VirA domain involved in acetosyringone-mediatedvir gene induction inAgrobacterium tumefaciens

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Abstract

The VirA protein ofAgrobacterium tumefaciens is thought to be a receptor for plant phenolic compounds such as acetosyringone. Although it is not known whether the interaction between VirA and the phenolics is direct or requires other phenolic-binding proteins, it is shown in this study that the first 280 amino acids of the VirA protein are not essential for the acetosyringone mediatedvir gene induction response. Considering the fact that the cytoplasmic region between the amino acids 283 and 304 is highly conserved between the different VirA proteins, and that deletion of this region abolishes VirA activity, we suggest that the acetosyringone receptor domain is located in this cytoplasmic domain of the VirA protein.

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Author notes

  1. Stefan C. H. J. Turk

    Present address: Department of Molecular Cell Biology, University of Utrecht, Padualaan 8, 3584 CH, Utrecht, Netherlands

Authors and Affiliations

  1. Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, Netherlands

    Stefan C. H. J. Turk, Richard P. van Lange, Tonny J. G. Regensburg-Tuïnk & Paul J. J. Hooykaas

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  1. Stefan C. H. J. Turk
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  2. Richard P. van Lange
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  3. Tonny J. G. Regensburg-Tuïnk
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  4. Paul J. J. Hooykaas
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Turk, S.C.H.J., van Lange, R.P., Regensburg-Tuïnk, T.J.G. et al. Localization of the VirA domain involved in acetosyringone-mediatedvir gene induction inAgrobacterium tumefaciens . Plant Mol Biol 25, 899–907 (1994). https://doi.org/10.1007/BF00028884

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