Skip to main content
SpringerLink
Log in

Possible members of the potyvirus group transmitted by mites or whiteflies share epitopes with aphid-transmitted definitive members of the group

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

There are at least ten viruses identified in the literature that resemble definitive potyviruses in having flexuous filamentous particles and inducing the formation of “pinwheel” cytoplasmic inclusions in infected cells but that are transmitted by eriophyid mites, whiteflies or soil fungi and not by aphids, the vectors of the definitive potyviruses. The taxonomic status of these viruses is uncertain at present. Using a broadly cross-reactive antiserum raised against the dissociated coat protein core (residues 68–285) of a definitive potyvirus (Johnsongrass mosaic virus), we have shown that wheat streak mosaic virus which is transmitted by mite and sweet potato mild mottle virus which is transmitted by whitefly have coat proteins that share epitopes with definitive potyviruses. This finding further supports their classification as definitive members of the potyvirus group. The cross-reactive antiserum used here had been shown previously to react with coat proteins of fifteen different definitive potyviruses. The antiserum did not react with coat proteins of potexviruses and tobamoviruses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Allison RF, Johnston RE, Dougherty WG (1986) The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9–20

    Google Scholar 

  2. Batteiger B, Newhall WJ, Jones RB (1982) The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J Immunol Methods 55: 297–307

    Google Scholar 

  3. Brakke MK, Ball EM, Hsu YH, Langenberg WG (1987) Wheat streak mosaic virus cylindrical inclusion body protein. J Gen Virol 68: 281–287

    Google Scholar 

  4. Burgermeister W, Koenig R (1984) Electro-blot immunoassay—a means for studing serological relationships among plant viruses? Phytopathol Z 111: 15–25

    Google Scholar 

  5. Dietzgen RG, Francki RIB (1987) Nonspecific binding of immunoglobulins to coat proteins of certain plant viruses in immunoblots and indirect ELISA. J Virol Methods 15: 159–164

    Google Scholar 

  6. Domier LL, Franklin KM, Shahbuddin M, Hellman GM, Overmeyer JH, Hiremath ST, Siaw, MEE, Lomonossoff GP, Shaw JG, Rhoads E (1986) The nucleotide sequence of tobacco vein mottling virus. Nucleic Acids Res 14: 5417–5430

    Google Scholar 

  7. Fauquet C, Dejardin J, Thouvenel JC (1986) Evidence that the amino acid composition of the particle proteins of plant viruses is characteristic of the virus group. 1. Multidimensional classification of plant viruses. Intervirology 25: 1–13

    Google Scholar 

  8. Fauquet C, Dejardin J, Thouvenel JC (1986) Evidence that the amino acid composition of the particle proteins of plant viruses is characteristic of the virus group. 2. Discriminant analysis according to structural, biological and classification properties of plant viruses. Intervirology 25: 190–200

    Google Scholar 

  9. Francki RIB, Milne RG, Hatta T (1985) Atlas of plant viruses, vols 1 and 2. CRC Press, Boca Raton

    Google Scholar 

  10. Gershoni JM (1988) Protein blotting: a manual. Methods Biochem Anal 33: 1–58

    Google Scholar 

  11. Gibbs A (1986) Tobamovirus classification. In: Van Regenmortel HVM, Fraenkel-Conrat H (eds) The plant viruses: the rod-shaped plant viruses, vol 2. Plenum Press, New York, pp 167–180

    Google Scholar 

  12. Gough KH, Shukla DD (1981) Coat protein of potyviruses. 1. Comparison of the four Australian strains of sugarcane mosaic virus. Virology 111: 455–462

    Google Scholar 

  13. Harbison SA, Forster RLS, Guilford PJ, Gardner RC (1988) Organization and interviral homologies of the coat protein gene of white clover mosaic virus. Virology 162: 459–465

    Google Scholar 

  14. Hewish DR, Shukla DD, Gough KH (1986) The use of biotin conjugated antisera in immunoassays for plant viruses. J Virol Methods 13: 79–85

    Google Scholar 

  15. Hiebert E, Tremaine JH, Ronald WP (1984) The effect of limited proteolysis on the amino acid composition of five potyviruses and on the serological reaction and peptide maps of the tobacco etch virus capsid protein. Phytopathology 24: 411–416

    Google Scholar 

  16. Hollings M, Bock KR (1976) Sweet potato mild mottle virus. CMI/AAB Descript Plant Vir 162

  17. Hollings M, Brunt AA (1981) Potyviruses. In: Kurstak E (ed) Handbook of plant virus infections: comparative diagnosis. Elsevier/North-Holland, Amsterdam, pp 731–807

