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Chlorophyll fluorescence transients in a barley mutant lacking Photosystem I

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Abstract

We have compared the properties of a mutant of barley lacking Photosystem I (viridis-zb 63) with the corresponding wild type using modulated fluorescence measurements. The mutant showed two unexpected characteristics. Firstly, there was a slow decline in the fluorescence signal in the light which was dependent on the presence of O2 at concentrations similar to that in air; 2% O2 in N2 had no effect. The observed decline was mainly due to an increase in the non-photochemical quenching. Secondly, in the absence of O2, saturating light pulses caused a pronounced transient decrease in the fluorescence signal; a similar effect could also be observed in wild type plants when neither CO2 nor O2 was present.

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Abbreviations

PPFD-:

photosynthetic photon flux density

qN-:

non-photochemical quenching of chlorophyll fluorescence

qp-:

photochemical quenching of chlorophyll fluorescence

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Authors and Affiliations

  1. Institute of Biochemistry, Odense University, Campusvej 55, DK 5230, Odense M, Denmark

    René K. Juhler, Mette Miller & Raymond P. Cox

  2. Carlsberg Laboratory, Department of Physiology, Gamle Carlsbergvej 10, DK 2500, Copenhagen, Valby, Denmark

    David Simpson

Authors
  1. René K. Juhler
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  2. Mette Miller
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  3. David Simpson
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  4. Raymond P. Cox
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Juhler, R.K., Miller, M., Simpson, D. et al. Chlorophyll fluorescence transients in a barley mutant lacking Photosystem I. Photosynth Res 35, 305–310 (1993). https://doi.org/10.1007/BF00016561

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  • DOI: https://doi.org/10.1007/BF00016561

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