Abstract
Trehalose is a disaccharide (α-d-glucopyranosyl α-d-glucopyranoside) commonly found in fungi and present at particularly high concentrations in resting cells and survival forms, such as spores and sclerotia. Two specific lines of research with respect to trehalose have received much attention. The first is in control of trehalose mobilization during the initiation of growth in resting cells and, more recently, the possible role of trehalose as a stress protectant. With respect to trehalose mobilization in fungi, two mechanisms have been proposed to trigger its onset, depending on the type of trehalase present in a particular species.
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References
Alabran DM, Ball DH, Reese ET (1983) Comparison of the trehalase of Trichoderma reesei with those from other sources. Carbohydr Res 123: 179–181
Anchordoguy TJ, Crowe JH, Griffin FJ, Clark WH (1988) Cryopreservation of sperm from the marine shrimp Sicyona engentis. Cryobiology 25: 238–243
App H, Holzer H (1989) Purification and characterization of neutral trehalase from the yeast ABYS1 mutant. J Biol Chem 264: 17583–17588
Arguelles JC, Gacto M (1985) Evidence for regulatory trehalase activity in Candida utilis. Can J Microbiol 31: 529–537
Arguelles JC, Gaeta M (1986) Comparative study of two trehalases from Candida utilis. Microbiologia 2: 105–114
Arguelles JC, Gacto M (1988) Differential location of regulatory and non-regulatory trehalases in Candida utilis cells. Antonie Leeuwenhoek 54: 555–565
Arguelles JC, Vicente-Soler J, Gacto M (1986) Protein phosphorylation and trehalase activation in Candida utilis. FEMS Microbiol Lett 34: 361–365
Attfield PV (1987) Trehalose accumulates in Saccharomyces cerevisiae during exposure to agents that induce heat shock response. FEBS Lett 225: 259–263
Attfield PV, Raman A, Northcott CJ (1992) Construction of Saccharomyces cerevisiae strains that accumulate relatively low concentrations of trehalose, and their application in testing the contribution of the disaccharide to stress tolerance. FEMS Microbiol Lett 94: 271–276
Barton JK, den Hollander JA, Hopfield JJ, Shulman RG (1982) 13C nuclear magnetic resonance study of trehalose mobilization in yeast spores. J Bacteriol 151: 177–185
Becher dos Passos J, Vanhalewyn M, Brandao RL, Castro IM, Nicoli JR, Thevelein JM (1992) Glucose-induced activation of plasma membrane HF-ATPase in mutants of the yeast Saccharomyces cerevisiae affected in cAMP metabolism, cAMP-dependent protein phosphorylation and the initiation of glycolysis. Biochim Biophys Acta 1136: 57–67
Becker JU, Shehata MI, Mizani SM (1982) Influence of nitrogen sources on glycogen metabolism in Saccharomyces carlsbergensis. J Gen Microbiol 128: 455–461
Belazzi T, Wagner A, Wieser R, Schanz M, Adam G, Hartig A, Ruis H (1991) Negative regulation of transcription of the Saccharomyces cerevisiae catalase T (CTTI) gene by cAMP is mediated by a positive control element. EMBO J 10: 585–592
Bell W, Klaassen P, Ohnacker M, Boller T, Herweijer M, Schoppink P, van der Zee P, Wiemken A (1992) Characterization of the 56kDa subunit of trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF], a regulator of carbon catabolite inactivation. Eur J Biochem 209: 951–959
Bhandal IS, Hauptmann RM, Widholm JM (1985) Trehalose as cryoprotectant for the freeze preservation of carrot and tobacco cells. Plant Physiol 78: 430–432
