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Red Microalgae: From Basic Know-How to Biotechnology

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Red Algae in the Genomic Age

Part of the book series: Cellular Origin, Life in Extreme Habitats and Astrobiology ((COLE,volume 13))

Abstract

The group of Arad has taken up the challenge of exploiting the potential of the sulfated polysaccharides of red microalgae for biotechnological applications. To this end, they are conducting an integrated investigation, which initially involved chemical, physiological, and biochemical studies of the polysaccharide and the development of large-scale production technologies. However, as the group became more and more familiar with the subject, it became clear that the sulfated polysaccharides that characterize the red microalgae comprise a unique and very complex group of molecules and that biotechnology development would be correspondingly complex.

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References

  • Adda, M., Merchuk, J.C. and Arad (Malis), S. (1986) Effect of nitrate on growth and production of cell-wall polysaccharide by the unicellular red alga Porphyridium. Biomass 10: 131–140.

    Article  CAS  Google Scholar 

  • Apt, K.E., Grossman, A.R. and Kroth-Pancic, P.G. (1996) Stable nuclear transformation of the diatom Phaeodactylum tricornutum. Mol. Gen. Genet. 252: 572–579.

    PubMed  CAS  Google Scholar 

  • Arad (Malis), S. (1988) Production of sulfated polysaccharides from red unicellular algae, In: T. Stadler, J. Mollion and M.C. Verduset (eds.) Algal Biotechnology. Elsevier Applied Science, London, pp. 65–87.

    Google Scholar 

  • Arad (Malis), S. (1999) Polysaccharides of red microalgae, In: Z. Cohen (ed.) Chemicals from Microalgae. Taylor & Francis, New York, pp. 282–287.

    Google Scholar 

  • Arad (Malis), S., Adda, M. and Cohen, E. (1985) The potential of production of sulfated polysaccharides from Porphyridium. Plant Soil 89: 117–127.

    Article  Google Scholar 

  • Arad (Malis), S., Dubinsky, O. and Simon, B. (1993a) A modified cell wall mutant of the red microalga Rhodella reticulata resistant to the herbicide 2,6-dichlorobenzonitrile. J. Phycol. 29: 309–313.

    Article  Google Scholar 

  • Arad (Malis), S., Friedman (Dahan), O. and Rotem, A. (1988) Effect of nitrogen on polysaccharide production in Porphyridium sp. Appl. Environ. Microbiol. 54: 2411–2414.

    Google Scholar 

  • Arad (Malis), S., Keristovsky, G., Simon, B., Barak Z. and Geresh S. (1993b) Biodegradation of the sulphated polysaccharide of Porphyridium by soil bacteria. Phytochemistry 32: 287–290.

    Article  Google Scholar 

  • Arad (Malis), S., Kolani, R., Simon-Berkovitch, B. and Sivan, A. (1994) Inhibition by DCB of cell-wall polysaccharide formation in the red microalga Porphyridium sp. (Rhodophyta). Phycologia 33: 158–162.

    Article  Google Scholar 

  • Arad (Malis), S., Lerental (Brown), Y. and Dubinsky, O. (1992) Effect of nitrate and sulfate starvation on polysaccharide formation in Rhodella reticulata. Bioresour. Technol. 42: 141–148.

    Article  Google Scholar 

  • Arad (Malis), S., Rapoport, L., Moshkovich, A., van-Moppes, D., Karpasas, M., Golan, R. and Golan, Y. (2006) Superior biolubricant from a species of red microalga. Langmuir 22: 7313–7317.

    Article  CAS  Google Scholar 

  • Arad (Malis), S. and Richmond, A. (2004) Industrial production of microalgal cell-mass and secondary products – species of high potential: Porphyridium sp., In: A. Richmond (ed.) Handbook of Microalgal Culture: Biotechnology and Applied Phycology. Blackwell, UK, pp. 289–297.

    Google Scholar 

  • Armbrust, E.V. et al. (2004) The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. Science 306: 79–86.

    Article  PubMed  CAS  Google Scholar 

  • Asamizu, E., Nakajima, M., Kitade, Y., Saga, N., Nakamura, Y. and Tabata, S. (2003) Comparison of RNA expression profiles between the two generations of Porphyra yezoensis (Rhodophyta), based on expressed sequence tag frequency analysis. J. Phycol. 39: 923–930.

