Abstract
The paper compares different approaches for the genetic transformation of cauliflower (Agrobacterium-mediated, PEG-mediated and/or electroporation). Transient expression of the neomycin phosphotransferase II (NPTII) gene could be detected after direct gene transfer. Stable transformation was achieved using both Agrobacterium-mediated and direct gene transfer. Expression as well as incorporation of the NPTII sequence could be demonstrated.
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Eimert, K., Siegemund, F. Transformation of cauliflower (Brassica oleracea L. var. botrytis) — an experimental survey. Plant Mol Biol 19, 485–490 (1992). https://doi.org/10.1007/BF00023396
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DOI: https://doi.org/10.1007/BF00023396