Abstract
A continuous flow centrifuge has been used as an external perfusion system for high density culture of hybridomas and HeLa cells in stirred tank bioreactors. During continuous runs of two weeks no clogging of cells inside the rotor could be observed. More than 99.99 % of the cells could be retained and recirculated into the bioreactor. Cell viability and population doubling time were not influenced by the centrifugation process or circulation by peristaltic pumps. The cell specific productivity of the hybridoma line was significantly increased compared to batch culture.
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References
Blasey, H.D. and Jäger, V. (1991) ‘Strategies to Increase the Efficiency of Membrane Aerated and Perfused Animal Cell Bioreactors by an Improved Medium Perfusion’, in Sasaki, R. and Ikura, K. (eds.), Animal Cell Culture and Production of Biologicals, Kluwer Acad. Pub., Dordrecht, pp. 61–73
Büntemeyer, H., Bödeker, B.G.D. and Lehmann, J. (1987) ‘Membrane-Stirrer-Reactor for Bubble Free Aeration and Perfusion’, in Spier, R.E. and Griffiths, J.B. (eds.), Modem Approaches to Animal Cell Technology, Butterworths, Sevenoaks, pp. 411–419
Hamamoto, K., Ishimaru, K. and Tokashiki, M. (1989) ‘Perfusion Culture of Hybridoma Cells Using a Centrifuge to Separate Cells from Culture Mixture’, J. Ferment. Bioeng. 67, 190–194
Himmelfarb, P., Thayer, P.S. and Martin, H.S. (1969) ‘Spin Filter Culture: The Propagation of Mammalian Cells in Suspension’, Science 164, 555–557
Jäger, V., Lehmann, J. and Friedl, P. (1988) ‘Serum-Free Growth Medium for the Cultivation of a Wide Spectrum of Mammalian Cells in Stirred Bioreactors’, Cytotechnology 1, 319–329
Lehmann, J., Piehl, G.W. and Schulz, R. (1987) ‘Bubble Free Cell Culture Aeration with Porous Moving Membranes’, Develop, biol. Standard. 66, 227–240
Tokashiki, M., Hamamoto, K., Takazawa, Y. and Ichikawa, Y. (1988) ‘High Density Culture of Mouse-Human Hybridoma Cells Using a New Perfusion Culture Vessel’, Kagaku Kogaku Ronbunshu 14, 337–341
Tokashiki, M., Arai, T., Hamamoto, K. and Ishimaru, K. (1990) ‘High Density Culture of Hybridoma Cells Using a Perfusion Culture Vessel with an External Centrifuge’, Cytotechnology 3, 239–244
Tokashiki, M. and Arai, T. (1991) ‘High Density Perfusion Culture of Hybridoma Cells Using a Perfiision Culture Apparatus with Multi-Settling Zones’, in Spier, R.E., Griffiths, J.B. and Meignier, B. (eds.), Production of Biologicals from Animal Cells in Culture, Butterworth-Heinemann, Oxford, pp. 467–469
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© 1992 Springer Science+Business Media Dordrecht
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Jäger, V. (1992). High Density Perfusion Culture of Animal Cells Using a Novel Continuous Flow Centrifuge. In: Murakami, H., Shirahata, S., Tachibana, H. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2844-5_29
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DOI: https://doi.org/10.1007/978-94-011-2844-5_29
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5263-4
Online ISBN: 978-94-011-2844-5
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