    Article  Google Scholar 

  • Barbier, G., Oesterhelt, C., Larson, M.D., Halgren, R.G., Wilkerson, C., Garavito, R.M., Benning, C. and Weber, A.P. (2005) Comparative genomics of two closely related unicellular thermo-acidophilic red algae, Galdieria sulphuraria and Cyanidioschyzon merolae, reveals the molecular basis of the metabolic flexibility of Galdieria sulphuraria and significant differences in carbohydrate metabolism of both algae. Plant Physiol. 137: 460–474.

    Article  PubMed  CAS  Google Scholar 

  • Burd, C.G., Strochlic, T.I. and Gangi Setty, S.R. (2004) Arf-like GTPases: not so Arf-like after all. Trends Cell Biol. 14: 687–694.

    Article  PubMed  CAS  Google Scholar 

  • Burger, G., Saint-Louis, D., Gray, M.W. and Lang, B.F. (1999) Complete sequence of the mitochondrial DNA of the red alga Porphyra purpurea: cyanobacterial introns and shared ancestry of red and green algae. Plant Cell 11: 1675–1694.

    PubMed  CAS  Google Scholar 

  • Christie, J.M. (2007) Phototropin blue-light receptors. Annu. Rev. Plant Biol. 58: 21–45.

    Article  PubMed  CAS  Google Scholar 

  • Cohen, E. and Arad (Malis), S. (1989) A closed system for outdoor cultivation of Porphyridium. Biomass 18: 59–67.

    Article  Google Scholar 

  • Dubinsky, O., Simon, B., Karamanos, Y., Geresh, S., Barak, Z. and Arad (Malis), S. (1992) Composition of the cell-wall polysaccharide produced by the unicellular red alga Rhodella reticulata. Plant Physiol. Biochem. 30: 409–414.

    CAS  Google Scholar 

  • Dvir, I., Chayoth, R., Sod-Moriah, U., Shany, S., Nyska, A., Stark, A.H., Madar, Z. and Arad (Malis), S. (2000) Soluble polysaccharide and biomass of red microalga Porphyridium sp. alter intestinal morphology and reduce serum cholesterol in rats. Br. J. Nutr. 84: 469–476.

    PubMed  CAS  Google Scholar 

  • Dvir, I., Geva, O., Chayoth, R., Sod-Moriah, U., Shany, S., Arad, S. and Madar, Z. (1996). Polysaccharides and algal biomass as new sources of dietary fibers and their physiological effects in rats. FASEB J. 10: 3012–3012.

    Google Scholar 

  • Eteshola, E., Gottlieb, M. and Arad (Malis), S. (1996) Dilute solution viscosity of red microalga exopolysaccharide. Chem. Eng. Sci. 51: 1487–1494.

    Article  CAS  Google Scholar 

  • Eteshola, E., Karpasas, M., Arad (Malis), S. and Gottlieb, M. (1998) Red microalga exopolysaccharides: 2. Study of the rheology, morphology and thermal gelation of aqueous preparations. Acta Polym. 49: 549–556.

    Article  CAS  Google Scholar 

  • Falciatore, A., Casotti, R., Leblanc, C., Abrescia, C. and Bowler, C. (1999) Transformation of nonselectable reporter genes in marine diatoms. Marine Biotech. 1: 239–251.

    Article  CAS  Google Scholar 

  • Fletcher, S.P., Muto, M. and Mayfield, S. (2007) Optimization of recombinant protein expression in the chloroplasts of green algae, In: R. Leon, A. Gavan and E. Fernandez (eds.) Transgenic Microalgae as Green Cell Factories, Vol. 616. Springer, Dordrecht, pp. 90–98.

    Chapter  Google Scholar 

  • Frey, A.D. and Kallio, P.T. (2003) Bacterial hemoglobins and flavohemoglobins: versatile proteins and their impact on microbiology and biotechnology. FEMS Microbiol. Rev. 27: 525–545.

    Article  PubMed  CAS  Google Scholar 

  • Friedman, O., Dubinsky, Z. and Arad (Malis), S. (1991) Effect of light intensity on growth and polysaccharide production in red and blue-green Rhodophyta unicells. Bioresourc. Technol. 38: 105–110.