    Google Scholar 

  18. Johnson DA, Gautsch JW, Sportsman JR, Elder JH (1984) Improved technique utilizing non-fat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Gene Anal Tech 1: 3–8

    Google Scholar 

  19. Kaufmann SH, Ewing CM, Shaper JH (1987) The erasable Western blot. Anal Biochem 161: 89–95

    Google Scholar 

  20. Koenig R, Lesemann DE (1978) Potexvirus group. CMI/AAB Descript Plant Vir 200

  21. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680–685

    Google Scholar 

  22. Matthews REF (1982) Classification and nomenclature of viruses. Fourth report of the International Committee on Taxonomy of Viruses. Intervirology 17: 1–199

    Google Scholar 

  23. Morozov SYu, Lukasheva LI, Chernov BK, Skryabin KG, Atabekov JG (1987) Nucleotide sequence of the open reading frames adjacent to the coat protein cistron in potato virus X genome. FEBS Lett 213: 438–442

    Google Scholar 

  24. O'Donnell IJ, Shukla DD, Gough KH (1982) Electro-blot radioimmunoassay of virus-infected plant sap—a powerful new technique for detecting plant viruses. J Virol Methods 4: 19–26

    Google Scholar 

  25. Rubicki EP, Von Wechmar MB (1982) Enzyme-assisted immune detection of plant virus proteins electroblotted onto nitrocellulose paper. J Virol Methods 5: 267–278

    Google Scholar 

  26. Shukla DD, Inglis AS, McKern NM, Gough KH (1986) Coat protein of potyviruses. 2. Amino acid sequence of the coat protein of potato virus Y. Virology 152: 118–125

    Google Scholar 

  27. Shukla DD, Jilka D, Tosic M, Ford RE (1989) A novel approach to the serology of potyviruses involving affinity purified polyclonal antibodies directed towards virus-specific N-termini of coat proteins. J Gen Virol 70: 13–23

    Google Scholar 

  28. Shukla DD, McKern NM, Ward CW (1988) Coat protein of potyviruses. 5. Symptomatology, serology, and coat protein sequences of three strains of passionfruit woodiness virus. Arch Virol 102: 221–232

    Google Scholar 

  29. Shukla DD, Strike PM, Tracy SL, Gough KH, Ward CW (1988) The N and C termini of the coat proteins of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. J Gen Virol 69: 1497–1508

    Google Scholar 

  30. Shukla DD, Thomas JE, McKern NM, Tracy SL, Ward CW (1988) Coat protein of potyviruses. 4. Comparison of biological properties, serological relationships, and coat protein amino acid sequences of four strains of potato virus Y. Arch Virol 102: 207–219

    Google Scholar 

  31. Shukla DD, Tosic M, Jilka J, Ford RE, Toler RW, Langham MAC (1989) Taxonomy of potyviruses infecting maize, sorghum and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology 79: 223–229

    Google Scholar 

  32. Shukla DD, Ward CW (1988) Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. J Gen Virol 69: 2703–2710

    Google Scholar 

  33. Shukla DD, Ward CW (1989) Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group. Adv Virus Res 36: 273–314

    Google Scholar 

  34. Tovey ER, Ford SA, Baldo BA (1987) Protein blotting on nitrocellulose: some important aspects of the resolution and detection of antigens in complex extracts. J Biophys Biochem Methods 14: 1–17

    Google Scholar 

  35. Van Regenmortel MHV (1982) Serology and immunochemistry of plant viruses. Academic Press, New York

    Google Scholar 

  36. Veerisetty V (1979) Suggestions for the classification and nomenclature of helical plant viruses. Intervirology 11: 167–173

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biotechnology, Parkville Laboratory, CSIRO, Parkville, Victoria, Australia

    D. D. Shukla & C. W. Ward

  2. Department of Plant Pathology, University of Illinois, Urbana, Illinois, USA

    R. E. Ford

  3. Department of Biochemistry, University of Illinois, Urbana, Illinois, USA

    J. Jilka

  4. Faculty of Agriculture, University of Belgrade, Beograd-Zemun, Yugoslavia

    M. Tosic

Authors
  1. D. D. Shukla
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. E. Ford
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Tosic
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Jilka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. W. Ward
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shukla, D.D., Ford, R.E., Tosic, M. et al. Possible members of the potyvirus group transmitted by mites or whiteflies share epitopes with aphid-transmitted definitive members of the group. Archives of Virology 105, 143–151 (1989). https://doi.org/10.1007/BF01311352

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01311352

Keywords

Search

Navigation