Skip to main content
SpringerLink
Log in

Somatic embryogenesis and plant development from anther culture of Vitis vinifera (L.)

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Anthers from Frumoasa alba (White beauty), Otilia, Valerien, Mission and Siegfried Rebe (FS4) cultivars were cultured at the uninucleate stage of the microspore on Murashige and Skoog (1962) and Nitsch and Nitsch (1969) media supplemented with 2,4-dichlorophenoxyacetic acid (4.9 μM) and benzyladenine (4.4 μM). The primary calli were subcultured on MS medium with 6.6 μM BA and 1.1 μM indolylacetic acid, in order to induce their growth and plant regeneration. After seven months, vegetative buds were obtained with Frumoasa alba (2.7%), Otilia (0.3%), Valerien (4.5%), embryogenic callus was obtained with Mission and plant regeneration with Siegfried Rebe. Long term embryogenesis was maintained in Mission cv. for four years, by selection and regular transfer of the embryogenic areas of anther-derived calli. The embryogenic calli have the ability to generate abnormal somatic embryos with one, two or three cotyledons and cup or trumpet-shaped with fused cotyledons. In parallel with the embryogenic process, organogenesis with buds, leaf and shoot differentiation was regularly observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Altamura MM, Cersosimo A, Majoli C and Crespan M (1992) Histological study of embryogenesis and organogenesis from anthers of Vitis rupestris du Lot cultured in vitro. Protoplasma 171: 134–141

    Google Scholar 

  2. Barlass M and Skene KGM (1978) In vitro propagation of grapevine (Vitis vinifera L.) from fragmented shoot apices. Vitis 17: 335–340

    Google Scholar 

  3. Barwale UB, Kerns HR and Widholm JM (1986) Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 167: 473–481

    Google Scholar 

  4. Brown MV, Moore NJ and Huang FH (1994) Effects of NAA, 2,4-D and BAP on callus formation in table grapes as influenced by species composition. HortScience 29(5): 548

    Google Scholar 

  5. Emershad RL and Ramming DW (1994) Somatic embryogenesis and plant development from immature zygotic embryos of seedless grapes (Vitis vinifera L.). Plant Cell Reports 14: 6–12

    Google Scholar 

  6. Faure O (1990) Embryons somatiques de Vitis rupestris et embryons zygotiques de Vitis sp.: Morphologie, histologie, histochimie et development. Can J Bot 68: 2305–2315

    Google Scholar 

  7. Goebel-Tourand J, Mauro MC, Sossountzov L, Miginiac E and Deloire A (1993) Arrest of somatic embryos development in grapevine: Histological characterisation and the effect of ABA, BAP and zeatin in stimulating plantlet development. Plant Cell, Tissue Organ Culture 33: 91–103

    Google Scholar 

  8. Gray DJ and Mortensen JA (1987) Initiation and maintenance of long term somatic embryogenesis from anthers and ovaries of Vitis longii “Microsperma” Plant Cell Tissue Organ Culture 9: 73–80

    Google Scholar 

  9. Hirabayashi T, Kozaki I and Akihama T (1976) In vitro differentiation of shoots from anther callus in Vitis. Hort Sci 11: 511–512

    Google Scholar 

  10. Newton DJ and Gonssard PG (1990) The ontogeny of somatic embryos from in vitro cultured grapevine anthers. S Afr J Enol Vitic 11(2): 70–75

    Google Scholar 

  11. Popescu CF, Baditescu D and Visoiu E (1991) Preliminary results concerning somatic embryogenesis in vitro from anther derived callus. Ann Rep ICVV 13: 11–21

    Google Scholar 

  12. Scorza R, Cordts JM, Ramming DW and Emershad RL (1994) Transformation of grape (Vitis vinifera L.) somatic embryos and regeneration of transgenic plants. J Cel Biochem Suppl 18A: 102

    Google Scholar 

  13. Srinivasan C and Mullins MG (1980) High-frequency somatic embryo production from unfertilized ovules of grapes. Sci Hort 13: 245–252

    Google Scholar 

  14. Stamp JA and Meredith CP (1988) Proliferative somatic embryogenesis from zygotic embryos of grapevine. J Amer Soc Hort Sci 113: 941–945

    Google Scholar 

  15. Tang FA and Mullins MG (1990) Adventitious bud formation in leaf explants of some grapevine rootstock and scion cultivars. Vitis 29: 151–158

    Google Scholar 

  16. Vasil V and Vasil IK (1982) Characterisation of an embryogenic cell suspension culture derived from cultured inflorescences of Pennisetum americanum (Pearl Millet), L., gramineae). Am J Bot 69: 1441–1449

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Station for Viticulture, 0343, Stefanesti-Arges, Romania

    Carmen Florentina Popescu

Authors
  1. Carmen Florentina Popescu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Popescu, C.F. Somatic embryogenesis and plant development from anther culture of Vitis vinifera (L.). Plant Growth Regul 20, 75–78 (1996). https://doi.org/10.1007/BF00024062

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00024062

Key words

Search

Navigation