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Transient Expression Using Agroinfiltration and Its Applications in Molecular Farming

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Molecular Farming in Plants: Recent Advances and Future Prospects

Abstract

Transient expression via agroinfiltration and/or viral vectors has quickly emerged as the preferred expression system for plant-made recombinant proteins. Transient expression can serve as a valuable research tool for finding optimal expression parameters before tedious and time-consuming production of stable transgenic plants or it can be scaled up to commercial production scale with vacuum infiltration. This technology is poised to compete with conventional production systems, large-scale production facilities are currently available and others are in the process of development. This chapter will introduce background and rationale in development of transient expression systems in plants and summarize the latest developments and examples in this area.

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Acknowledgement

This work was supported by a grant from an A-base grant from Agriculture and Agri-Food Canada and the Agricultural Bioproducts Innovation Program.

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Authors and Affiliations

  1. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford St., London, ON, N5V 4T3, Canada

    Rima Menassa & Adil Ahmad

  2. VTT Biotechnology, Technical Research Centre of Finland, 02044 VTT, Espoo, Finland

    Jussi J. Joensuu

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  1. Rima Menassa
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  2. Adil Ahmad
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  3. Jussi J. Joensuu
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Correspondence to Rima Menassa .

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Editors and Affiliations

  1. Agriculture and Agri-Food Canada, Sandford Street 1391, London, N5V 4T3, Ontario, Canada

    Aiming Wang

  2. , Immunology and Transplantation, Lawson Health Research Institute/Plantig, South Street 375, London, N6A4G5, Ontario, Canada

    Shengwu Ma

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Menassa, R., Ahmad, A., Joensuu, J.J. (2012). Transient Expression Using Agroinfiltration and Its Applications in Molecular Farming. In: Wang, A., Ma, S. (eds) Molecular Farming in Plants: Recent Advances and Future Prospects. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-2217-0_9

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