Bissinger PH, Wieser R, Hamilton B, Ruis H (1989) Control of Saccharomyces cerevisiae catalase T gene (CTT1) expression by nutrient supply via the RAS-cyclic AMP pathway. Mol Cell Biol 9: 1309–1315
Blakeley D, Tolliday B, Colaço C, Roser B (1990) Dry instant blood typing plate for bedside use. Lancet 336: 854–855
Blazquez MA, Stucka R, Feldmann H, Gancedo C (1992) Isolation in Schizosaccharomyces pombe of a homolog of the Saccharomyces cerevisiae CIF] gene. In: Worksh on control of gene expression in yeast. The Centre for International Meetings on Biology, Instituto Juan March de Estudios e Investigaciones 9: 60
Boiteux A (1992) Metabolic studies on synchronously dividing yeast cells. Energy metabolism during cellular division. Proc 10th Small Meet on Yeast — Transport and Energetics. Marburg, Germany, p 28
Boorstein WR, Craig EA (1990) Regulation of a yeast HSP70 gene by a cAMP responsive transcriptional control element. EMBO J 9: 2543–2553
Boos W, Ehmann U, Bremer E, Middendorf A, Postma P (1987) Trehalase of Escherichia coll. Mapping and cloning of its structural gene and identification of the enzyme as a periplasmic protein induced under high osmolarity growth conditions. J Biol Chem 262: 13212–13218
Bourret JA (1986) Evidence that a glucose-mediated rise in cyclic AMP triggers germination of Piloholus longipes spores. Exp Mycol 10: 60–66
Brana AF, Mendez C, Diaz LA, Manzanal MB, Hardisson C (1986) Glycogen and trehalose accumulation during colony development in Streptomyces antibioticus. J Gen Microbiol 132: 1319–1326
Breedveld MW, Zevenhuizen LPTM, Zehnder AJB (1991) Osmotically regulated trehalose accumulation and cyclic beta-(1,2)-glucan excretion by Rhizobium leguminosarum biovar trifolii TA-1. Arch Microbiol 156: 501506
Broach JR, Deschenes RJ (1990) The function of RAS genes in Saccharomyces cerevisiae. Adv Cancer Res 54: 79–139
Brownlee C, Jennings DH (1981) The content of soluble carbohydrates and their translocation in mycelium of Serpula lacrimans. Trans Br Mycol Soc 77: 615–619
Burke MJ (1985) The glassy state and survival of anhydrous biological systems. In: Leopold AC (ed) Membranes, metabolism and dry organisms. Cornell Univ Press, Ithaca, NY, pp 358–363
Cabib E, Leloir LF (1958) The biosynthesis of trehalose phosphate. J Biol Chem 231: 259–275
Callaerts G, Iserentant D, Verachtert H (1993) Relation between trehalose and sterol accumulation during oxygenation of cropped yeast. J Am Soc Brew Chem 51: 7577
Cameron S, Levin L, Zoller M, Wigler M (1988) cAMPindependent control of sporulation, glycogen metabolism, and heat shock resistance in S. cerevisiae. Cell 53: 555–566
Carrillo D, Vicente-Soler J, Gacto M (1992) Activation of neutral trehalase by fermentable sugars and cAMP in the fission yeast Schizosaccharomyces pombe. FEMS Microbial Lett 98: 61–66
Charlab R, Oliveira DE, Panek AD (1985) Investigation of the relationship between sstl and fdp mutations in yeast and their effect on trehalose synthesis. Braz J Med Biol Res 18: 447–454
Cherry JR, Johnson TR, Dollard C, Shuster JR, Denis CL (1989) Cyclic AMP-dependent protein kinase phosphorylates and inactivates the yeast transcriptional activator ADR1. Cell 56: 409–419
Clegg JS (1985) The physical properties and metalic status of Artemia cysts at low water contents: the “water replacement hypothesis”. In: Leopold AC (ed) Membranes, metabolism and dry organisms. Cornell Univ Press, Ithaca, NY, pp 169–187
Cochrane VW (1958) The physiology of fungi. Wiley, New York
Colaço C, Sen S, Thangavelu M, Pinder S, Roser B (1992) Extraordinary stability of enzymes dried in trehalose: simplified molecular biology. Biotechnology 10: 10071011
