Abstract
Cholecalciferol (Vitamin D3) increased plasma inorganic phosphate concentration in American eels,Anguilla rostrata, in a dose-dependent fashion. This response was more marked in phosphate loaded fish. In control as well as phosphate loaded eels the hyperphosphatemic response to D3 was associated with a sharp reduction in renal phosphate clearance relative to14C-polyethelene glycol (PEG) clearance. Glomerular filtration and urine flow rates were not affected by D3. As renal phosphate clearance, even in phosphate loaded eels, never significantly exceeded that of PEG, it is suggested that D3 reduced the relative clearance rate of phosphate by increasing renal phosphate reabsorption rather than by reducing the tubular secretion of phosphate.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References cited
Bertin, L. 1956. Eels, A. Biological Study. Cleaner-Hume, London.
Bijvoet, O.L.M. 1980. Indices for the measurement of the renal handling of phosphate.In Renal Handling of Phosphate. pp. 1–37. Edited by S.G. Massry and H. Fleisch. Plenum Medical Book Company, New York.
Bijvoet, O.L.M. and Reitsma, P.H. 1977. Phylogeny of renal phosphate transport in the vertebrates.In Phosphate Metabolism. Advances in Experimental Medicine and Biology. Vol. 81. pp. 41–53. Edited by S.G. Massry and E. Ritz. Plenum Press, New York.
Bonjour, J.P. and Caverzasio, J. 1984. Phosphate transport in the kidney. Rev. Physiol. Biochem. Pharmacol. 100: 161–214.
Bornancin, M., De Renzis, G., and Maetz, J. 1977. Branchial Cl transport, anion-stimulated ATPase and acid-base balance inAnguilla anguilla adapted to freshwater: effects of hyperoxia. J. Comp. Physiol. 117: 313–322.
Chartier, M.M., Millet, C., Martelly, E., Lopez, E. and Warrot, S. 1979. Stimulation par la vitamine D3 et le 1,25-dihydroxyvitamine D3 de l'absorption intestinale du calcium chez l'anguille (Anguilla anguilla L.). J. Physiol., Paris 75: 275–282.
Dave, G., Johansson-Sjobeck, M-L., Larsson, Å., Lewander, K. and Lidman, U. 1975. Metabolic and hematological effects of starvation in the European eel,Anguilla anguilla L. I. Carbohydrate, lipid, protein and inorganic ion metabolism. Comp. Biochem. Physiol. 52A: 423–430.
Dacke, C.G. 1979. Calcium Regulation in Submammalian Vertebrates. Academic Press, London.
Fenwick, J.C. 1976. Effect of stanniectomy on calcium activated adenosinetriphosphatase activity in the gills of fresh water adapted North American eels,Anguilla rostrata LeSueur. Gen. Comp. Endocrinol. 29: 383–387.
Fenwick, J.C., Smith, K., Smith, J. and Flik, G. 1984. Effect of various vitamin D analogs on plasma calcium and phosphorus and intestinal calcium absorption in fed and unfed American eels,Anguilla rostrata. Gen. Comp. Endocrinol. 55: 398–404.
Gmaj, P. and Murer, H. 1986. Cellular mechanisms of inorganic phosphate transport in kidney. Physiol. Rev. 66: 36–70.
Hyde, D.A. and Perry, S.F. 1987. Acid-base and ionic regulation in the American eel (Anguilla rostrata) during and after prolonged aerial exposure: branchial and renal adjustments. J. Exp. Biol. 133: 429–447.
Karnaky, K.J., Jr. 1986. Structure and function of the chloride cell ofFundulus heteroclitus and other teleosts. Am. Zool. 26: 209–224.
Lopez, E., MacIntyre, I., Martelly, E., Lallier, F., and Vidal, B. 1980. Paradoxical effect of 1,25 dihydroxycholecalciferol on osteoblastic and osteoclastic activity in the skeleton of the eel,Anguilla anguilla L. Calcif. Tiss. Int. 32: 83–87.
Lopez, E., Peignoux-Deville, J., Lallier, F., Colston, K.W. and MacIntyre, I. 1977. Response of bone metabolism in the eel (Anguilla anguilla) to injections of 1,25-dihydroxyvitamin D3. Calcif. Tiss. Res. Suppl. 22: 19–23.
Love, R.M. 1970. The Chemical Biology of Fishes. pp. 222–257. Academic Press New York.
MacIntyre, I., Colston, K.W., Evans, I.M.A., Lopez, E., MacAuley, S.J., Peignoux-Deville, J., Spanos, E. and Szelke, M. 1976. Regulation of vitamin D: An evolutionary view. Clin. Endocrinol. Suppl. 5: 85s–95s.
Mizgala, C.L. and Quamme, G.A. 1985. Renal handling of phosphate. Physiol. Rev. 65: 431–466.
Nakamura, Y. 1985.In vitro absorption of inorganic phosphate and other electrolytes in the carp intestine. Comp. Biochem. Physiol. 80A: 17–20.
Ogino, C. and Takeda, H. 1976. Mineral requirement in fish. III. Calcium and phosphorus requirements in carp. Bull. Jap. Soc. Sci. Fish. 42: 71–75.
Pautard, F. 1961. Calcium, phosphorus and the origin of backbones. New Scientist 260: 364–366.
Schmidt-Nielsen, B. and Renfro, L. 1975. Kidney function of the American eelAnguilla rostrata. Am. J. Physiol. 228: 420–431.
Steel, R.G.D. and Torrie, J.H. 1960. Principles and Procedures of Statistics. McGraw-Hill, New York.
Swarup, S., Norman, A.W., Srivastav, A.K. and Srivastav, S.P. 1984. Dose-dependent vitamin D3 and 1,25-dihydroxyvitamin D3-induced hypercalcemia and hyperphosphatemia in male catfishClarias batrachus. Comp. Biochem. Physiol. 78B: 553–555.
Wheatly, M.G., Höbe, H. and Wood, C.M. 1984. The mechanisms of acid-base and ionoregulation in the freshwater rainbow trout during environmental hyperoxia and subsequent normoxia. II. The role of the kidney. Respir. Physiol. 55: 155–173.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fenwick, J.C., Vermette, M.G. Vitamin D3 and the renal handling of phosphate in American eels. Fish Physiol Biochem 7, 351–358 (1989). https://doi.org/10.1007/BF00004728
Issue Date:
DOI: https://doi.org/10.1007/BF00004728