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Paradoxical effect of 1.25 dihydroxycholecalciferol on osteoblastic and osteoclastic activity in the skeleton of the eelAnguilla anguilla L

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Summary

Female mature eels (300 to 500 g) received one intraperitoneal injection of 1.25 (OH)2D3 (10 µg). Their vertebral bone was compared, 8 h and 24 h after the injection, with vertebral bone of control mature female eels receiving solvent alone (ethanol). Sexual maturation in female eels induces a bone decalcification with hypercalcaemia and hyperphosphataemia. The control eels showed marked osteoclastic resorption and osteocytic osteolysis and the degree of mineralization of the intercellular substance decreased. Injection of 1.25 (OH)2D3 into these female mature eels provoked as early as 8 h : 1) an increase in hypercalcaemia and hyperphosphataemia ; 2) a major conversion of lining cells to osteoblasts and a stimulation of osteoblastic activity with new bone formation ; 3) diminished osteoclastic resorption without changing osteocytic osteolysis or bone matrix mineralization.

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References

  1. Fleschman, W.: Comparative physiology of the thyroid gland, Q. Rev. Biol. 22: 119–140, 1947.

    Article  Google Scholar 

  2. Pang, P.K.T., Pang, R.K., Sawyer, W.H.: Environmental calcium and the sensitivity of killifish (Fundulus heteroclitus) in bioassays for the hypocalcemic responses to Stannius corpuscles from killifish and cod (Gadus morhua), Endocrinology 94: 548–555, 1974.

    Article  CAS  PubMed  Google Scholar 

  3. Peignoux-Deville, J., Milet, C., Martelly, E.: Effects de l'ablation du corps ultimobranchial et de la perfusion de calcitonine sur les flux de calcium au niveau des branchies de l'anguille (Anguilla anguilla L.), Anns. Biol. anim. Biochim. Biophys. 18: 119–126, 1978.

    CAS  Google Scholar 

  4. Milet, C., Peignoux-Deville, J., Martelly, E.: Gill calcium fluxes in the eel,Anguilla anguilla (L.). Effects of Stannius corpuscles and ultimobranchial body, Comp. Biochem. Physiol. 63A: 63–70, 1979.

    Article  CAS  Google Scholar 

  5. Lopez, E.: L'os cellulaire d'un poisson téléostéenAnguilla anguilla L. II. Action de l'ablation des corpuscules de Stannius, Z. Zellforsch. mikrosk. Anat. 109: 566–572, 1970.

    Article  CAS  PubMed  Google Scholar 

  6. Lopez, E., Peignoux-Deville, J., Lallier F., Martelly, E., Milet, C.: Effects of calcitonin and ultimobranchialectomy (UBX) on calcium and bone metabolism in the eel,Anguilla anguilla L., Calcif. Tiss. Res. 20: 173–186, 1976.

    CAS  Google Scholar 

  7. Hillyard, 1977, private communication (unpublished).

  8. Hay, A.W.H., Watson, G.: The plasma transport proteins of 25 hydroxycholecalciferol in fish, amphibians, reptiles and birds, Comp. Biochem. Physiol. 53B: 167–172, 1976.

    Google Scholar 

  9. Hay, A.W.H., Watson, G.: Binding properties of serum vit. D transport proteins in vertebrates for 24R,25-dihydrocholecalciferol and 24S,25-dihydrocholecalciferolin vitro, Comp. Biochem. Physiol. 58: 43–48, 1977.

    CAS  Google Scholar 

  10. Henry, H., Norman, A.W.: Presence of renal 25-hydroxyvitamin D-1-hydroxylase in species of all vertebrate classes, Comp. Biochem. Physiol. 50B: 431–434, 1975.

    Google Scholar 

  11. Brown, D.J., Milet, C., Lopez, E., 1979, (unpublished data).

  12. Mac Intyre, I., Colston, K.W., Evans, I.M.A., Lopez, E., Mac Auley, S.J., Peignoux-Deville, J., Spanos, E., Szelke, M.: Regulation of vitamin D : an evolutionary view, Clin. Endocr. 5 (suppl.): 85S-95S, 1976.

    Article  Google Scholar 

  13. Lopez, E., Peignoux-Deville, J., Lallier, F., Colston, K.W., Mac Intyre, I.: Responses of bone metabolism in the eel (Anguilla anguilla) to injection of 1.25 (OH)2D3, Calcif. Tiss. Res. suppl. 22: 19–23, 1977.

    Article  Google Scholar 

  14. Lopez, E. (Unpublished data).

  15. Lopez, E., Martelly, E.: L'os cellulaire d'un poisson téléostéen,Anguilla anguilla L. III. Etude histologique et histophysique au cours de la maturation provoquée par injections d'extrait hypophysaire de carpe, Z. Zellforsch. 117: 176–190, 1971.

    Article  CAS  PubMed  Google Scholar 

  16. Fontaine, M., Bertrand, E., Lopez, E., Callamand, O.: Sur la maturation des organes génitaux de l'anguille femelleAnguilla anguilla L. et l'émission spontanée des oeufs en aquarium, C. R. Acad. Sci. (Paris) 259: 2907–2910, 1964.

