Summary
Latex beads coated with rabbit skeletal muscle myosin were introduced by intracellular perfusion intoChara cells from which the tonoplasts had been removed. Mg · ATP dependent movement of the beads along files ofChara chloroplast layers was observed. The movement was in opposite directions on the two sides of the indifferent line, indicating that the movement was dependent on the polarity of the actin bundles. This suggests that the unknown factor responsible for generating the motive force for cytoplasmic streaming inChara endoplasm is myosin. The advantages of the present experimental system for studying the sliding mechanism of actomyosin are discussed.
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Abbreviations
- APW:
-
artificial pond water
- ATP:
-
adenosine 5′-triphosphoric acid
- DTT:
-
dithiothreitol
- EDTA:
-
ethylenediamine tetraacetic acid
- EGTA:
-
ethyleneglycol-bis(β-aminoethyl ether)N, N, N′, N′-tetraacetic acid
- HMM:
-
heavy meromyosin
- LMM:
-
light meromyosin
- NEM:
-
N-ethylmaleimide
- PIPES:
-
piperazine-N, N′- bis(2-ethanesulfonic acid)
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Shimmen, T., Yano, M. Active sliding movement of latex beads coated with skeletal muscle myosin onChara actin bundles. Protoplasma 121, 132–137 (1984). https://doi.org/10.1007/BF01279760
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DOI: https://doi.org/10.1007/BF01279760