    Article  CAS  Google Scholar 

  • Galvan, A., Gozalez-Ballester, D. and Fernandez, E. (2007) Insertional mutagenesis as tool to study genes/functions in Chlamydomonas, In: R. Leon, A. Galvan and E. Fernandez (eds.) Transgenic Microalgae as Green Cell Factories, Vol. 616. Springer, Dordrecht, pp. 77–89.

    Chapter  Google Scholar 

  • Gao, Y., Schofield, O. and Leustek, T. (2000) Characterization of sulfate assimilation in marine algae focusing on the enzyme 5′-adenylylsulfate reductase. Plant Physiol. 123(3): 1087–1096.

    Article  PubMed  CAS  Google Scholar 

  • Geresh, S. and Arad (Malis), S. (1991). The extracellular polysaccharides of the red microalgae: chemisrtry and rheology. Bioresourc. Technol. 38: 195–201.

    Article  CAS  Google Scholar 

  • Geresh, S., Dubinsky, O., Arad (Malis), S., Christiaen, D. and Glaser, R. (1990) Structure of 3-O-(alpha-D-glucopyranosyluronic acid)-L-galactopyranose, an aldobiouronic acid isolated from the polysaccharides of various unicellular red algae. Carbohydr. Res. 208: 301–305.

    Article  PubMed  CAS  Google Scholar 

  • Geresh, S., Lupescu, N. and Arad (Malis), S. (1992) Fractionation and partial characterization of the sulfated polysaccharide of the red microalga Porphyridium sp. Phytochemistry 31(12): 4181–4186.

    Article  CAS  Google Scholar 

  • Ginzberg, A., Cohen, M., Sod-Moriah, U.A., Shany, S., Rosenshtrauch, A. and Arad, S.M. (2000) Chickens fed with biomass of the red microalga Porphyridium sp. have reduced blood cholesterol level and modified fatty acid composition in egg yolk. J. Appl. Phycol. 12: 325–330.

    Article  Google Scholar 

  • Glockner, G., Rosenthal, A. and Valentin, K. (2000) The structure and gene repertoire of an ancient red algal plastid genome. J. Molec. Evolut. 51: 382–390.

    CAS  Google Scholar 

  • Gourdon, D., Lin, Q., Oroudjev, E., Hansma, H., Golan, Y., Arad, S. and Israelachvili, J. (2008) Adhesion and stable low friction provided by a subnanometer-thick monolayer of a natural polysaccharide. Langmuir (in press).

    Google Scholar 

  • Griesbeck, C., Kobl, I. and Heitzer, M. (2006) Chlamydomonas reinhardtii: a protein expression system for pharmaceutical and biotechnological proteins. Mol. Biotechnol. 34: 213–223.

    Article  PubMed  CAS  Google Scholar 

  • Grossman, A.R. (2005) Paths toward algal genomics. Plant Physiol. 137: 410–427.

    Article  PubMed  CAS  Google Scholar 

  • Grossman, A.R. (2007) In the grip of algal genomics, In: R. León, A. Galván, and E. Fernández (eds.) Transgenic Microalgae as Green Cell Factories, Advances in Experimental Medicine and Biology, Vol. 616. Springer, Dordrecht, 132 pp.

    Google Scholar 

  • Gruber, A., Vugrinec, S., Hempel, F., Gould, S., Maier, U.-G. and Kroth, P. (2007) Protein targeting into complex diatom plastids: functional characterisation of a specific targeting motif. Plant Molec. Biol. 64: 519–530.

    Article  CAS  Google Scholar 

  • Hallman, A. (2007) Algal transgenic and biotechnology. Transgenic Plant J. 1(1): 89–98.

    Google Scholar 

  • Hawkins, R.L. and Nakamura, M. (1999) Expression of human growth hormone by the eukaryotic Alga, Chlorella. Curr. Microbiol. 38: 335–341.

    Article  CAS  Google Scholar 

  • Heaney-Kieras, J. and Chapman, D.J. (1976) Structural studies on the extracellular polysaccharide of the red alga Porhyridium cruentum. Carbohydr. Res. 52: 169–177.

