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The region distal to the rRNA operon from chloroplasts of maize contains genes coding for tRNAArg(ACG) and tRNAAsn(GUU)

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Summary

The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNAArg(ACG) and tRNAAsn(GUU). The tRNAArg(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation as the rRNA operon. By S1 and primer extension mapping, the existence of transcripts from the entire 5S rDNA/tDNAArg(ACG) intergenic region can be demonstrated. It is, therefore, concluded that tRNAArg(ACG) represents a trailer tRNA which is cotranscribed with 5S rRNA as part of the primary rRNA transcript. The tDNAAsn(GUU), which is separated from tDNAArg(ACG) by an intergenic region of 253 bp, has the opposite orientation with respect to the rRNA operon; it, therefore, represents a separate transcriptional unit whose promoter remains to be located. It is proposed that the two tRNA genes possess a common terminator region, which functions in both directions of transcription.

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References

  1. Bedbrook JR, Kolodner R, Bogorad L:Zea mays chloroplast ribosomal RNA genes are part of a 22000 base pair inverted repeat. Cell 11: 739–749, 1977.

    Google Scholar 

  2. Briat J-F, Dron M, Mache R: Is transcription of higher plant chloroplast ribosomal operons regulated by premature termination? FEBS Letters 163: 1–5, 1983.

    Google Scholar 

  3. Coleman J, Green PJ, Inouye M: The use of RNAs complementary to specific mRNAs to regulate the expression of individual bacterial genes. Cell 37: 429–436, 1984.

    Google Scholar 

  4. Crouse EJ, Schmitt JM, Bohnert HJ: Chloroplast and cyanobacterial genomes, genes and RNAs: a compilation. Plant Molec Biol Reporter 3: 43–89, 1985.

    Google Scholar 

  5. Fournier MU, Ozeki H: Structure and organization of the transfer ribonucleic acid genes ofEscherichia coli K-12. Microbiol Rev 49: 379–397, 1985.

    Google Scholar 

  6. Greenberg BM, Narita JO, De Luca-Flaherty C, Gruissem W, Rushlow KA, Hallick RB: Evidence for two RNA polymerase activities inEuglena gracilis chloroplasts. J Biol Chem 259: 14880–14887, 1984.

    Google Scholar 

  7. Gruissem W, Greenberg BM, Zurawski G, Prescott DM, Hallick RB: Biosynthesis of chloroplast transfer RNA in a spinach chloroplast transcription system. Cell 35: 815–828, 1983.

    Google Scholar 

  8. Gruissem W, Zurawski G: Identification and mutational analysis of the promoter for a spinach chloroplast transfer RNA gene. EMBO J 4: 1637–1644, 1985.

    Google Scholar 

  9. Hellmund D, Metzlaff M, Serfling E: A transfer RNAArg gene ofPelargonium chloroplasts, but not a 5S RNA gene, is efficiently transcribed after injection intoXenopus oocyte nuclei. Nucleic Acids Res 12: 8253–8268, 1984.

    Google Scholar 

  10. Kato A, Shimada H, Kusuda M, Sugiura M: The nucleotide sequences of two tRNAAsn genes from tobacco chloroplasts. Nucleic Acids Res 9: 5601–5607, 1981.

    Google Scholar 

  11. Kato A, Takaiwa F, Shinozaki K, Sugiura M: Location and nucleotide sequence of the genes for tobacco chloroplast tRNAArg(ACG) and tRNALeu(UAG). Curr Genet 9: 405–409, 1985.

    Google Scholar 

  12. Keus RJ, Stam NJ, Zwiers T, Heij HT, Groot GSP: The nucleotide sequences of the genes coding for tRNA Arg(UCU), tRNA Arg(ACG), and tRNA Asn(GUU) onSpirodela oligorhiza chloroplast DNA. Nucleic Acids Res 12: 5639–5646, 1984.

    Google Scholar 

  13. Koch W, Edwards K, Kössel H: Sequencing of the 16S–23S spacer in a ribosomal RNA operon ofZea mays chloroplast DNA reveals two split tRNA genes. Cell 25: 203–213, 1981.

    Google Scholar 

  14. Kössel H, Natt E, Strittmatter G, Fritzsche E, Gozdzicka-Jozefiak A, Przybyl D: Structure and expression of rRNA operons from plastids of higher plants. In: van Vloten-Doting L, Groot GSP, Hall TC (eds) Molecular Form and Function of the Plant Genome. Plenum Publishing Corporation, 1985, pp 183–198.

