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Actin-Induced Elongation of Fibers Composed of Cytoplasmic Membrane from Nitella

  • Chapter
Cell Dynamics

Part of the book series: Protoplasma ((PROTOPLASMA,volume 2))

Summary

As reported previously, cytoplasmic membrane originated from endoplasmic droplets of Nitella formed networks of fibrils spread on a glass slide in the activating medium containing Mg-ATP and sucrose, and these fibrils were referred to as the network fibers [Higashi-Fujime S (1980) J Cell Biol 87: 569–578].

After spreading on the glass slide, this network fiber was induced to elongate by muscle actin in ATP dependent manner. Projections were newly formed in the middle of the network fibers and elongated in a straight line on the glass surface. The direction of elongation changed only when it reached on obstacle such as other network fibers or granules, and formed a kink. Elongating fibers suddenly stopped, often retracted and absorbed into the network fiber again. Sometimes they were broken or made bridges between stationary network fibers. Due to these movements, the network pattern was continuously changing. Projections reached about 100 µm long maximally and the maximum speed of elongation was about 30 µm/s. The movement continued for about 5 min after addition of actin. Electron micrographs showed membraneous structures in the fiber.

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Abbreviations

ATP:

adenosine 5′-triphosphate

EGTA:

[ethylenebis(oxyethylenenitrilo)]tetraacetic acid

NEM:

N-ethylmaleimide

DTT:

dithiothreitol

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Author information

Authors and Affiliations

  1. Department of Molecular Biology, Faculty of Science, Nagoya University Chikusa-ku, Nagoya, Japan

    S. Higashi-Fujime

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  1. S. Higashi-Fujime
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Editor information

Editors and Affiliations

  1. Department of Botany, Faculty of Science, University of Tokyo, Tokyo, Japan

    Masashi Tazawa Ph.D.

Additional information

Dediacted to Professor Dr. Noburo Kamiya on the occasion of his 75th birthday

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© 1988 Springer-Verlag

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Higashi-Fujime, S. (1988). Actin-Induced Elongation of Fibers Composed of Cytoplasmic Membrane from Nitella . In: Tazawa, M. (eds) Cell Dynamics. Protoplasma, vol 2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9011-1_4

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  • DOI: https://doi.org/10.1007/978-3-7091-9011-1_4

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-9013-5

  • Online ISBN: 978-3-7091-9011-1

  • eBook Packages: Springer Book Archive

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