Summary
Random amplified polymorphic DNA (RAPD) markers have been successfully employed to analyse the genetic diversity among cultivated and subspontaneous accessions of Coffea arabica. The narrow genetic base of commercial cultivars was confirmed. On the other hand, a relatively large genetic diversity was observed within the germplasm collection demonstrating the importance of collecting missions. Results suggested an East-West differentiation in Ethiopia, the primary centre of diversification of C. arabica. The large heterosis effect reported in intergroup hybrids could be related to such genetic differentiation. RAPD method appeared to be effective in resolving genetic variations and in grouping germplasm in C. arabica.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berthaud J. & A.Charrier, 1988. Genetics resources of Coffea. In: R.J.Clarke & R.Macrae (Eds) Coffee vol 4: Agronomy. Elsevier Applied Science, London, pp. 1–42.
Berthaud J., J.L.Guillaumet, D.LePierres & M.Lourd, 1980. Les caféiers sauvages du Kenya: prospection et mise en culture. Café-Cacao-Thé 24: 101–112.
Berthou, F. & P. Trouslot, 1977. L'analyse du polymorphisme enzymatique dans le genre Coffea: adaptation d'une méthode d'électrophorèse en série. 8th Conference of ASIC, Abidjan (Côte d'Ivore): 373–383.
Bayetta-Bellachew, Mesfin-Ameha & Dejene-Mekonnen, 1993. Heterosis and combining ability in coffee (Coffea arabica L.). 15th Conference of ASIC, Montpellier (France): 234–242.
Bouharmont P. & C. Montagnon, 1995. Diversité phenotypique de Coffea arabica observée en collection au Cameroun. 16th Conference of ASIC, Kyoto (Japan), in press.
Carvalho A., 1988. Principles and practices of coffee plant breeding for productivity and quality factors: Coffea arabica. In: R.J.Clarke & R.Macrae (Eds) Coffee vol 4: Agronomy. Elsevier Applied Science, London, pp. 129–165.
Carvalho A., H.P. MedinaFilho, L.C.Fazuoli, O. GuerreiroFilho & M.M.A.Lima, 1991. Aspectos genéticos do cafeeiro. Rev. Brasil. Genet. 14: 135–183.
Charrier, A., 1978. Synthèse de neuf années d'observation et d'expérimentation sur les Coffea arabica collectés en Ethiopie par une mission ORSTOM. In: A. Charrier (Ed.) Etude de la structure et de la variabilité génétique des caféiers, IFCC (Paris), Bulletin no 14, pp. 4–10.
Darlu P. & P.Tassy, 1993. Reconstruction phylogénétique: concepts et méthodes. Collection biologie théorique, Masson, Paris (France), 245 p.
F.A.O., 1968. FAO coffee mission to Ethiopia 1964–1965. Report, FAO ed., Rome (Italy), 200 p.
Guillaumet, J.L. & F. Hallé, 1978. Echantillonnage du materiel Coffea arabica récolté en Ethiopie. In: A. Charrier (Ed.) Etude de la structure et de la variabilité génétique des caféiers, IFCC (Paris), Bulletin no. 14, pp. 13–18.
Krug C.A., J.E.T.Mendes & A.Carvalho, 1939. Taxonomia de Coffea arabica L. Descriçao das variedase formas encontradas no estado de Sao Paulo. Boletim Tecnico 62, Instituto Agronomico do Estado (Campinas, Brazil) 57 p
Kruskal J.B., 1964a. Nonmetric multidimensional scaling: a numerical method. Psychometrika 29: 1–27.
Kruskal J.B., 1964b. Multidimensional scaling by optimizing goodness of fit to a nonmetric hypothesis. Psychometrika 29: 115–129.
Lashermes P., J.Cros, P.Marmey & A.Charrier, 1993. Use of random amplified DNA markers to analyze genetic variability and relationships of Coffea species. Genetic Resources and Crop Evolution 40: 91–99.
Lashermes, P., M.C. Combes, J. Cros, P. Trouslot, F. Anthony & A. Charrier, 1995. Origin and genetic diversity of Coffea arabica L. based on DNA molecular markers. 16th Conference of ASIC, Kyoto (Japan), in press.
Meyer, G.G., 1969. The origin of arabica coffee. Proc. XI intl. bot. Congr., Seattle (USA), 146 p.
Orozco-Castillo C., K.J.Chalmers, R.Waugh & W.Powell, 1994. Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor. Appl. Genet. 87: 934–940.
Shepard R.N., 1973. Introduction to vol. 1. In: R.N.Shepard, A.K.Romney & S.B.Nerlove (Eds) Multidimensional scaling, vol. 1, Seminar press, New York (USA).
Smith F.R., 1985. A history of coffee. In: M.N.Clifford & K.C.Wilson (Eds) Coffee, Botany, Biochemistry and production of beans and beverage. Croom Helm, London & Sydney, pp. 1–12.
Sylvain P.G., 1958. Ethiopian Coffee — Its significance to world coffee problems. Botany 12: 111–139.
Van derVossen H.A.M., 1985. Coffee selection and breeding. In: M.N.Clifford & K.C.Wilson (Eds) Coffee, Botany, Biochemistry and production of beans and beverage. Croom Helm, London & Sydney, pp. 48–97.
Walyaro, D.J.A., 1983. Considerations in breeding for improved yield and quality in arabica coffee (Coffea arabica L.). Doctoral thesis, Wageningen, The Netherlands, 122 p.
Welsh J. & M.McClelland, 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18: 7213–7218.
Williams J.G.K., A.R.Kubelik, K.J.Livak, J.A.Rafalski & S.V.Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531–6535.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lashermes, P., Trouslot, P., Anthony, F. et al. Genetic diversity for RAPD markers between cultivated and wild accessions of Coffea arabica . Euphytica 87, 59–64 (1996). https://doi.org/10.1007/BF00022965
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00022965