    Article  PubMed  CAS  Google Scholar 

  • Heaney-Kieras, J., Roden, L. and Chapman, D.J. (1977) The covalent linkage of protein to carbohydrate in the extracellular protein-polysaccharide from the red alga Porphyridium cruentum. Biochem. J. 165: 1–9.

    PubMed  CAS  Google Scholar 

  • Huang, X. and Madan, A. (1999) CAP3: a DNA sequence assembly program. Genome Res. 9: 868–877.

    Article  PubMed  CAS  Google Scholar 

  • Huleihel, M., Ishanu, V., Tal, J. and Arad (Malis), S. (2002) Activity of Porphyridium sp ­polysaccharide against herpes simplex viruses in vitro and in vivo. J. Biochem. Biophys. Meth. 50: 189–200.

    Article  Google Scholar 

  • Huleihel, M., Ishanu, V., Tal, J. and Arad, S. (2001) Antiviral effect of red microalgal polysaccharides on Herpes simplex and Varicella zoster viruses. J. Appl. Phycol. 13: 127–134.

    Article  CAS  Google Scholar 

  • Jarvis, E.E. and Brown, L.M. (1991) Transient expression of firefly luciferase in protoplasts of the green alga Chlorella ellipsoidea. Curr. Genet. 19: 317–321.

    Article  CAS  Google Scholar 

  • Jaseja, M., Perlin, A.S., Dubinsky, O., Christiaen, D., (Malis) Arad, S. and Glaser, R. (1989) NMR structure determination of 3-O-(α-D-glucopyranosyluronic acid)-L-galactopyranose, an aldobiuronic acid isolated from the unicellular red alga Rhodella reticulata. Carbohydr. Res. 186: 313–319.

    Article  CAS  Google Scholar 

  • Keidan, M., Broshy, H., van Moppes, D. and Arad (Malis), S. (2006) Assimilation of sulphur into the cell-wall polysaccharide of the red microalga Porphyridium sp. (Rhodophyta). Phycologia 45: 505–511.

    Article  Google Scholar 

  • Keidan, M., Friedlander, M. and Arad (Malis), S. (2008) Effect of Brefeldin A on cell-wall polysaccharide production in the red microalga Porphyridium sp. (Rhodophyta) through its effect on the Golgi apparatus. Phycologia (in press).

    Google Scholar 

  • Lapidot, M., Raveh, D., Sivan, A., Arad (Malis), S. and Shapira, M. (1999) Molecular analysis of the AHAS gene of Porphyridium sp. (Rhodophyta) and of a mutant resistant to sulfometuron methyl. J. Phycol. 35: 1233–1236.

    Article  CAS  Google Scholar 

  • Lapidot, M., Raveh, D., Sivan, A., Arad, S.M. and Shapira, M. (2002) Stable chloroplast transformation of the unicellular red alga Porphyridium sp. Plant Physiol. 129: 7–12.

    Article  PubMed  CAS  Google Scholar 

  • Li, S.-Y., Lellouche, J.-P., Shabtai, Y. and Arad, S. (2001) Fixed carbon partitioning in the red microalga Porphyridium sp. (Rhodophyta). J. Phycol. 37: 289–297.

    Article  CAS  Google Scholar 

  • Li, S.Y., Shabtai, Y. and Arad (Malis), S. (2000) Production and composition of the sulphated cell-wall polysaccharide of Porphyridium sp. (Rhodophyta) as affected by CO2 concentration. Phycologia 39: 332–336.

    Article  Google Scholar 

  • Lisacek, F.C., Traini, M.D., Sexton, D., Harry, J.L. and Wilkins, M.R. (2001) Strategy for protein isoform identification from expressed sequence tags and its application to peptide mass fingerprinting. Proteomics 1: 186–193.

    Article  PubMed  CAS  Google Scholar 

  • Lluisma, A. and Ragan, M. (1997) Expressed sequence tags (ESTs) from the marine red alga Gracilaria gracilis. J. Appl. Phycol. 9: 287–293.

    Article  CAS  Google Scholar 

  • Lupescu, N., Arad (Malis), S., Geresh, S., Bernstein, M. and Glazer, R. (1991) Structure of some sulfated sugars isolated after acid hydrolysis of the extracellular polysaccharide of Porphyridium sp. a unicellular red alga. Carbohydr. Res. 210: 349–352.

