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Industrial Production and Optimization of Microbial Enzymes

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Microbial Enzymes: Roles and Applications in Industries

Part of the book series: Microorganisms for Sustainability ((MICRO,volume 11))

Abstract

Microbial enzymes are the biological catalysts due to their ability to favor reactions more quickly and more efficiently. Various enzymes are produced by microorganisms for industrial uses. They must possess the desired properties like diversified functionality, and stability over pH and temperature ranges. Microorganisms have to produce extracellular enzymes in higher amounts and the produced enzymes have to be safe, stable, and more active. Microbial enzymes with the desired properties can be produced by optimizing fermentation conditions. To make the fermentation cost effective, the utilization of low-cost substrates such as agricultural and spent residues for microbial enzyme production is necessary. Some industrial enzymes used together for the same purpose (like amylase, lipase, and protease used in detergent formulation) are co-produced in a single fermentation to reduce the cost and to maintain the enzyme stability. In addition, for some microorganisms, recombinant DNA technology is used as an alternative strategy for overproducing huge amounts of microbial enzymes with improved substrate specificity and stability. Furthermore, novel techniques like genetic fusion of coding open reading frames or connection of proteins in a posttranslational process are used to manufacture the fused industrial enzymes having combined properties of their parental molecules. The public and private companies have thus to work together with academicians and researchers in order to increase production of microbial enzymes needed by the industries. This chapter reviews the production of industrial enzymes and optimization of culture and fermentation conditions in order to increase production of microbial enzymes in huge amounts.

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Niyonzima, F.N., Veena, S.M., More, S.S. (2020). Industrial Production and Optimization of Microbial Enzymes. In: Arora, N., Mishra, J., Mishra, V. (eds) Microbial Enzymes: Roles and Applications in Industries. Microorganisms for Sustainability, vol 11. Springer, Singapore. https://doi.org/10.1007/978-981-15-1710-5_5

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