Skip to main content
SpringerLink
Log in

Somatic embryogenesis in olive (Olea europaea L.)

  • Chapter
Somatic Embryogenesis in Woody Plants

Part of the book series: Forestry Sciences ((FOSC,volume 44-46))

Abstract

The olive, which originated in the eastern Mediterranean basin, belongs to the Oleaceae family and comprises about 30 genera including various garden and economical plants (Knoblauch, 1895; Ciferri, 1942; Taylor, 1945; Johnson, 1957; Mazzolani and Altamura Betti, 1979, 1980; Altamura Betti et al., 1985). Sax and Abbe (1932) found that six genera of two tribes belonging to the sub-family Oleoideae had the basic haploid chromosome number of 23 (2n = 2x = 46). Other than in Mediterranean countries, subspecies are also found in western Africa, Tanzania, the Canary and Azore Islands, South Africa, Mauritius, and Mascarene Comore. During the last 30–40 years, cultivated olives have been introduced into the USA, Australia, South Africa, China, Korea and other Asiatic countries. Most cultivated olives belong to Olea europaea L. which comprises many groups of forms; up to 2600 different cultivars have been recorded, although many of these might be ecotypes. These groups may have originated by mutation and hybridization (Chevalier, 1948) and in addition, as indicated by palaeontological studies,

Table 1 Summary of work carried out in somatic embryogenesis on olive. The best basal media and hormone concentration are reported in brackets, both in induction () and regeneration [ ] media
Full size table

MS — Murashige and Skoog (1962); NAA — naphthaleneacetic acid; BA — 6-benzylaminopurine; IBA — indolebutyric acid; SH — Schenk and Hildebrand (1972); 2iP — 6 dimethylaminopurine; OMc — olive medium (OM; Rugini, 1984) in which macroelements were replaced with those of Bourgin and Nitsch (1967) with addition of 1 gm/1 casein hydrolysate and omitting glutamine.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Altamura Betti, M.M., L. Altamura and G. Mazzolani, 1985. Elements for the revision of the genus Olea (Tourn.) L. IV. The taxa present in Oceania which can be ascribed to Olea and allied genera. Ann. Bot. 43: 45–52.

    Google Scholar 

  • Bao, Z.-H., Y.-F. Ma, J.-F. Liu, K.-J. Wang, P.-F. Zhang, D.-X. Ni and W.-Q Yang, 1980. Induction of plantets from the hypocotyl of Olea europaea L. in vitro. Acta Bot. Sinica 2: 96–97.

    Google Scholar 

  • Bartolini S., A. Minnocci and C. Vitagliano, 1991. Morphological studies on pollen in some clones of olive “Leccino” cultivar. Agr. Med. 122: 282–296.

    Google Scholar 

  • Bellini, E. and E. Giordani, 1992. Miglioramento genetico dell’olivo: comportamento ereditario di alcuni caratteri. II. Contributo. In: Atti Qualità Olio Extravergine di Oliva, Firenze, 1–3 December, 1982, pp. 273–275.

    Google Scholar 

  • Berenguer, A.G. 1978. Seleccion clonal en Olivo (Olea europaea L.). Olea 1: 7–15.

    Google Scholar 

  • Bogani, P., R. Petruccelli, L. Polsinelli and G. Roselli, 1993. Identification of olive tree cultivars by using random amplified polimorphic DNA. In: The 2th International Symposium on Olive Growing, Jerusalem, September 6–10, 1993, p. 22.

    Google Scholar 

  • Bourgin, J.P. and J.P. Nitsch, 1967. Obtention de Nicotiana haploides à partir d’étamines cultivées in vitro. Ann. Physiol. Veg. 9: 377–383.

    Google Scholar 

  • Cañas, L.A. and A. Benbadis, 1988. Plant regeneration from cotyledon fragments of the olive tree (Olea europaea L.). Plant Sci. 54: 65–74.

    Article  Google Scholar 

  • Cañas, L.A., A.M. Wyssmann and M.C. Benbadis, 1987. Isolation, culture and division of olive (Olea europaea L) protoplasts. Plant Cell Rep. 5: 369–371.

    Article  Google Scholar 

  • Chevalier, A., 1948. L’origine de l’Olivier cultivé et ses variations. Revue Botanique Appliquée 303-304: 1–25.

    Google Scholar 

  • Ciferri, R., 1942. Recenti progressi degli Studi botanico-agrari sull’olivo. Convegno di Studi Olivicoli. Regia Accademia Georgofili, Firenze, pp. 49–95.

