Abstract
Published studies on the tissue culture of Dianthus include many references to the problem of vitrification [e.g. 16,17,18,28,29,30] or hyperhydration [7]. Indeed, Dianthus is often chosen as a model for research on vitrification [e.g. see 13, 14, 20,]. These and other studies on vitrification have implicated both media factors and aspects of the in vitro gaseous atmosphere in this phenomenon. Keevers and coworkers [13] have implicated ethylene accumulation in the culture vessel as a contributory factor in vitrification and many of the strategies employed to reduce vitrification in cultures consciously, or subliminally, reduce ethylene accumulation or facilitate its escape [5, 10, 24 29].The water vapour pressure in the vessel has been implicated in poor establishment of micro plants [9, 22].
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Abbreviations
- MVTR:
-
Moisture Vapour Transmission Rate
- LDPE:
-
Low Density Polyethylene
- PVC:
-
Polyvinylchloride
- RH:
-
Relative humidity
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Cassells, A.C., Roche, T.D. (1994). The influence of the gas permeability of the vessel lid and growth-room light intensity on the characteristics of Dianthus microplants in vitro and ex vitrum . In: Lumsden, P.J., Nicholas, J.R., Davies, W.J. (eds) Physiology, Growth and Development of Plants in Culture. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0790-7_19
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DOI: https://doi.org/10.1007/978-94-011-0790-7_19
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