Abstract
In this article it is shown that the T-DNA of Agrobacterium tumefaciens contains besides the well-known cyt and aux genes another gene with an oncogenic effect in plants. The gene in question is called 6b and causes the formation of small tumors in plant species such as Nicotiana glauca and Kalanchoe tubiflora.
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Barker RF, Idler KB, Thompson DV, Kemp JD: Nucleotide sequence of the T-DNA region from Agrobacterium tumefaciens octopine Ti plasmid pTil5955. Plant Mol Biol 2: 335–350 (1983).
Bevan M: Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12: 8711–8721 (1984).
DeVos G, DeBeuckeleer M, VanMontagu M, Schell J: Restriction endonuclease mapping of the octopine tumor-inducing plasmid pTiAch5 of Agrobacterium tumefaciens. Plasmid 6: 249–253 (1981).
Garfinkel DJ, Simpson RB, Ream LW, White EF, Gordon MP, Nester EW: Genetic analysis of crown gall; fine structure map of the T-DNA by site-directed mutagenesis. Cell 27: 143–153 (1981).
Hille J, Wullems G, Schilperoort R: Non-oncogenic T-region mutants of Agrobacterium tumefaciens do transfer T-DNA into plant cells. Plant Mol Biol 2: 155–163 (1983).
Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA: A binary plant vector strategy based on separation of vir and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303: 179–180 (1983).
Hoekema A, VanHaaren MJJ, Fellinger AJ, Hooykaas PJJ, Schilperoort RA: Non-oncogenic plant vectors for use in the Agrobacterium binary system. Plant Mol Biol 5: 85–89 (1985).
Hooykaas PJJ, Hofker M, denDulk-Ras H, Schilperoort RA: A comparison of virulence determinants in an octopine Ti plasmid, a nopaline Ti plasmid, and an Ri plasmid by complementation analysis of Agrobacterium tumefaciens mutants. Plasmid 11: 195–205 (1984).
Joos H, Caplan A, Sormann M, VanMontagu M, Schell J: Genetic analysis of T-DNA transcripts in nopaline crown galls. Cell 32: 1057–1067 (1983).
Maniatis T, Fritsch EF, Sambrook J: Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Melchers LS, Hooykaas PJJ: Virulence of Agrobacterium. In: Miflin BJ (ed) Oxford Surveys of Plant Molecular and Cell Biology, Vol 4 pp 167–220. Oxford University Press, London (1987).
Messens E, Lenaerts A, VanMontagu M, Hedges RW: Genetic basis for opine secretion from crown gall tumour cells. Mol Gen Genet 199: 344–348 (1985).
Peerbolte R, Leenhouts K, Hooykaas-Van Slogteren GMS, Hoge JHC, Wullems GJ, Schilperoort RA: Clones from a shooty tobacco crown gall tumor I: deletions, rearrangements and amplifications resulting in irregular T-DNA structures and organizations. Plant Mol Biol 7: 265–284 (1986).
Ream LW, Gordon MP, Nester EW: Multiple mutations in the T region of the Agrobacterium tumefaciens tumor-inducing plasmid. Proc Natl Acad Sci USA 80: 1660–1664 (1983).
Vanderleyden J, Desair J, DeMeirsman C, Michiels K, VanGool A, Jen G, Chilton M-D: Nucleotide sequence of the T-DNA region encoding transcripts 6a and 6b of the pTiT37 nopaline Ti plasmid. Plant Mol Biol 7: 33–41 (1986).
Yanofsky M, Montoya A, Knauf V, Lowe B, Gordon M, Nester E: Limited-host-range plasmid of Agrobacterium tumefaciens: molecular and genetic analysis of transferred DNA. J Bact 163: 341–348 (1985).
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Hooykaas, P.J.J., den Dulk-Ras, H. & Schilperoort, R.A. The Agrobacterium tumefaciens T-DNA gene 6b is an onc gene. Plant Mol Biol 11, 791–794 (1988). https://doi.org/10.1007/BF00019519
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DOI: https://doi.org/10.1007/BF00019519