Abstract
Fishes exhibit the greatest biodiversity among the vertebrates, making them an extremely attractive group to study a number of evolutionary questions. Over the last years, the development and improvement of cytogenetic FISH analyses have substantially expanded the methods of chromosome studies and have played an important role in the precise characterization of the structure of fish genomes. Here, besides presenting the current fish-FISH protocol, which is frequently applied in many laboratories for freshwater and marine species, we also include details about the isolation and preparation of the sequences most commonly used as probes in fish-FISH experiments. Moreover, considering the quality of the chromosomal preparations in fishes, some critical steps that are crucial for the success of the experiments are also highlighted.
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Acknowledgments
We wish to thank the Brazilian funding agencies FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo, Process 2014/23172-4) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Process 304992/2015-1) for the financial support.
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Yano, C.F., Bertollo, L.A.C., Cioffi, M.d.B. (2017). Fish-FISH: Molecular Cytogenetics in Fish Species. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_44
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DOI: https://doi.org/10.1007/978-3-662-52959-1_44
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