Skip to main content
SpringerLink
Log in

A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants

  • Research Article
  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

A full-length tomato cDNA clone, TSW12, which is developmentally and environmentally regulated, has been isolated and characterized. TSW12 mRNA is accumulated during tomato seed germination and its level increases after NaCl treatment or heat shock. In mature plants, TSW12 mRNA is only detected upon treatment with NaCl, mannitol or ABA and its expression mainly occurs in stems. The nucleotide sequence of TSW12 includes an open reading frame coding for a basic protein of 114 amino acids; the first 23 amino acids exhibit the sequence characteristic of a signal peptide. The high similarity between the TSW12-deduced amino acid sequence and reported lipid transfer proteins suggests that TSW12 encodes a lipid transfer protein.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bandziulis RJ, Swanson MS, Dreyfuss G: RNA-binding proteins as developmental regulators. Genes Devel 3: 431–437 (1989).

    PubMed  Google Scholar 

  2. Bernhard WR, Somerville CR: Coidentity of putative amylase inhibitors from barley and finger millet with phospholipid transfer proteins inferred from amino acid sequence homology. Arch Biochem Biophys 269: 695–697 (1989).

    PubMed  Google Scholar 

  3. Bernhard WR, Thoma S, Botella J, Somerville CR: Isolation of cDNA clone for spinach lipid transfer protein and evidence that the protein is synthesized by the secretory pathway. Plant Physiol 95: 164–170 (1991).

    Google Scholar 

  4. Binzel ML, Hasegawa PM, Rhodes D, Handa S, Handa AK, Bressan RA: Solute accumulation of tobacco cells adapted to NaCl. Plant Physiol 84: 1408–1415 (1987).

    Google Scholar 

  5. Bouillon P, Drischel C, Vergnolle C, Duranton H, Kader JC: The primary structure of spinach-leaf phospholipid-transfer protein. Eur J Biochem 166: 387–391 (1987).

    PubMed  Google Scholar 

  6. Breu V, Guerbette F, Kader J-C, Kannangara C, Svensson B, vonWettstein-Knowles B: A 10 kd barley basic protein transfer phosphatidylcholine from liposomes to mitochondrias. Carlsberg Res Commun 54: 81–84 (1989).

    Google Scholar 

  7. Campos FAP, Richardson M: The complete amino acid sequence of the α-amylase inhibitor I-2 from seeds of ragi (Indian finger millet,Eleusine coracana Gaern.). FEBS Lett 167: 221–225 (1984).

    Article  Google Scholar 

  8. Czarnecka E, Edelman I, Schöffl F, Key JL: Comparative analysis of physical stress responses in soybean seedlings using cloned heat shock cDNAs. Plant Mol Biol 3: 45–58 (1984).

    Google Scholar 

  9. Delcasso-Tremousaygue D, Grellet F, Panabieres F, Ananiev ED, Delseny M: Structural and transcriptional characterization of the external spacer of a ribosomal RNA nuclear gene from a higher plant. Eur J Biochem 172: 277–291 (1988).

    Google Scholar 

  10. Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).

    PubMed  Google Scholar 

  11. DureIII L, Crouch M, Harada J, Ho THD, Mundy J, Quatrano R, Thomas T, Sung ZR: Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol Biol 12: 475–486 (1989).

    Google Scholar 

  12. Garnier J, Osguthorpe DJ, Robson B: Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol 120: 97–120 (1978).

    PubMed  Google Scholar 

  13. Godoy JA, Pardo JM and Pintor-Toro JA: A tomato cDNA inducible by salt stress and abscisic acid: nucleotide sequence and expression pattern. Plant Mol Biol 15: 695–705 (1990).

    Article  PubMed  Google Scholar 

  14. Gomez J, Sanchez-Martinez D, Stiefel V, Rigau J, Puigdomenech P, Pagés M: A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature 334: 262–264 (1988).

    PubMed  Google Scholar 

  15. Harrington HM, Alm DM: Interaction of heat and salt shock in cultured tobacco cells. Plant Physiol 88: 618–625 (1988).

    Google Scholar 

  16. Heikkila JJ, Papp JET, Schultz GA, Bewley JD: Induction of heat shock protein messenger RNA in maize mesocotyls by water stress, abscisic acid, and wounding. Plant Physiol 76: 270–274 (1984).

    Google Scholar 

  17. Hirayama O, Mihara M: Characterization of membrane lipids of higher plants differing in salt-tolerance. Agric Biol Chem 51: 3215–3221 (1987).

    Google Scholar 

  18. Kader JC: Intracellular transfer of phospholipids, galactolipids, and fatty acids in plant cells. In: Hilderson HJ (ed) Subcellular Biochemistry, pp. 69–111. Plenum, New York (1990).