    Article  CAS  Google Scholar 

  • Lupescu, N., Solo-Kwan, J., Christiaen, D., Morvan, H. and Arad (Malis), S. (1992). Structural determination by means of gas-chromatography-mass spectrometry of 3-O-(α-D-glucopyranosyluronic acid)-galactopyranose, an aldobiuronic acid derived from Porphyridium sp. polysaccharide. Carbohydr. Polym. 2: 131–134.

    Article  Google Scholar 

  • Manandhar-Shrestha, K. (2007) Immunological aspects of the red microalga Porphyridium sp. based vaccine. Ph.D thesis, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

    Google Scholar 

  • Marchler-Bauer, A. et al. (2003) CDD: a curated Entrez database of conserved domain alignments. Nucleic Acids Res. 31: 383–387.

    Article  PubMed  CAS  Google Scholar 

  • Matsuzaki, M. et al. (2004) Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature 428: 653–657.

    Article  PubMed  CAS  Google Scholar 

  • Matsui, M.S., Muizzuddin, N., Arad, S. and Marenus, K. (2003) Sulfated polysaccharides from red microalgae have antiinflammatory properties in vitro and in vivo. Appl. Biochem. Biotechnol. 104: 13–22.

    Article  PubMed  CAS  Google Scholar 

  • Mayfield, S.P., Franklin, S.E. and Lerner, R.A. (2003) Expression and assembly of a fully active antibody in algae. PNAS 100: 438–442.

    Article  PubMed  CAS  Google Scholar 

  • Merchant, S.S. et al. (2007) The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science 318: 245–250.

    Article  PubMed  CAS  Google Scholar 

  • Merchuk, J.C., Ronen, M., Giris, S. and Arad (Malis), S. (1998) Light/dark cycles in the growth of the red microalga Porphyridium sp. Biotechnol. Bioengin. 59(6): 705–713.

    Article  CAS  Google Scholar 

  • Minoda, A., Sakagami, R., Yagisawa, F., Kuroiwa, T. and Tanaka, K. (2004) Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, Cyanidioschyzon merolae 10D. Plant Cell Physiol. 45: 667–671.

    Article  PubMed  CAS  Google Scholar 

  • Moody, D.E. (2001) Genomics techniques: an overview of methods for the study of gene expression. J. Anim. Sci. 79: E128–135.

    Google Scholar 

  • Moreira, D., Le Guyader, H. and Philippe, H. (2000) The origin of red algae and the evolution of chloroplasts. Nature 405: 32–33.

    Article  Google Scholar 

  • Mukai, M., Mills, C.E., Poole, R.K. and Yeh, S.-R. (2001) Flavohemoglobin, a globin with a peroxidase-like catalytic site. J. Biol. Chem. 276: 7272–7277.

    Article  PubMed  CAS  Google Scholar 

  • Nikaido, I., Asamizu, E., Nakajima, M., Nakamura, Y., Saga, N. and Tabata, S. (2000) Generation of 10,154 expressed sequence tags from a leafy gametophyte of a marine red alga, Porphyra yezoensis. DNA Res. 7: 223–227.

    Article  PubMed  Google Scholar 

  • Noguchi, T., Watanabe, H. and Suzuki, R. (1998) Effects of brefeldin A on the Golgi apparatus, the nuclear envelope, and the endoplasmic reticulum in a green alga, Scenedesmus acutus. Protoplasma 201: 202–212.

    Article  CAS  Google Scholar 

  • Nozaki, H. et al. (2007) A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae. BMC Biol. 5: 28.

    Article  PubMed  CAS  Google Scholar 

  • Oesterhelt, C., Vogelbein, S., Shrestha, R., Stanke, M. and Weber, A. (2008) The genome of the thermoacidophilic red microalga Galdieria sulphuraria encodes a small family of secreted class III peroxidases that might be involved in cell wall modification. Planta 227: 353–362.

    Article  PubMed  CAS  Google Scholar 

  • Ohta, N., Matsuzaki, M., Misumi, O., Miyagishima, S.-Y., Nozaki, H., Tanaka, K., Shin-I, T., Kohara, Y. and Kuroiwa, T. (2003) Complete sequence and analysis of the plastid genome of the unicellular red alga Cyanidioschyzon merolae. DNA Res. 10: 67–77.

