Abstract
Nuclei of the cornea and of several cell types occurring in the retina (pigmented epithelium, bipolar cells, rods and cones, and Müller cells) were studied with respect to their DNA content. chromatin ultrastructure, and RNA synthesizing activity. Significant differences were found. It is concluded that transcription activity depends on the proportion of decondensed chromatin, and that both may be controlled by differential DNA replication during early development of the eye.
A preliminary report.
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References
Allfrey, V. G., Littau, V. C., Mirsky, A. E., 1963: On the role of histones in regulating RNA synthesis in the cell nucleus. — Proc. Nat. Acad. Sci. (U.S.) 49, 414–421.
Auer, G., Zetterberg, A., 1972: The role of nuclear proteins in RNA synthesis. — Exp. Cell Res. 75, 245–253.
Britten, R. J., Davidson, R. H., 1969: Gene regulation for higher cells: a theory. — Science 165, 349–357.
Britten, R. J., Davidson, R. H., 1971: Repetitive and non-repetitive DNA sequences and a speculation on the origins of evolution novelty. — Quart. Rev. Biol. 46, 111–138.
Bulow, N., 1975: The retinal pigment epithelium and photoreceptor cells on monkey eyes. — Cell Tiss. Res. 161, 521–540.
Busch, H., (Ed.), 1978: The Cell Nucleus, vol. 4 5: Chromatin. — New York: Academic Press. Cold Spring Harbor Symposia Quant. Biol. 42: Chromatin (1978).
Hsu, T. C., 1962: Differential rate in RNA synthesis between euchromatin and heterochromatin. — Exp. Cell Res. 27, 332–334.
Johns, E. W., 1971: Histones, chromatin structure and RNA synthesis. — Nature New Biol. 237, 87–88.
Kaneko, A., Lam, D. M. K., Wiesel, T. N., 1976: Isolated horizontal cells of elasmobranch retinae. — Brain Res. 105, 567–572.
Kuenzle, C. C., Bregnard, A., Hübscher, U., Ruc H, F., 1978: Extra DNA in forebrain cortical neurons. — Exp. Cell Res. 113, 151–160.
Magalhaes, M. M., Coimbra, A., 1972: The rabbit retina Müller cell. A fine structural and cytochemical study. — J. Ultrastruct. Res. 39, 310–326.
Mccarthy, B. J., Nishiura, J. T., Doenecke, D., Nasser, D. S., Johnson, C. B., 1974: Transcription and chromatin structure. — Cold Spring Harbor Symp. Quant. Biol. 38, 763–771.
Mirsky, A. E., Silverman, B., 1973: Effect of selective extraction of histones on template activities of human chromatin by use of exogenous DNA and RNA polymerases. — Proc. Nat. Acad. Sci. (U.S.) 70, 1973–1975.
Nagl, W., 1969: Correlation of structure and RNA synthesis in the nucleolus-organizing polytene chromosomes of Phaseolus vulgaris. — Chromosoma 28, 85–91.
Nagl, W., 1976: Nuclear organization. — Ann. Rev. Pl. Physiol. 27, 39–69.
Nagl, W., 1977: The DNA optimization model for speciation and cytodifferentiation. — Chrom. Today 6, 151–152.
Nagl, W., 1978: Endopolyploidy and Polyteny in Differentiation and Evolution. — Amsterdam: North-Holland.
Nagl, W., 1979: Condensed interphase chromatin in plant and animal cell nuclei: fundamental differences. — Pl. Syst. Evol., Suppl. 2, 247–260.
Nagl, W., Frisch, B., Frülich, E., 1979: Extra DNA synthesis involved in floral differentiation. — Pl. Syst. Evol., Suppl. 2, 211–218.
Nguyen-Legros, J., 1978: Fine structure of the prigment epithelium in the vertebrate retina. — Intern. Rev. Cytol., Suppl. 7, 464–499.
Rasmussen, K. E., 1973: A morphometric study of the Müller cells, their nuclei and mitochondria in the rat retina. — J. Ultrastruct. Res. 44, 96–112.
Paul, J., Gilmour, R. S., 1968: Organ-specific restiction of transcription in mammalian chromatin. — J. Molec. Biol. 34, 305–316.
Schaffner, K.-H., Nagl, W., 1979: Differential DNA replication involved in transition from juvenile to adult phase in Hedera helix. — Pl. Syst. Evol., Suppl. 2, 105–110.
Smart, J. E., Bonner, J., 1971: Study on the role of histones in relation to the template activity and precipitability of chromatin at physiological ionic strengths. — J. Molec. Biol. 58, 675–684.
Strom, C. M., Moscona, M., Dorfman, A., 1978: Amplification of DNA sequences during cartilage and neural retina differentiation. — Proc. Nat. Acad. Sci. (U.S.) 75, 4451–4454.
Thomas, C., Schram, A., 1977: Correlation between the condensation state of rDNA and rRNA synthesis during Xenopus laevis oogenesis. — Biol. Cell, 30, 49–54.
Zuckerkandl, E., 1976: Gene control in eukaryotes and the C-value paradox. “Excess” DNA as an impedient to transcription of coding sequences. — J. Mol. Evol. 9, 73–104.
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Schmalenberger, B., Nagl, W. (1979). Different DNA Content, Chromatin Condensation, and Transcription Activity in Retina Cell Nuclei of the Guinea-Pig. In: Nagl, W., Hemleben, V., Ehrendorfer, F. (eds) Genome and Chromatin: Organization, Evolution, Function. Plant Systematics and Evolution, vol 2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-8556-8_10
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DOI: https://doi.org/10.1007/978-3-7091-8556-8_10
Publisher Name: Springer, Vienna
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