  15. Lamond AJ, Travers AA: Stringent control of bacterial transcription. Cell 41: 6–8, 1985.

    Google Scholar 

  16. Maxam AM, Gilbert W: Sequencing end-labelled DNA with base-specific chemical cleavages. Methods of Enzymology 65: 499–560, 1980.

    Google Scholar 

  17. Mubumbila M, Bowman CM, Droog F, Dyer T, Kuntz M, Weil HJ: Chloroplast transfer RNAs and tRNA genes of wheat. Plant Mol Biol 4: 315–320, 1985.

    Google Scholar 

  18. Przybyl D, Fritzsche E, Edwards K, Kössel H, Falk H, Thompson JA, Link G: The ribosomal RNA genes from chloroplasts of mustard (Sinapis alba L.): mapping and sequencing of the leader region. Plant Mol Biol 3: 147–158, 1984.

    Google Scholar 

  19. Rosenberg M, Court D: Regulatory sequences involved in the promotion and termination of RNA transcription. Ann Rev Genet 13: 319–353, 1979.

    Google Scholar 

  20. Schwarz Z, Kössel H, Schwarz E, Bogorad L: A gene coding for tRNAVal is located near 5′ terminus of 16S rRNA gene inZea mays chloroplast gene. Proc Natl Acad Sci USA 78: 4748–4752, 1981.

    Google Scholar 

  21. Sekiya T, Contreras R, Takeya T, Khorana HG: Total synthesis of a tyrosine suppressor transfer RNA gene. XVII. Transcription,in vitro, of the synthetic gene and processing of the primary transcript to transfer RNA. J Biol Chem 254: 5802–5816, 1979.

    Google Scholar 

  22. Selden RF, Steinmetz A, McIntosh L, Bogorad L, Burkard G, Mubumbila M, Kuntz M, Crouse EJ, Weil HJ: Transfer RNA genes ofZea mays chloroplast DNA. Plant Mol Biol 2: 141–153, 1983.

    Google Scholar 

  23. Shapiro DR, Tewari KK: Nucleotide sequences of transfer RNA genes in thePisum sativum chloroplast DNA. Plant Mol Biol 6: 1–12, 1986.

    Google Scholar 

  24. Strittmatter G, Gozdzicka-Jozefiak A, Kössel H: Identification of an rRNA operon promoter fromZea mays chloroplasts which excludes the proximal tRNAVal(GAC) from the primary transcript. EMBO J 4: 599–604, 1985.

    Google Scholar 

  25. Strittmatter G, Kössel H: Cotranscription and processing of 23S, 4.5S and 5S rRNA in chloroplasts fromZea mays. Nucleic Acids Res 12: 7633–7647, 1984.

    Google Scholar 

  26. Takaiwa F, Sugiura M: Nucleotide sequence of the 4.5S and 5S ribosomal RNA genes from tobacco chloroplasts. Molec Gen Genet 180: 1–4, 1980.

    Google Scholar 

  27. Travers A: Regulation by anti-sense RNA. Nature 311: 410, 1984.

    Google Scholar 

  28. Whitfeld PR, Bottomley W: Organization and structure of chloroplast genes. Ann Rev Plant Physiol 34: 279–310, 1983.

    Google Scholar 

  29. Whitfeld PR, Herrmann RG, Bottomley W: Mapping of the ribosomal RNA genes on spinach chloroplast DNA. Nucleic Acids Res 5: 1741–1751, 1978.

    Google Scholar 

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Authors and Affiliations

  1. Institut für Biologie III der Universität Freiburg, Schänzlestr. 1, D-7800, Freiburg, Federal Republic of Germany

    D. Dormann-Przybyl, G. Strittmatter & H. Kössel

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  1. D. Dormann-Przybyl
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  2. G. Strittmatter
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  3. H. Kössel
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This paper is dedicated to Professor Morio Ikehara on the occasion of his retirement from Osaka University in March 1986.

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Dormann-Przybyl, D., Strittmatter, G. & Kössel, H. The region distal to the rRNA operon from chloroplasts of maize contains genes coding for tRNAArg(ACG) and tRNAAsn(GUU). Plant Mol Biol 7, 419–431 (1986). https://doi.org/10.1007/BF00020326

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  • DOI: https://doi.org/10.1007/BF00020326

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