Abstract
Callus induction of the agarophyte marine alga Gracilaria verrucosa was obtained on modified ASP6 F2 media. The formation of callus from axenic explants depended on the addition of glycerol (0.217 mol 1−1) and plant growth regulators (2.0 mg l−1 IPA, 2.0 mg l−1 IAA, and 1.0 mg l−1 2iP). Glycerol alone, did not induce callus. The addition of 5.0 mg l−1 DPU (Diphenylurea) caused callus induction on the whole surface of the explants of Gracilaria verrucosa. Different culture-media, carbon sources and phytohormones were tested. The application of floridoside (2-O-D-glycerol-alpha-D-galactopyranoside), the main product of photosynthesis of the red algae, did not promote growth and callus induction.
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References
Allen, M. B., 1959. Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch. Mikrobiol. 32: 270–277.
Amano, H. & H. Noda, 1987. Effect of nitrogenous fertilizers on the recovery of discoloured fronds of Porphyra yezoensis. Bot. Mar. 30: 467–473.
Angier, H., 1976. Les hormones des algues. Etat actuel des connaissances. III.-Rôle des hormones dans les modalités de croissance et de développement des thalles. Bot. Mar. 19: 351–377.
Bergh, Ø., K. Y. Børsheim, G. Bratbak & M. Heldal, 1989. High abundance of viruses found in aquatic environments. Nature 340: 467–468.
Bradley, P. M., 1991. Plant hormones do have a role in controlling growth and development of algae. J. Phycol. 27: 317–321.
Bradley, P. M., D. P. Cheney & N. Saga, 1988. One step antibiotic disk method for obtaining axenic cultures of multicellular marine algae. Plant Cell, Tissue and Organ Culture 12: 55–60.
Bradley, P. M. & D. P. Cheney, 1990. Some effects of plant growth regulators on tissue cultures of the marine red alga Agardhiella subulata (Gigartinales, Rhodophyta). Hydrobiologia 204/205: 353–360.
De Fossard, R. A. & H. de Fossard, 1988. Coping with microbial contaminants and other matters in a small commercial micropropagation laboratory. Acta Hort. 225: 167–176.
Dunlap, J. R. & K. M. Robacker, 1988. Nutrient salts promote light-induced degradation of indole-3-acetic acid in tissue culture media. Plant Physiol. 88: 379–382.
Evans, L. V. & A. J. Trewavas, 1991. Is algal development controlled by plant growth substances? J. Phycol. 27: 322–326.
Fries, L., 1973. Requirements for organic substances in seaweeds. Bot. Mar. 16: 19–31.
Fries, L., 1977. Growth regulating effects of phenylacetic acid and p-hydroxyphenylacetic acid on Fucus spiralis L. (Phaeophyceae, Fucales) in axenic culture. Phycologia 16: 451–455.
Fries, L., 1991. Formation of filaments and single cells by the activity of meristoderm of axenic Ascophyllum nodosum (Fucaceae, Phaeophyta). Phycologia 30: 310–313.
Garcia-Reina, G., J. L. Gomez-Pinchetti, D. R. Robledo & P. Sosa, 1991. Actual, potential and speculative applications of seaweed cellular biotechnology: some specific comments on Gelidium. Hydrobiologia 221: 181–194.
Gusev, M. V., A. H. Tambiev, N. N. Kirikova, N. N. Shelyastina & R. R. Aslanyan, 1987. Callus formation in seven species of agarophyte marine algae. Mar. Biol. 95: 593–597.
Jacobs, W. P., 1985. Are angiosperm hormones present in, and used as hormones by, algae? In M. Bopp (ed.), Plant growth substances 1985. Springer-Verlag, Berlin/ Heidelberg: 249–256.
Kawashima, Y. & H. Tokuda, 1990. Callus formation in Ecklonia cava Kjellman (Laminariales, Phaeophyta). Hydrobiologia 204/205: 375–380.
Kloareg, B., X.-W. Liu, C. Boyen, Y. le Gall, X.-D. Zha & P. Potin, 1989. Perspectives d'applications des biotechnologies végétales a la micropropagation et a l'amélioration gé-nétique des algues marines d'intérêt économique. Oceanis 15: 649–659.
Lawlor, H. J., J. A. McComb & M. A. Borowitzka, 1989. Tissue culture of Ecklonia radiata (Phaeophyceae, Laminariales): effects on growth of light, organic carbon source and vitamins. J. appl. Phycol. 1: 105–112.
Melkonian, M., 1991. Systematics and evolution of the algae. Progr. Bot. 52: 271–307.
Mooney, P. A. &L. van Staden, 1986. Algae and cytokinins. J. Plant Physiol. 123: 1–21.
Mooney, P. A. & L. van Staden, 1987. Tentative identification of cytokinins in Sargassum heterophyllum (Phaeophyceae). Bot. Mar. 30: 323–325.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Physiol. Pl. 15: 473–497.
Nakamura, T., 1974. Production of agar. Japan Patent (Kokai) 74–101561: 441–445.
Polne-Fuller, M. & A. Gibor, 1987. Calluses and callus-like growth in seaweeds: Induction and culture. Hydrobiologia 151/152: 131–138.
Robaina, R. R., G. Garcia-Reina & A. Luque, 1990. The effects of the physical characteristics of the culture medium on the development of red seaweeds in tissue culture. Hydrobiologia 204/205: 137–142.
Robaina, R. R., P. Garcia-Jimenez & A. Luque, 1992. The growth pattern and structure of callus from the Red Alga Laurencia sp. (Rhodophyta, Ceramiales) compared to shoot regeneration. Bot. Mar. 35: 267–272.
Shantz, E. M. & F. C. Steward, 1955. The identification of compound A from Coconut Milk as 1,3-Diphenylurea. J. am. Chem. Soc. 77: 6351–6353.
Starr, R. C., 1978. The culture collection of algae at the university of Texas at Austin. Additions to the UTEX collection since 1978. J. Phycol. 14 suppl.: 47–100.
Thorpe, T. A., 1981. Plant tissue culture, methods and application in agriculture. Academic Press.
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Kaczyna, F., Megnet, R. The effects of glycerol and plant growth regulators on Gracilaria verrucosa (Gigartinales, Rhodophyceae). Hydrobiologia 268, 57–64 (1993). https://doi.org/10.1007/BF00005741
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DOI: https://doi.org/10.1007/BF00005741