Coote PJ, Jones MV, Edgar K, Cole MB (1992) TPK gene products mediate cAMP-independent thermotolerance in Saccharomyces cerevisiae. J Gen Microbial 138: 25512557
Costa-Carvalho VLA, Panek AD, Rocha-Ledo MHM (1983) Glycogen accumulation by Saccharomyces cerevisiae: influence of specific growth rate. IRCS Med Sci 11: 120–121
Cotter DA (1975) Spores of the cellular slime mold Dictyostelium discoideum. In: Gerhardt P, Costilow RN, Sadoff HL (eds) Spores VI. Am Soc Microbiol, Washington, DC, pp 61–72
Coutinho C, Bernardes E, Felix D, Panek A (1988) Trehalose as cryoprotectant for preservation of yeast strains. J Biotechnol 7: 23–32
Coutinho CC, Silva JT, Panek AD (1992) Trehalase activity and its regulation during growth of Saccharomyces cerevisiae. Biochem Int 26: 521–530
Crowe JH, Crowe LM, Chapman D (1984) Preservation of membranes in anhydrobiotic organisms. Science 223: 701–703
Crowe JH, Carpenter JF, Crowe LM, Anchordoguy TJ (1990) Are freezing and dehydration similar stress vectors? A comparison of modes of interaction of stabilizing solutes with biomolecules. Cryobiology 27: 219–231
Crowe JH, Panek AD, Crowe LM, Panek AC, Dearaujo PD (1991) Trehalose transport in yeast cells. Biochem Int 24: 721–730
Crowe JH, Hoekstra FA, Crowe LM (1992) Anhydrobiosis. Annu Rev Physiol 54: 579–599
De Antoni GL, Perez P, Abraham A, Anon MC (1989) Trehalose, a cryoprotectant for Lactobacillus bulgaricus. Cryobiology 26: 149–153
De Araujo PS, Panek AC, Crowe JH, Crowe LM, Panek AD (1991) Trehalose-transporting membrane vesicles from yeasts. Biochem Int 24: 731–737
De Koning W, Groenveld K, Oehlen LJWM, Berden JA, Van Dam K (1991) Changes in the activities of key enzymes of glycolysis during the cell cycle in yeast: a rectification. J Gen Microbiol 137: 971–976
Dellamora-Ortiz GM, Ortiz CHD, Maia JCC, Panek AD (1986) Partial purification and characterization of the interconvertible forms of trehalase from Saccharomyces cerevisiae. Arch Biochem Biophys 251: 205–214
De Virgilio C, Simmen U, Hottiger T, Boller T, Wiemken A (1990) Heat shock induces enzymes of trehalose metabolism, trehalose accumulation, and thermotolerance in Schizosaccharomyces pombe, even in the presence of cycloheximide. FEBS Lett 273: 107–110
De Virgilio C, Bürckert N, Boller T, Wiemken A (199la) A method to study the rapid phosphorylation-related modulation of neutral trehalase activity by temperature shifts in yeast. FEBS Lett 291: 355–358
De Virgilio C, Muller J, Boller T, Wiemken A (1991b) A constitutive, heat shock-activated neutral trehalase occurs in Schizosaccharomyces pombe in addition to the sporulation-specific acid trehalase. FEMS Microbiol Lett 84: 85–90
De Virgilio C, Piper P, Boller T, Wiemken A (1991e) Acquisition of thermotolerance in Saccharomyces cerevisiae without heat shock protein hsp-104 and in the absence of protein synthesis. FEBS Lett 288: 86–90
De Virgilio C, Bürckert N, Bell W, Jenti B, Boller T, Wiemken A (1993) Disruption of TPS2, the gene encoding the 100 kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate phosphatase activity. Eur J Biochem 212: 315–323
Dewerchin MA, Van Laere AJ (1984) Trehalase activity and cyclic AMP content during early development of Mucor rounii spores. J Bacteriol 158: 575–579
Donnini C, Puglisi PP, Vecli A, Marmiroli N (1988) Germination of Saccharomyces cerevisiae ascospores without trehalose mobilization as revealed by in vivo 13C nuclear magnetic resonance spectroscopy. J Bacteriol 170: 37893791
