Abstract
DRβ sequence polymorphism has been analyzed using the polymerase chain reaction in vitro amplification method. The amplified DRβ segments have been cloned and sequenced to determine the extent of allotypic (allele) and isotypic (locus) variation. The sequences have been assigned to either the DRβ 1 or the second DRβ chain locus on specific haplotypes. This sequence information can serve as the basis for a rapid DNA typing method using a dot-blot format with sequence-specific oligonucleotide probes. The patchwork pattern of polymorphism revealed by this analysis may reflect gene conversion-like mechanisms between different alleles and different loci. A previously unreported sequence, termed DRβX, is described and helps define one of the three major evolutionary groups of DR haplotypes.
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Erlich, H.A., Scharf, S.J., Long, C.M., Horn, G.T. (1989). Analysis of Isotypic and Allotypic Sequence Variation in the HLA-DRβ Region Using the In Vitro Enzymatic Amplification of Specific DNA Segments. In: Dupont, B. (eds) Immunobiology of HLA. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-39946-0_50
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DOI: https://doi.org/10.1007/978-3-662-39946-0_50
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