    Article  PubMed  CAS  Google Scholar 

  • Oliveira, M.C. and Bhattacharya, D. (2000) Phylogeny of the Bangiophycidae (Rhodophyta) and the secondary endosymbiotic origin of algal plastids. Am. J. Bot. 87: 482–492.

    Article  PubMed  CAS  Google Scholar 

  • Otero, A. and Vincenzini, M. (2004) Nostoc (Cyanophyceae) goes nude: extracellular polysaccharides serve as a sink for reducing power under unbalanced C/N metabolism. J. Phycol. 40: 74–81.

    Article  CAS  Google Scholar 

  • Plesser, L. (2004) Development of a nuclear transformation system for the red microalga Porphyridium sp. M.Sc thesis, Ben Gurion University of the Negev, Beer-Sheva, Israel.

    Google Scholar 

  • Plesser, L., Lapidot, M., Weinstein, Y. and Arad (Malis) S. (2007) Sulfotransferase of red microalgae: a molecular and biochemical study. 8th Marine Biotechnology Conference March 2007. Eilat, P-31.

    Google Scholar 

  • Pulz, O. and Gross, W. (2004) Valuable products from biotechnology of microalgae. Appl. Microbiol. Biotechnol. 65: 635–648.

    Article  PubMed  CAS  Google Scholar 

  • Purton, S. (2007) Tools and techniques for chloroplast transformation of Chlamydomonas, In: R. Leon, A. Gavan and E. Fernandez (eds.) Transgenic Microalgae as Green Cell Factories, Vol. 616. Springer, Dordrecht, pp. 34–45.

    Chapter  Google Scholar 

  • Quackenbush, J. et al. (2001) The TIGR Gene Indices: analysis of gene transcript sequences in highly sampled eukaryotic species. Nucleic Acids Res. 29: 159–164.

    Article  PubMed  CAS  Google Scholar 

  • Ragan, M. and Gutell, P. (1995) Are red algae plants? Bot. J. Linn. Soc. 118: 81–105.

    Google Scholar 

  • Ramus, J. (1972) The production of extracellular polysaccharides by the unicellular red algal Porphyridium aerugineum. J. Phycol. 8: 97–111.

    CAS  Google Scholar 

  • Ramus, J. and Groves, S.T. (1972) Incorporation of sulfate into the capsular polysaccharide of the red alga Porphyridium. J. Cell Biol. 54: 399–407.

    Article  PubMed  CAS  Google Scholar 

  • Ramus, J. and Groves, T. (1974) Precursor–product relationships during sulfate incorporation into Porphyridium capsular polysaccharide. Plant Physiol. 53: 434–439.

    Article  PubMed  CAS  Google Scholar 

  • Ramus, J. and Robins, D.M. (1975) The correlation of Golgi activity and polysaccharide secretion in Porphyridium. J Phycol 11: 70–74.

    CAS  Google Scholar 

  • Reith, M. and Munholland, J. (1995) Complete nucleotide sequence of the Porphyra purpurea chloroplast genome. Plant Mol. Biol. Repr. 13: 333–335.

    Article  CAS  Google Scholar 

  • Shrestha, R.P., Weinstein, Y., Bar-Zvi, D. and Arad, S.M. (2004) A glycoprotein noncovalently associated with cell-wall polysaccharide of the red microalga Porphyridium sp. (Rhodophyta). J. Phycol. 40: 568–580.

    Article  CAS  Google Scholar 

  • Simon-Bercovitch, B., Bar-Zvi, D. and Arad (Malis), S. (1999) Cell-wall formation during the cell cycle of Porphyridium sp. (Rhodophyta). J. Phycol. 35: 78–83.

    Article  Google Scholar 

  • Sivan, A. and Arad (Malis), S. (1993) Induction and characterization of pigment mutants in the red microalga Porphyridium sp (Rhodophyceae). Phycologia 32: 68–72.

    Article  Google Scholar 

  • Sivan, A. and Arad (Malis), S. (1995) A mutant of the red microalga Porphyridium sp. (Rhodophyceae) resistant to DCMU and atrazine. Phycologia 34: 299–305.

    Article  Google Scholar 

  • Sivan A., van Moppes, D. and Arad (Malis) S. (1995) Protoplast fusion and genetic complementation of pigment mutations in the red microalga Porphyridium sp. J. Phycol. 31: 167–172.