Dumont JE, Jauniaux JC, Roger PP (1989) The cyclic AMP-mediated stimulation of cell proliferation. Trends Biochem Sci 14: 67–71
Elbein AD (1974) The metabolism of a-a-trehalase. Adv Carbohydr Chem Biochem 30: 227–256
Elliott B, Futcher B (1993) Stress resistance of yeast cells is largely independent of cell cycle phase. Yeast 9: 33–42
Emyanitoff RG, Wright BE (1979) Effect of intracellular carbohydrates on heat resistance of Dictyostelium descoideum spores. J Bacteriol 140: 1008–1012
Engelberg D, Perlman R, Levitzki A (1989) Transmembrane signalling in Saccharomyces cerevisiae. Cell Signalling 1: 1–7
Engelberg D, Poradosu E, Simchen E. Levitzki A (1990) Adenylyl cyclase activity of the fission yeast Schizosaccharomyces pombe is not regulated by guanyl nucleotides. FEBS Lett 261: 413–418
Farkas I, Hardy TA, Depaoliroach AA, Roach PJ (1990) Isolation of the GSYI gene encoding yeast glycogen synthase and evidence for the existence of a second gene. J Biol Chem 265: 20879–20886
Farkas I, Hardy TA, Goebl MG. Roach PJ (1991) Two glycogen synthase isoforms in Saccharomyces cerevisiae are coded by distinct genes that are differentially controlled. J Biol Chem 266:15602–15607
François J, Van Schaftingen E, Hers H-G (1984) The mechanism by which glucose increases frutose-2,6hisphosphate concentration in Saccharomyces cerevisiae. A cyclic-AMP-dependent activation of phosphofructokinase 2. Eur J Biochem 145: 187–193
François J, Eraso P, Gancedo C (1987) Changes in the concentration of cAMP, frutose-2,6-bisphosphate and related metabolites and enzymes in Saccharomyces cerevisiae during growth on glucose. Eur J Biochem 164: 369–373
François J, Neves M-J, Hers H-G (1991) The control of trehalose biosynthesis in Saccharomyces cerevisiae: evidence for a catabolite inactivation and repression of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase. Yeast 7: 575–587
Franks F. Hatley RHM, Mathias SF (1991) Materials science and the production of shelf-stable biologicals. Biopharm 4: 38–42
Fukui Y, Kozasa T, Kaziro Y, Takeda T, Yamamoto M (1986) Role of a ras homolog in the life cycle of Schizosaccharomyces pombe. Cell 44: 329–336
Gadd GM, Chalmers K. Reed RH (1987) The role of trehalose in dehydration resistance of Saccharomyces cerevisiae. FEMS Microbial Lett 48: 249–254
Gélinas P, Fiset G, LeDuy A, Goulet J (1989) Effect of growth conditions and trehalose content on cryotolerance of bakers’ yeast in frozen doughs. Appl Environ Microbiol 55: 2453–2459
Giver HM, Styrvold OB, Kaasen J. Strom AR (1988) Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli. J Bacteriol 170: 2841–2849
Gibbs JB, Marshall MS (1989) The ras oncogene — an important regulatory element in lower eucaryotic organisms. Microbial Rev 53: 171–185
Gonzalez MI. Stucka R, Blazquez MA, Feldmann H, Gancedo C (1992) Molecular cloning of CIFI,a yeast gene necessary for growth on glucose. Yeast 8:183192
Gottlieb D (1978) The germination of fungus spores. Merrow, Newcastle-upon-Tyne, Meadowfie Id Press, Shildon
Grba S, Oura E, Suomalainen H (1975) On the formation of glycogen and trehalose in baker’s yeast. Eur J Appl Microbiol 2: 29–37
Grba S, Oura E, Suomalainen H (1979) Formation of trehalose and glycogen in growing baker’s yeast. Finn Chem Lett 1979: 61–64
Green JL, Angell CA (1989) Phase relations and vitrification in saccharide-water solutions and the trehalose anomaly. J Phys Chem 93: 2880–2882