    Google Scholar 

  • Durante, M., R. Petruccelli, G. Bartolini and R. Bernardi, 1992. Impiego delle proteine di riserva per la identificazione delle cultivar di olivo (Olea europaea L.). In: Atti Qualità Olio Extravergine di Oliva, Firenze, 1–3 December, 1992, pp. 57–60.

    Google Scholar 

  • FAO (1991). Production Yearbook Series N. 76, Rome.

    Google Scholar 

  • Fiorino, F and A.R. Leva, 1986. Investigation on the micropropagation of the olive (Olea europaea L.) Influence of some mineral elements on the proliferation and rooting of explants. Olea 17: 101–104.

    Google Scholar 

  • Fodale, A.S., Mulè, R. and A. Tucci, 1993. Callus initiation from in vitro culture of olive ovules. In: The 2th International Symposium on Olive Growing, Jerusalem, September 6–10, 1993, p. 25.

    Google Scholar 

  • Fontanazza, G., 1987. Presentiamo la cultivar “I-77”. Terra e Vita (Speciale Olio e Olivo) 46: 10–11.

    Google Scholar 

  • James, D. J. , 1987. Cell and tissue culture technology for the genetic manipulation of temperature fruit trees. Biotechnology and Genetic Engineering Review Newcastle 5: 33–79.

    Google Scholar 

  • Johnson, L.A.S., 1957. A review of the family Oleaceae. Contribution from the National Herbarium 2: 395–418.

    Google Scholar 

  • Knoblauch, E., 1895. Oleaceae die naturlichen Pflanzenfamilien. In: Engler A., Prantl R. (eds.) 4: 1–16.

    Google Scholar 

  • Lavee, S. and N. Avidan, 1982. Growth responses of tree callus to chlorogenic acid and related phenolic substances. In: A. Fujiwara (Ed.), Proceedings Plant Tissue Culture 1982, pp. 165–168. Maruzen, Tokyo.

    Google Scholar 

  • Lavee, S. and G. Messer, 1969. The effect of growth-regulating substances and light on olive callus growth in vitro. J. Exp. Bot. 20: 604–614.

    Article  CAS  Google Scholar 

  • Lavee, S. and N. Adiri, 1974. The effects of abscisic acid and gibelleric acid on the development of apple and olive callus. In: Plant Growth Substances, Part VI. Tissue Culture Proceedings 18th International Conference, Hirokawa, Tokyo, pp. 1141–1148.

    Google Scholar 

  • Lavee, S., A. Haskal and M. Wodner, 1986. Barnea: a new olive cultivar from first breeding generation. Olea 17: 95–99.

    Google Scholar 

  • Leva, A.R., R. Petruccelli and A. Benelli, 1993. Plant regeneration and somatic embryogenesis in Olea europaea L. 6th European Congress on Biotechnology, Firenze, June 13-17, 1993, p. 364 (Abstr.).

    Google Scholar 

  • Leva, A.R., R. Petruccelli, R. Goretti and M. Panicucci, 1992. Ruolo di alcuni microelementi e carboidrati nella proliferazione in vitro di cv. di olivo (Olea europaea L.) In: Atti Qualità Olio Extravergine di Oliva, Firenze, 1–3 December, 1992, p. 333.

    Google Scholar 

  • Loukas, M. and C.B. Krimbas, 1983. History of olive cultivars based on their genetic distances. J. Hort. Sci. 58: 121–127.

    Google Scholar 

  • Mazzolani, G. and M.M. Altamura Betti, 1976/77. Elementi per la revisione del genere Olea (Tourn) Linn. 1 -Nota introduttiva. Ann. di Bot. 35-36: 463–469.

    Google Scholar 

  • Mazzolani, G. and M.M. Altamura Betti, 1979. Elementi per la revisione del genere Olea (Tourn). Linn. III -Olea Crysophylla Lam: forme affini. Ann. di Bot. 28: 15–31.

    Google Scholar 

  • Mazzolani, G. and M.M. Altamura Betti, 1980. Elementi per la revisione del genere Olea (Tourn) Linn. IV. Ciclo di Olea capensis L. Ann. di Bot. 28: 149–172.

    Google Scholar 

  • Mencuccini, M. and M. Lucchetti, 1992. Coltura di protoplasti isolati da differenti tessuti di cultivar di olivo (Olea europaea L.) In: Giornate Scientifiche S.O.I., Ravello, 8–10 April, 1992, pp. 146–147.

    Google Scholar 

  • Mencuccini, M. and E. Rugini, 1993. In vitro shoot regeneration from olive (Olea europaea L.) cultivar tissues. Plant Cell Tiss. Org. Cult. 32: 283–288.