    CAS  Google Scholar 

  17. Jowsey, J., Kelly, P.J., Riggs, B.L., Bianco, A.J., Scholtz, D.A., Gershon-Cohen, J.: Quantitative microradiographic studies of hormonal and osteoporotic bone, J. Bone Jt. Surg. 47: 785–806, 1965.

    CAS  Google Scholar 

  18. Frost, H.M., Villanueva, A.R., Roth, H.: Qualitative method for measuring osteoclastic activity. Henry Ford Hosp. Bull. 10:217–228, 1962.

    CAS  Google Scholar 

  19. Bordier, Ph., Matrajt, H., Miravet, L., Hioco, D.: Mesure histologique de la masse et de la résorption des travées osseuses. Path. et Biol. 12: 1238–1243, 1964.

    CAS  Google Scholar 

  20. Baud, C.A.: Radiographies osseuses quantitatives, Praxis 46: 329–331, 1957.

    CAS  PubMed  Google Scholar 

  21. Lopez, E.: L'os cellulaire d'un poisson téléostéen (Anguilla anguilla L.). I. Etude histocytologique et histophysique, Z. Zellforsch 109: 552–565, 1970.

    Article  CAS  PubMed  Google Scholar 

  22. Fiske, C.H., Subbarow, Y.: The colorimetric determination of phosphorus, J. Biol. Chem. 66: 375–400, 1975.

    Google Scholar 

  23. Lopez, E., Baud, C.A., Vidal, B., Lallier, F. : Osteoclastic acticity of lining-cells in bone of fish. In preparation.

  24. Lopez, E. : Etude morphologique et physiologique de l'os cellulaire des poissons téléostéens (Thèse de Doctorat d'Etat, 1972). Mémoires du Muséum national d'Histoire naturelle, série A, Zoologie tome LXXX, 1973.

  25. Lopez, E.: Demonstration of several forms of decalcification in bone of the teleost fishAnguilla anguilla L., Calcif. Tiss. Res. 4 (suppl.): 83, 1970.

    Article  Google Scholar 

  26. Chartier, M.M., Milet, C., Martelly, E., Warrot, S. : Stimulation par la vitamine D3 et le 1,25-dihydroxyvitamine D3 de l'absorption intestinale du calcium chez l'anguille (Anguilla anguilla L.), J. Physiol. (Paris) 75: 1979 (in press).

  27. Frolik, C.A., De Luca, H.F.: Metabolism of 1.25 dihydroxycholecalciferol in the rat, J. Clin. Invest. 51: 2900–2906, 1972.

    Article  CAS  PubMed  Google Scholar 

  28. Wong, R.G., Myrtle, J.F., Tsai, H.C., Norman, A.W.: Studies on calciferol metabolism, J. Biol. Chem. 247: 5728–5735, 1972.

    CAS  PubMed  Google Scholar 

  29. Rasmussen, H., Bordier, P.: Vitamin D and bone, Metab. Bone Dis. et Rel. Res. 1: 7–13, 1978.

    Article  CAS  Google Scholar 

  30. Chung-Ching Liu, D., Baylink, J., Wergedal, J.: Vitamin D. Enhanced osteoclastic bone resorption at vascular canals, Endo. 95: 1011–1018, 1974.

    Google Scholar 

  31. Anderson, H.C., Matsuzawa, T., Sajdera, S.W., Ali, S.Y.: Membranous particles in calcifying cartilage matrix, Trans. N.Y. Acad. Sci. 32: 619–630, 1970.

    CAS  PubMed  Google Scholar 

  32. Lopez, E., Baud, C.A., Boivin, G., Lallier, F.: Etude ultrastructurale, chez un poisson téléostéen, l'anguille (Anguilla anguilla L.), des processus de minéralisation dans les cas d'une ossification périchondrale de l'arc branchial et d'une apposition secondaire dans l'os vertebral, Anns. Biol. anim. Biochem. Biophys. 18: 105–117, 1978.

    CAS  Google Scholar 

  33. Weiss, R.E., Watabe, N.: Studies on the biology of fish bone. III. Ultrastructure of osteogenesis and resorption in osteocytic (cellular) and anosteocytic (acellular) bones, Calcif. Tissue Int. 28: 43–56, 1979.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Physiologie générale et comparée du Muséum national d'Histoire naturelle et Laboratoire d'Endocrinologie comparée associé au C.N.R.S., 7, rue Cuvier, 75231, Paris cédex 05, France

    E. Lopez, I. Mac Intyre, E. Martelly, F. Lallier & B. Vidal

  2. Endocrine Unit, Royal Postgraduate Medical School, Du Cane Road, W12 0HS, London, England

    I. Mac Intyre

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  1. E. Lopez
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  2. I. Mac Intyre
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  4. F. Lallier
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  5. B. Vidal
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Lopez, E., Mac Intyre, I., Martelly, E. et al. Paradoxical effect of 1.25 dihydroxycholecalciferol on osteoblastic and osteoclastic activity in the skeleton of the eelAnguilla anguilla L . Calcif Tissue Int 32, 83–87 (1980). https://doi.org/10.1007/BF02408525

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