Gupta J, Harris SD, Cotter DA (1987) Evidence for nonregulatory trehalase activity in Dictyostelium discoideum. Curr Microbiol 16: 101–104
Gutierrez C, Ardourel M, Bremer E, Middendorf A, Boos W, Ehmann U (1989) Analysis and DNA sequence of the osmoregulated treA gene encoding the periplasmic trehalase of Escherichia coil K12. Mol Gen Genet 217: 347–354
Hall BG (1983) Yeast thermotolerance does not require protein synthesis. J Bacteriol 156: 1363–1365
Hammond JBW, Nichols R (1976) Carbohydrate metabolism in Agaricus bisporus (Lange) Sing.: changes in soluble carbohydrate during growth of mycelium and sporphore. J Gen Microbiol 95: 309–320
Harris DS, Cotter DA (1987) Vacuolar (lysosomal) trehalase of Saccharomyces cerevisiae. Curr Microbiol 15: 247–249
Hengge-Aronis R, Klein W, Lange R, Rimmele M, Boos W (1991) Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coll. J Bacteriol 173: 7918–7924
Hino A, Mihara K, Nakashima K, Takano H (1990) Trehalose levels and survival ratio of freeze-tolerant versus freeze-sensitive yeasts. Appl Environ Microbiol 56: 1386–1391
Hirimburegama K, Durnez P, Keleman J, Oris E, Vergauwen R, Mergelsberg H, Thevelein JM (1992) Nutrient-induced activation of trehalase in nutrient-starved cells of the yeast Saccharomyces cerevisiae: cAMP is not involved as second messenger. J Gen Microbiol 138: 2035–2043
Hohmann S, Huse K, Valentin E, Mbonyi K, Thevelein JM, Zimmermann FK (1992) Glucose-induced regulatory defects in the Saccharomyces cerevisiae growth initiation mutant bypl and identification of MIGI as a partial suppressor. J Bacteriol 174: 4183–4188
Hohmann S, Neves MJ, de Koning W, Alijo R, Ramos J, Thevelein JM (1993) The growth and signalling defects of the ggsl (fdpllbypl) deletion mutant on glucose are suppressed by a deletion of the gene encoding hexokinase PII. Curr Genet 23: 281–289
Honadel TE, Killian GJ (1988) Cryopreservation of murine embryos with trehalose and glycerol. Cryobiology 25: 331–337
Hottiger T, Boller T, Wiemken A (1987a) Rapid changes of heat and desiccation tolerance correlated with changes of trehalose content in Saccharomyces cerevisiae cells subjected to temperature shifts. FEBS Lett 220: 113–115
Hottiger T, Schmutz P, Wiemken A (1987b) Heat-induced accumulation and futile cycling of trehalose in Saccharomyces cerevisiae. J Bacteriol 169: 5518–5522
Hottiger T, Boller T, Wiemken A (1989) Correlation of trehalose content and heat resistance in yeast mutants altered in the RAS/adenylate cyclase pathway: is trehalose a thermoprotectant? FEBS Lett 255: 431434
Hottiger T, De Virgilio C, Bell W, Boller T, Wiemken A (1992) Canavanine treatment of yeast induces thermotolerance. Yeast 8 (Spec Iss): S91
Iida H (1988) Multistress resistance of Saccharomyces cerevisiae is generated by insertion of retrotransposon Ty into the 5’ coding region of the adenylate cyclase gene. Mol Cell Biol 8: 5555–5560
Iida H, Yahara I (1984) Specific early-G1 blocks accompanied with stringent response in Saccharomyces cerevisiae lead to growth arrest in resting state similar to the Go of higher eukaryotes. J Cell Biol 98: 1185–1193
Inoue H, Shimoda C (1981a) Changes in trehalose content and trehalase activity during spore germination in fission yeast, Schizosaccharomyces pombe. Arch Microhiol 129: 19–22
Inoue H, Shimoda C (1981 b) Induction of trehalase activity on a nitrogen-free medium: a sporulation-specific event in the fission yeast, Schizosaccharomyces pombe. Mol Gen Genet 183: 32–36
