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Cell-Free Protein Synthesis Using E. coli Cell Extract for NMR Studies

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Isotope labeling in Biomolecular NMR

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 992))

Abstract

The use of cell-free protein production systems for producing isotope labeled proteins generates new opportunities to perform unprecedented NMR studies. As compared with conventional cellular expression systems, the scrambling and dilution of amino acids are highly suppressed in the cell-free reaction, allowing the production of proteins with a wide variety of residue and site-specific isotope labeling patterns. In this chapter, the procedure for cell-free protein synthesis for NMR studies, using an E. coli extract, is introduced.

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Author information

Authors and Affiliations

  1. Structural Biology Research Center, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan

    Mitsuhiro Takeda & Masatsune Kainosho

  2. Center for Priority Areas, Graduate School of Science and Technology, Tokyo Metropolitan University, 1-1 Minami-ohsawa, Hachioji, 192-0397, Japan

    Masatsune Kainosho

Authors
  1. Mitsuhiro Takeda
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  2. Masatsune Kainosho
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Corresponding author

Correspondence to Masatsune Kainosho .

Editor information

Editors and Affiliations

  1. , NMR Research Centre, Indian Institute of Science, Bangalore, India

    Hanudatta S. Atreya

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Takeda, M., Kainosho, M. (2012). Cell-Free Protein Synthesis Using E. coli Cell Extract for NMR Studies. In: Atreya, H. (eds) Isotope labeling in Biomolecular NMR. Advances in Experimental Medicine and Biology, vol 992. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4954-2_9

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