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Potyviruses, monoclonal antibodies, and antigenic sites

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Potyvirus Taxonomy

Part of the book series: Archives of Virology ((ARCHIVES SUPPL,volume 5))

Summary

Virus-specific and cross-reactive monoclonal antibodies have been produced to at least 19 different aphid-transmitted potyviruses. This report summarizes the development of these monoclonal antibodies as well as presents information on the delineation of the virus-specific and group-common epitopes defined by these monoclonal antibodies. Virus-specific and group-common antigenic determinants were mapped by a variety of techniques, including analysis of antigen: antibody reactivity patterns, determination of N-terminal vs. trypsin-resistant core peptide-specificity, immuno-analysis of overlapping synthetic peptides, and immunoanalysis of bacterially expressed coat-protein gene products. Of those monoclonal antibodies that have been examined, monoclonal antibody-defined virus-specific epitopes are virion surface-located within the 30+ amino acid amino terminus, whereas the group-common epitopes are found in the trypsin-resistant core protein not usually located on the virion surface, as has been shown previously with certain polyclonal antibodies. New information is presented on the analysis of bean yellow mosaic virus amino terminal epitopes as well as on the identification of amino terminal antigenic determinants shared between strains of bean yellow mosaic virus and pepper mottle virus. A recommendation on the evaluation and use of a panel of potyvirus broad-spectrum reacting monoclonal as reference monoclonal antibodies for the detection and classification of aphid-transmitted potyviruses is also presented.

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Author information

Authors and Affiliations

  1. Plant Sciences Institute, Florist and Nursery Crops Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland, USA

    R. Jordan

  2. BARC-West, FNCL, USDA-ARS, Building 004, Room 208, 10300 Baltimore Ave., Beltsville, MD, 20705-2350, USA

    R. Jordan

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  1. R. Jordan
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Editors and Affiliations

  1. Department of Plant Pathology and Physiology, Clemson University, Clemson, South Carolina, USA

    Ortus W. Barnett Jr.

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© 1992 Springer-Verlag

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Jordan, R. (1992). Potyviruses, monoclonal antibodies, and antigenic sites. In: Barnett, O.W. (eds) Potyvirus Taxonomy. Archives of Virology, vol 5. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6920-9_10

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  • DOI: https://doi.org/10.1007/978-3-7091-6920-9_10

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82353-8

  • Online ISBN: 978-3-7091-6920-9

  • eBook Packages: Springer Book Archive

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