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Electron transport to oxygen mitigates against the photoinactivation of Photosystem II in vivo

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Abstract

The role of electron transport to O2 in mitigating against photoinactivation of Photosystem (PS) II was investigated in leaves of pea (Pisum sativum L.) grown in moderate light (250 μmol m−2 s−1). During short-term illumination, the electron flux at PS II and non-radiative dissipation of absorbed quanta, calculated from chlorophyll fluorescence quenching, increased with increasing O2 concentration at each light regime tested. The photoinactivation of PS II in pea leaves was monitored by the oxygen yield per repetitive flash as a function of photon exposure (mol photons m−2). The number of functional PS II complexes decreased nonlinearly with increasing photon exposure, with greater photoinactivation of PS II at a lower O2 concentration. The results suggest that electron transport to O2, via the twin processes of oxygenase photorespiration and the Mehler reaction, mitigates against the photoinactivation of PS II in vivo, through both utilization of photons in electron transport and increased nonradiative dissipation of excitation. Photoprotection via electron transport to O2 in vivo is a useful addition to the large extent of photoprotection mediated by carbon-assimilatory electron transport in 1.1% CO2 alone.

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Abbreviations

Fm, Fo, Fv-:

maximal, initial (corresponding to open PS II traps) and variable chlorophyll fluorescence yield, respectively

NPQ-:

non-photochemical quenching

PS-:

photosystem

QA-:

primary quinone acceptor

qP-:

photochemical quenching coefficient

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Author notes

  1. Youn-II Park

    Present address: Department of Plant Physiology, University of Umeå, S 90187, Umeå, Sweden

Authors and Affiliations

  1. Photobioenergetics Group, Research School of Biological Sciences, Australian National University, GPO Box 475, 260I, Canberra, ACT, Australia

    Youn-II Park, Wah Soon Chow, C. Barry Osmond & Jan M Anderson

  2. Cooperative Research Center for Plant Science and CSIRO, Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia

    Wah Soon Chow & Jan M Anderson

Authors
  1. Youn-II Park
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  2. Wah Soon Chow
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  3. C. Barry Osmond
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  4. Jan M Anderson
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Park, YI., Chow, W.S., Osmond, C.B. et al. Electron transport to oxygen mitigates against the photoinactivation of Photosystem II in vivo . Photosynth Res 50, 23–32 (1996). https://doi.org/10.1007/BF00018218

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  • DOI: https://doi.org/10.1007/BF00018218

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