    Article  CAS  Google Scholar 

  • Mitrakos, K., A. Alexaki and P. Papadimitriou, 1992. Dependance of olive morphogenesis on callus origin and age. J. Plant Physiol. 139: 269–273.

    Article  Google Scholar 

  • Morettini, A., 1972. Olivicoltura: Ramo Editoriale degli Agricoltori, Roma.

    Google Scholar 

  • Mulè, R., A.S. Fodale and A. Tucci, 1993. Attemps to generate haploid plants from in vitro cultures of Olea europaea L. anthers. In: The 2th International Symposium on Olive Growing, Jerusalem, September 6–10, 1993, p. 24.

    Google Scholar 

  • Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant 15: 473–492.

    Article  CAS  Google Scholar 

  • Orinos, T. and K. Mitrakos, 1991. Rhizogenesis and somatic embryogenesis in calli from wild olive [Olea europaea var. sylvestris (Miller) Lehr] mature zygotic embryos. Plant Cell Tiss. Org. Cult. 27: 183–187.

    Article  Google Scholar 

  • Pannelli, G., F. Famiani and E. Rugini, 1992a. Effects of the change on ploidy levels on anatomical, cytological, reproductive, growth performance and polyamine contents, in mutants of olive irradiated olive plants. Acta Hortic. 317: 209–218.

    Google Scholar 

  • Pannelli, G., R. Sirianni and M. Servili, 1992b. Preliminari osservazioni su una popolazione di accessioni di olivo selezionata in Umbria. In: Atti Germoplasma Frutticolo Alghero, 21-25 September, 1992, pp. 273–276.

    Google Scholar 

  • Parlati, M.V., E. Bellini and C. Menna, 1986. Selezione clonale dell’olivo nel Lazio. Primi risultati ottenuti nel Viterbese sulla cv. Canino Informatore Agrario 13: 25–29.

    Google Scholar 

  • Perri, E., M.V. Parlati and E. Rugini, 1993. Isolation and culture of olive (Olea europaea L.) In: The 2th International Symposium on Olive Growing, Jerusalem, September 6–10, 1993, p. 27.

    Google Scholar 

  • Perri, E., M.V. Parlati, A. Palapoli and A. Napolitano, 1992. Polimorfismo isoenzimatico in olivo. In: Giornate Scientifiche S.O.I., Ravello, 8–10 April, 1992, pp. 152–153.

    Google Scholar 

  • Pontikis, C.A., M. Loukas and G. Kousounis, 1980. The use of biochemical markers to distinguish olive cultivars. J. Hort. Sci. 55: 333–343.

    CAS  Google Scholar 

  • Prevost, G., G. Bartolini and C. Messeri, 1993. Cultivar Italiane di Olivo e Loro Sinonimi. Menegazzo, Lucca.

    Google Scholar 

  • Roselli, G. and B. Donini, 1982. “Briscola” Nuova cultivar di olivo a sviluppo compatto. Rivista Ortofrutticola Italiana 66: 103–114.

    Google Scholar 

  • Roselli, G., P. Rossi and G.G. Vendramin, 1992. Differenze isoenzimatiche in cultivar di olivo (Olea europea L.). Atti Congresso su Germoplasma Frutticolo, Alghero, 21–25 September, 1992, 113–118.

    Google Scholar 

  • Roselli, G., 1977. Osservazioni sulla scultura della exina del polline di alcune specie da frutto, 1. Olivo. Riv. Ortoflorofrutt. It. 3: 157–163.

    Google Scholar 

  • Roselli, G., 1990. Miglioramento genetico dell’olivo:la selezione del materiale di moltiplicazione e la tutela del germoplasma. Convegno “Nuove prospettive del vivaismo olivicolo”. Floricoltura Pesciatina 10: 2–6.

    Google Scholar 

  • Rugini, E. and S. Lavee, 1992. Olive Biotechnology. In: F.A. Hammerschlag and R. Litz (Eds.), Biotechnology of Perennial Fruit Crops, pp. 371–382. C.A.B. International Wallingford, Oxon U.K.

    Google Scholar 

  • Rugini, E., A. Pellegrineschi, M. Mencuccini and D. Mariotti, 1991b. Increase of rooting ability in the woody species Kiwi (Actinidia deliciosa A. Chev.) by transformation with Agrobacterium rhizogenes T-DNA rol genes. Plant Cell Rep. 10: 291–295.

    Article  CAS  Google Scholar 

  • Rugini, E. and E. Fedeli, 1990. Olive (Olea europaea L.) as an oilseed crop. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, Vol 10, pp. 593–641. Springer-Verlag, Berlin.