    Article  CAS  Google Scholar 

  • Sivan, A. and Arad (Malis), S. (1998) Intraspecific transfer of herbicide resistance in the red microalga Porphyridium sp. (Rhodophyceae) via protoplast fusion. J. Phycol. 34: 706–711.

    Article  Google Scholar 

  • Singh, S., Arad (Malis) S. and Richmond, A. (2000) Extracellular polysaccharide production in outdoor mass cultures of Porphyridium sp. in flat plate glass reactors. J. Appl. Phycol. 12: 269–275.

    Article  CAS  Google Scholar 

  • Tannin-Spitz, T., Bergman, M., van-Moppes, D., Grossman, S. and Arad (Malis), S. (2005) Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp. J. Appl. Phycol. 17: 215–222.

    Article  CAS  Google Scholar 

  • van Moppes, D., Barak, Z., Chipman, D.M., Gollop, N. and Arad (Malis), S. (1989) An herbicide (sulfometuron methyl) resistant mutant in Porphyridium (Rhodophyta). J. Phycol. 25: 108–112.

    Article  Google Scholar 

  • Weber, A. et al. (2004) EST-analysis of the thermo-acidophilic red microalga Galdieria sulphuraria reveals potential for lipid A biosynthesis and unveils the pathway of carbon export from rhodoplasts. Plant Mol. Biol. 55: 17–32.

    Article  PubMed  CAS  Google Scholar 

  • Yaron, A. and Arad (Malis), S. (1993) Natural pigments from red microalgae for food and cosmetics, In: G. Charalambous (ed.) Food Flavors, Ingredients and Composition. Elsevier Science Publishers B.V., Amsterdam, pp. 835–839.

    Google Scholar 

  • Yaron, A., Dvir, I., Maislos, M., Mokady, S. and Arad (Malis), S. (1995) The red microalga Rhodella reticulata as a source of a dietary ω-3 highly unsaturated fatty acid-ecosapentaenoic acid, In: Charalambous (ed.) Food Flavors: Generation, Analysis and Process Influence. Elsevier Science B.V., Amsterdam, pp. 665–674.

    Chapter  Google Scholar 

  • Yoon, H.S., Hackett, J.D., Pinto, G. and Bhattacharya, D. (2002) From the cover: the single, ancient origin of chromist plastids. Proc. Natl. Acad. Sci. USA. 99: 15507–15512.

    Article  PubMed  CAS  Google Scholar 

  • Yoon, H.S., Muller, K.M., Sheath, R.G., Ott, F.D. and Bhattacharya, D. (2006) Defining the major lineages of red algae (Rhodophyta). J. Phycol. 42: 482–492.

    Article  CAS  Google Scholar 

  • Zaslavskaia, L.A., Lippmeier, J.C., Kroth, P.G., Grossman, A.R. and Apt, K.E. (2000) Transformation of the diatom Phaeodactylum tricornutum (Bacillariophyceae) with a variety of selectable marker and reporter genes. J. Phycol. 36: 379–386.

    Article  CAS  Google Scholar 

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Author notes

  1. Shoshana Arad (Malis)

    Present address: Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, 92037, USA

Authors and Affiliations

  1. Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel

    Miri Lapidot, Roshan Prakash Shrestha & Shoshana Arad (Malis)

  2. Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel

    Yacob Weinstein

Authors
  1. Miri Lapidot
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  2. Roshan Prakash Shrestha
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  3. Yacob Weinstein
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  4. Shoshana Arad (Malis)
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Corresponding author

Correspondence to Shoshana Arad (Malis) .

Editor information

Editors and Affiliations

  1. University of Jerusalem, Mevo Hadas 20, Efrat, 90435, Israel

    Joseph Seckbach

  2. Marine Science Institute, University of California, Santa Barbara, Santa Barbara, 93106, USA

    David J. Chapman

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Lapidot, M., Shrestha, R.P., Weinstein, Y., Arad (Malis), S. (2010). Red Microalgae: From Basic Know-How to Biotechnology. In: Seckbach, J., Chapman, D. (eds) Red Algae in the Genomic Age. Cellular Origin, Life in Extreme Habitats and Astrobiology, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3795-4_11

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