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Correlation of structure and RNA synthesis in the nucleolus-organizing polytene chromosomes of Phaseolus vulgaris

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Abstract

The relationship between RNA synthesis and morphology of the nucleolus-organizing polytene chromosomes in the highly endopolyploid suspensor cells of Phaseolus vulgaris has been studied by actinomycin D treatment, temperature lowering, and H3-uridine autoradiography. Actinomycin D and low temperature induce a condensation of the giant chromosomes, particularly of the nuoleolus organizers and of the intranucleolar regions of the chromosomes. RNA synthesis occurs in the extended state of the chromosomes, but it ceases in the highly condensed state caused by the treatment of the cells either with actinomycin D or with low temperature.

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References

  • Barr, H. J., and W. Plant: Comparative morphology of nucleolar DNA in Drosophila. J. Cell. Biol. 31, C17-C22 (1966).

    Google Scholar 

  • Beermann, W.: Control of differentiation at the chromosomal level. J. exp. Zool. 157, 49–62 (1964).

    Google Scholar 

  • —: Gene action at the level of chromosomes. In: Cell differentiation and morphogenesis, p. 24–54. Amsterdam: North Holland Publ. Co. 1966.

    Google Scholar 

  • Berendes, H. D.: Factors involved in the expression of gene activity in polytene chromosomes. Chromosoma (Berl.) 24, 418–437 (1968).

    Google Scholar 

  • Clever, U.: Control of chromosome puffing. In: The control of nuclear activity, p. 161–186. Englewood Cliffs: Prentice Hall Inc. 1967.

    Google Scholar 

  • Gall, J. G., and H. G. Callan: H3-uridine incorporation in lampbrush chromosomes. Proc. nat. Acad. Sci. (Wash.) 48, 562–570 (1962).

    Google Scholar 

  • Gamborg, O. L., R. A. Miller, and K. Ojima: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158 (1968).

    Google Scholar 

  • Hsu, T. C.: Differential rate in RNA synthesis between euchromatin and heterochromatin. Exp. Cell Bes. 27, 332–334 (1962).

    Google Scholar 

  • Izawa, M., V. G. Allfrey, and A. E. Mirsky: The relationship between RNA synthesis and loop structure in lampbrush chromosomes. Proc. nat. Acad. Sci. (Wash.) 49, 544–551 (1963).

    Google Scholar 

  • Jacob, J., and J. L. Sirlin: Electron microscope studies on salivary gland cells. IV. The nucleus of Smittia parthenogenetica (Chironomidae) with special reference to the nucleolus and the effects of actinomycin thereon. J. Ultrastruct. Res. 11, 315–328 (1964).

    Google Scholar 

  • Laufer, H., Y. Nakase, and J. Vanderberg: Developmental studies on the dipteran salivary gland. I. The effect of actinomycin D on larval development, enzyme activity and chromosomal differentiation in Chironomus thummi. Develop. Biol. 9, 367–384 (1964).

    Google Scholar 

  • Littau, V. C., V. G. Allfrey, J. H. Frenster, and A. E. Mirsky: Active and inactive regions of nuclear chromatin as revealed by electron microscope autoradioraphy. Proc. nat. Acad. Sci. (Wash.) 52, 93–100 (1964).

    Google Scholar 

  • Lyon, M. F.: Chromosomal and subchromosomal inactivation. Ann. Rev. Genet. 2, 31–52 (1968).

    Google Scholar 

  • Mechelke, F.: Reversible Strukturmodifikationen der Speicheldrüsenchromosomen von Acricotopus lucidus. Chromosoma (Berl.) 5, 511–543 (1953).

    Google Scholar 

  • Meyer, G. F., u. O. Hess: Strukturdifferenzierungen im Y-Chromosom von Drosophila hydei und ihre Beziehungen zu Gen-Aktivitäten. II. Effekt der RNS-Synthese-Hemmung durch Actinomycin. Chromosoma (Berl.) 16, 249–270 (1965).

    Google Scholar 

  • Nagl, W.: Die SAT-Riesenchromosomen der Kerne des Suspensors von Phaseolus coccineus und ihr Verhalten während der Endomitose. Chromosoma (Berl.) 16, 511–520 (1965).

    Google Scholar 

  • —: Banded polytene chromosomes in the legume Phaseolus vulgaris. Nature (Lond.) 221, 70–71 (1969a).

    Google Scholar 

  • - Temperature-dependent functional structures of the polytene chromosomes in the suspensor of Phaseolus, with special reference to the nucleolus organizers. J. Cell Sci. 5 (in the press, 1969b).

  • Pelling, C.: Ribonukleinsäure-Synthese der Riesenchromosomen. Autoradiographische Untersuchungen an Chironomus tentans. Chromosoma (Berl.) 15, 71–122 (1964).

    Google Scholar 

  • Reich, E.: Actinomycin correlation of structure and function of its complexes with purines on DNA. Science 143, 684–689 (1964).

    Google Scholar 

  • Stevens, B. J.: The effect of actinomycin D on nucleolar and nuclear fine structure in the salivary gland cell of Chironomus thummi. J. Ultrastruct. Res. 11, 329–353 (1964).

    Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institut für Pflanzengenetik, Ladenburg am Neckar, Germany

    W. Nagl

  2. Botanisches Institut der Universität Wien, Rennweg 14, A-1030, Wien, Austria

    W. Nagl

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  1. W. Nagl
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Nagl, W. Correlation of structure and RNA synthesis in the nucleolus-organizing polytene chromosomes of Phaseolus vulgaris . Chromosoma 28, 85–92 (1969). https://doi.org/10.1007/BF00325992

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  • DOI: https://doi.org/10.1007/BF00325992

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