    Google Scholar 

  • Rugini, E. and G. Pannelli, 1992. Preliminary results on increasing fruit set in olive (Olea europaea L.) by chemical and mechanical treatments. Acta Hortic. 329: 209–210.

    Google Scholar 

  • Rugini, E. and P. Tarini, 1986. Somatic embryogenesis in olive (Olea europaea L.). Conference Fruit Tree Biotechnology, October 14–15, 1986, Paris, p. 62.

    Google Scholar 

  • Rugini, E., A. Jacoboni and M. Luppino, 1992. Role of basal shoot darkening and exogenous putrescine treatments on in vitro rooting and on endogenous polyamines changes in difficult-to-root woody species. Sci. Hort. 53: 63–72.

    Article  Google Scholar 

  • Rugini, E., 1981. Propagazione in vitro di una cultivar di olivo (Olea europaea L.). Valutazione di varie citochinine ed auxine. Atti Congresso Fitofarmaci in Agricoltura, Firenze, pp. 171–180.

    Google Scholar 

  • Rugini, E., 1984. In vitro propagation of some olive (Olea europaea L.) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos. Sci. Hort. 24: 123–134.

    Article  CAS  Google Scholar 

  • Rugini, E., 1988. Somatic embryogenesis and plant regeneration in Olive (Olea europaea L.). Plant Cell Tiss. Org. Cult. 14: 207–214.

    Article  Google Scholar 

  • Rugini, E., 1992. Involvement of polyamines in auxin and Agrobacterium rhizogenes-induced rooting of fruit trees in vitro. J. Amer. Hort. Sci. 117: 532–536.

    CAS  Google Scholar 

  • Rugini, E., G. Pannelli, M. Ceccarelli, M. Muganu and X. Quin, 1993. Selezione in vitro ed in vivo di piante poliploidi da mutanti mixoploidi di olivo cv Leccino e Frantoio. In: XXXVII Convegno Annuale della Società di Genetica Agraria, Orvieto, 11-14 October, 1993, p.166 (Abstr.).

    Google Scholar 

  • Rugini, E., M. Luppino, M. De Agazio and S. Grego, 1991a. Endogenous polyamine and root morphogenesis variations under different treatments in cuttings and in vitro explants of olive. Acta Hort. 300: 225–232.

    Google Scholar 

  • Rugini, E. and G. Caricato, 1994. Embriogenes: somatica organogenesi e produzione di piante do tessuti maturi di cultivar di olivo (Olea europaea L.) In: Atti Convegno su Tecniche, Norme e Qualita in olivicoltura, December 15–17, 1993 Potenza, p. 632–644.

    Google Scholar 

  • Rugini, E. and G. Caricato, 1995. Somatic embryogenesis and plant recovery from mature tissues of olive cultivars (Olea europaea L.) “Canino” and “Moraiols”, Plant Cell Reports (in press).

    Google Scholar 

  • Rugini, E., 1986. Olive. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry Trees, pp. 253–267. Springer-Verlag, Berlin.

    Google Scholar 

  • Sax, K. and E.C. Abbe, 1932. Chromosome numbers and the anatomy of the secondary xylem in the Oleaceae. J. Arnold Arboretum 23: 37–48.

    Google Scholar 

  • Scaramuzzi, F. and G. Roselli, 1986. Olive genetic improvement. Olea 17: 1–17.

    Google Scholar 

  • Schenk, R.V. and A.C. Hildebrandt, 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204.

    Article  CAS  Google Scholar 

  • Taylor, H., 1945. Cyto-taxonomy and phylogeny of the Oleaceae. Brittonia 5: 337–367.

    Article  Google Scholar 

  • Wang, K.J., P.F. Zhang, D.X. Ni, X.Z. Zhu, W.Q. Yang and Z.H. Bao, 1981. Callus formation and organ regeneration in tissue culture of woody plants. Acta Bot. Sinica 23: 97–108.

    Google Scholar 

  • White, P.R., 1963. The Cultivation of Animal and Plant Cells. Ronald Press, New York.

    Google Scholar 

Download references

Authors
  1. Eddo Rugini
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Plant Production, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Weyerhaeuser Inc., Tacoma, Washington, USA

    Pramod K. Gupta

  3. Department of Forest Science, Texas A & M University, College Station, Texas, USA

    Ronald J. Newton

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Rugini, E. (1995). Somatic embryogenesis in olive (Olea europaea L.). In: Jain, S.M., Gupta, P.K., Newton, R.J. (eds) Somatic Embryogenesis in Woody Plants. Forestry Sciences, vol 44-46. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0491-3_11

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-0491-3_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4220-8

  • Online ISBN: 978-94-011-0491-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation