Abstract
The Fenna-Matthews-Olson (FMO)-protein and the FMO-reaction center (RC) core complex from the green sulfur bacterium Chlorobium tepidum were examined at 6 K by absorption and fluorescence spectroscopy. The absorption spectrum of the RC core complex was obtained by a subtraction method and found to have fiye peaks in the QY region, at 797, 808, 818, 834 and 837 nm. The efficiency of energy transfer from carotenoid to bacteriochlorophyll a in the RC core complex was 23% at 6 K, and from the FMO-protein to the core it was 35%. Energy transfer from the FMO-protein to the core complex was also measured in isolated membranes of Prosthecochloris aestuarii from the action spectra of charge separation. Again, a low efficiency of energy transfer was obtained, both at 6 K and at room temperature.
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Abbreviations
- BChl-:
-
bacteriochlorophyll
- P840-:
-
primary electron donor
- RC-:
-
reaction center
- FMO-protein-:
-
Fenna-Matthews-Olson-protein
References
Blankenship RE, Olson JM and Miller M (1995) Antenna complexes from green photosynthetic bacteria. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria, pp 399–435. Kluwer Academic Publishers, Dordrecht
Borisov AY (1990) Energy migration in purple bacteria. The criterion for discrimination between migration- and trapping-limited photosynthetic units. Photosynth Res 23: 283–289
Feiler U and Hauska G (1995) Green sulfur bacterial reaction centers. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria, pp 665–685. Kluwer Academic Publishers, Dordrecht
Feiler U, Nitschke W and Michel H (1992) Characterization of an improved reaction center preparation from the photosynthetic green sulfur bacterium Chlorobium containing the FeS centers FA and FB and a bound cytochrome subunit. Biochemistry 31: 2608–2614
Feiler U, Albouy D, Pourcet C, Mattioli T, Lutz M and Robert B (1994) Structure and binding site of the primary electron acceptor in the reaction center of Chlorobium. Biochemistry 33: 7594–7599
Francke C, Otte SCM, van derHeiden JC and Amesz J (1994) Spurious circular dichroism signals with intact cells of heliobacteria. Biochim Biophys Acta 1186: 75–80
Hager-Braun C, Xie DL, Jarosch U, Herold E, Büttner M, Zimmermann R, Deutzmann R, Hauska G and Nelson N (1995) Stable photobleaching of P840 in Chlorobium reaction center preparations: Presence of the 42-kDa bacteriochlorophyll alpha protein and a 17-kDa polypeptide. Biochemistry 34: 9617–9624
Holt SC, Conti SF and Fuller RC (1966) Effects of the light intensity on the formation of the photochemical apparatus in the green bacterium Chloropseudomonas ethylicum. J Bacteriol 91: 311–323
Hurt EC and Hauska G (1984) Purification of membrane-bound cytochromes and a photoactive P840 protein complex of the green sulfur bacterium Chlorobium liicola f. thiosulfatophilum. FEBS Lett 168: 149–154
Karapetyan NV, Swarthoff T, Rijgersberg CP and Amesz J (1980) Fluorescence emission spectra of cells and subcellular preparations of a green photosynthetic bacterium. Effects of dithionite on the intensity of the emission bands. Biochim Biophys Acta 593: 254–260
Kleinherenbrink FAM, Deinum G, Otte SCM, Hoff AJ and Amesz J (1992) Energy transfer from long-wavelength absorbing antenna bacteriochlorophylls to the reaction center. Biochim Biophys Acta 1099: 175–181
Kramer H, Aartsma TJ and Amesz J (1996) Excited states and charge separation in membranes of the green sulfur bacterium Prosthecochloris aestuarii. Photochem Photobiol 64: 26–31
Kramer HJM, Kingma H, Swarthoff T and Amesz J (1982) Prompt and delayed fluorescence in pigment-protein complexes of a green photosynthetic bacterium. Biochim Biophys Acta 681: 359–364