    Google Scholar 

  19. King GJ, Turner VA, Hussey CE, Wurtele ES, Lee SM: Isolation and characterization of a tomato cDNA clone which codes for a salt-induced protein. Plant Mol Biol 10: 401–412 (1988).

    Google Scholar 

  20. Kuiper PJC: Functioning of plant cell membranes under saline conditions: membrane lipid composition and ATPases. In: Staples RC, Toenniessen GH (eds) Salinity Tolerance in Plants: Strategies for Crop Improvement, pp. 77–91. John Wiley Canada (1984).

    Google Scholar 

  21. Leah R, Mundy J: The bifunctional a-amylase/subtilisin inhibitor of barley: nucleotide sequence and patterns of seed-specific expression. Plant Mol Biol 12: 673–682 (1989).

    Google Scholar 

  22. Leopold AC, Wiling RP: Evidence for toxicity effects of salt on membranes. In: Staples RC, Toenniessen GH (eds) Salinity Tolerance in Plants: Strategies for Crop Improvement, pp. 77–91. John Wiley Canada (1984).

    Google Scholar 

  23. Murray MG, Thompson WF: Rapid isolation of high molecular weight plant DNA. Nucl Acids Res 8: 4321–4325 (1980).

    PubMed  Google Scholar 

  24. Paternak D: Salt tolerance and crop production-a comprehensive approach. Annu Rev Phytopath 25: 271–291 (1987).

    Google Scholar 

  25. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).

    Google Scholar 

  26. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    PubMed  Google Scholar 

  27. Schwartz-Sommer Z, Gierl A, Cuypers H, Peterson PA, Saedler H: Plant transposable elements generate the DNA sequence diversity needed in evolution. EMBO J 4: 591–597 (1985).

    Google Scholar 

  28. Singh NK, Bracker CA, Hasegawa PM, Handa AK, Buckel S, Hermodson MA, Regnier F, Bressan R: Characterization of osmotin: a thaumatin-like protein associated with osmotic adaptation in plant cells. Plant Physiol 85: 529–536 (1987).

    Google Scholar 

  29. Singh NK, Nelson DE, Kuhn D, Hasegawa PM, Bressan RA: Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential. Plant Physiol 90: 1096–1101 (1989).

    Google Scholar 

  30. Skriver K, Mundy J: Gene expression in response to abscisic acid and osmotic stress. The Plant Cell 2: 503–512 (1990).

    Article  PubMed  Google Scholar 

  31. Steward CR, Voetberg G: Relationship between stress-induced ABA and proline accumulations and ABA-induced proline accumulation in excised barley leaves. Plant Physiol 79: 24–27 (1985).

    Google Scholar 

  32. Takishima K, Watanabe S, Yamada M, Mamiya G: The amino-acid sequence of the nonspecific lipid transfer protein from germinated castor bean endosperms. Biochim Biophys Acta 870: 248–255 (1986).

    Google Scholar 

  33. Tchang F, This P, Stiefel V, Arondel V, Morch MD, Pagés M, Puigdomenech P, Grellet F, Delseny M, Bouillon P, Huet JC, Guerbette F, Beauvais-Cante F, Duranton H, Pernollet JC, Kader JC: Phospholipid transfer protein: full-length cDNA and amino acid sequence in maize. J Biol Chem 263: 16849–16855 (1988).

    PubMed  Google Scholar 

  34. vonHeijne G: Patterns of amino acids near signalsequence cleavage sites. Eur J Biochem 133: 17–21 (1983).

    PubMed  Google Scholar 

  35. Wadsworth GJ, Redinbaugh MG, Scandalios JG: A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis. Anal Biochem 172: 279–283 (1988).

    PubMed  Google Scholar 

  36. Watad A-EA, Pesci PA, Reinhold L, Lerner Hr: Proton fluxes as a response to external salinity in wild type and NaCl-adaptedNicotiana cell lines. Plant Physiol 81: 454–459 (1986).

    Google Scholar 

  37. Wirtz KWA, GadellaJr TWJ: Properties and mode of action of specific and non-specific phospholipid transfer proteins. Experientia 46: 592–598 (1990).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Recursos Naturales y Agrobiología, C.S.I.C, Apartado 1052, Sevilla, Spain

    Sonia Torres-Schumann, José A. Godoy & José A. Pintor-Toro

Authors
  1. Sonia Torres-Schumann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. José A. Godoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. José A. Pintor-Toro
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Torres-Schumann, S., Godoy, J.A. & Pintor-Toro, J.A. A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants. Plant Mol Biol 18, 749–757 (1992). https://doi.org/10.1007/BF00020016

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00020016

Key words

Search

Navigation