Miller M, Cox RP and Olson JM (1994) Low-temperature spectroscopy of isolated FMO-protein and a membrane-free reaction center complex from the green sulfur bacterium Chlorobium tepidum. Photosynth Res 41: 97–103
Nuijs AM, Vasmel H, Joppe HLP, Duysens LNM and Amesz J (1985) Excited states and primary charge separation in the pigment system of the green photosynthetic bacterium Prosthecochloris aestuarii as studied by picosecond absorbance difference spectroscopy. Biochim Biophys Acta 807: 24–34
Oh-oka H, Kakutani S, Kamei S, Matsubara H, Iwaki M and Itoh S (1995a) Highly purified photosynthetic reaction center (PscA/Cytochrome c 551)2 complex of the green sulfur bacterium Chlorobium limieola. Biochemistry 34: 13091–13097
Oh-oka H, Kamei S, Matsubara H, Iwaki M and Itoh S (1995b) Two molecules of cytochrome c function as the electron donors to P840 in the reaction center complex isolated from a green bacterium, Chlorobium tepidum. FEBS Lett 365: 30–34
Okkels JS, Kjær B, Hansson Ö, Svendsen I, Møller BL and Scheller HV (1992) A membrane-bound monoheme cytochrome c 551 of a novel type is the immediate electron donor to P840 of the Chlorobium vibrioforme photosynthetic reaction center complexes. J Biol Chem 267: 21139–21145
Otte SCM, van derHeiden JC, Pfennig N and Amesz J (1991) A comparative study of the optical characteristics of intact cells of photosynthetic green sulfur bacteria containing bacteriochlorophyll c, d or e. Photosynth Res 28: 159–169
Swarthoff T and Amesz J (1979) Photochemically active pigment-protein complexes from the green photosynthetic bacterium Prosthecochloris aestuarii. Biochim Biophys Acta 548: 427–432
Swarthoff T, van derVeek-Horsley KM and Amesz J (1981) The primary charge separation, cytochrome oxidation and triplet formation in preparations from the green photosynthetic bacterium Prosthecochloris aestuarii. Biochim Biophys Acta 635: 1–12
van deMeent EJ, Kobayashi M, Erkelens C, vanVeelen PA, Amesz J and Watanabe T (1991) Identification of 81-hydroxychlorophyll a as a functional reaction center pigment in heliobacteria. Biochim Biophys Acta 1058: 356–362
van deMeent EJ, Kobayashi M, Erkelens C, vanVeelen PA, Otte SCM, Inoue K, Watanabe T and Amesz J (1992) The nature of the primary electron acceptor in green sulfur bacteria. Biochim Biophys Acta 1102: 371–378
van Dorssen RJ (1988) Structure and function of light-harvesting complexes in photosynthesis. Doctoral thesis, Leiden University
vanDorssen RJ, Gerola PD, Olson JM and Amesz J (1986) Optical and structural properties of chlorosomes of the photosynthetic green sulfur bacterium Chlorobium limicola. Biochim Biophys Acta 848: 77–82
Vasmel H, Swarthoff T, Kramer HJM and Amesz J. (1983) Isolation and properties of a pigment-protein complex associated with the reaction center of the green photosynthetic sulfur bacterium Prosthecochloris aestuarii. Biochim Biophys Acta 725: 361–367
Wang J, Brune DC and Blankenship RE (1990) Effects of oxidants and reductants on the efficiency of excitation transfer in green photosynthetic bacteria. Biochim Biophys Acta 1015: 457–463
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Francke, C., Otte, S.C.M., Miller, M. et al. Energy transfer from carotenoid and FMO-protein in subcellular preparations from green sulfur bacteria. Spectroscopic characterization of an FMO-reaction center core complex at low temperature. Photosynth Res 50, 71–77 (1996). https://doi.org/10.1007/BF00018222
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DOI: https://doi.org/10.1007/BF00018222