Jacquet M, Camonis 1 (1985) Contrôle du cycle de divisiion cellulaire et de la sporulation chez Saccharomyces cerevisiae par le système de I’AMP cyclique. Biochimie 67: 35–43
Kaasen I, Falkenberg P, Styrvold OB, Strom AR (1992) Moecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coll. Evidence that transcription is activated by katF (appR). J Bacteriol 174: 889–898
Keller F, Schellenberg M, Wiemken A (1982) Localization of trehalase in vacuoles and trehalose in the cytosol of yeast (Saccharomyces cerevisiae). Arch Microhiol 131: 298–301
Killick KA, Wright BE (1972a) Trehalose synthesis during differentiation in Dictyostelium discoideum. HI. In vitro unmaking of trehalose 6-phosphate synthetase. J Biol Chem 247: 2967–2969
Killick KA, Wright BE (1972b) Trehalose synthesis during differentiation in Dictyostelium discoideum. IV. Secretion of trehalase and in vitro expression of trehalose 6-phosphate synthetase activity. Biochem Biophys Res Commun 48: 1476–1481
Kline L, Sugihara TF (1968) Factors affecting the stability of frozen bread doughs. I. Prepared by the straight dough method. Bakers Dig 42: 44–50
Kobayashi N, McEntee K (1993) Identification of cis and trans components of a novel heat shock stress regulatory pathway in Saccharomyces cerevisiae. Mol Cell Biol 13: 248–256
Kopp M, Müller H, Holzer H (1993) Molecular analysis of the neutral trehalase gene from Saccharomyces cerevisiae. J Biol Chem 268: 4766–4774
Kotyk A, Michaljanicova D (1979) Uptake of trehalose by Saccharomyces cerevisiae. J Gen Microbiol 110: 323–332
Küenzi MT, Fiechter A (1969) Changes in carbohydrate composition and trehalase activity during the budding cycle of Saccharomyces cerevisiae. Arch Mikrobiol 64: 396–407
Küenzi MT, Fiechter A (1972) Regulation of carbohydrate composition of Saccharomyces cerevisiae under growth limitation. Arch Mikrobiol 84: 254–265
Levine H, Slade L (1992) Another view of trehalose for drying and stabilizing biological materials. Biopharm 5: 36–40
Lewis JG, Learmonth RP, Watson K (1993) Role of growth phase and ethanol in freeze-thaw stress resistance of Saccharomyces cerevisiae. Appl Environ Microhiol 59: 1065–1071
Lillie SH, Pringle JR (1980) Reserve carbohydrate metabolism in Saccharomyces cerevisiae. Response to nutrient limitation. J Bacteriol 143: 1384–1394
Londesborough J, Varimo K (1984) Characterization of two trehalases in baker’s yeast. Biochem J 219:511–518 Londesborough J, Vuorio 0 (1991) Trehalose-6-phosphate synthase/phosphatase complex from backers’ yeast: purification of a proteolytically activated form. J Gen Microbiol 137: 323–330
Ma H, Botstein D (1986) Effects of null mutations in the hexokinase genes of Saccharomyces cerevisiae on catabolite repression. Mol Cell Biol 6: 4046–4052
Mackay MA, Norton RS, Borowitzka LJ (1984) Organic osmoregulatory solutes in cyanobacteria. J Gen Microbiol 130: 2177–2191
Mackenzie KF, Singh KK, Brown AD (1988) Water stress plating hypersensitivity of yeasts: protective role of trehalose in Saccharomyces cerevisiae. J Gen Microbiol 134: 1661–1666
Mager WH, Moradas Ferreira P (1993) Stress response of yeast. Biochem J 290: 1–13
Malone RE (1990) Dual regulation of meiosis in yeast. Cell 61: 375–378
Marchler G, Schuller C, Wieser R, Adam G, Ruis H (1992) A heat shock factor-independent stress control element of the Saccharomyces cerevisiae CTTI promotor regulated by protein kinase A, nitrogen starvation and heat shock. Yeast 8 (Spec Iss): 5154
Marino C, Curto M, Bruno R, Rinaudo MT (1989) Trehalose synthase and trehalose behaviour in yeast cells in anhydrobiosis and hydrobiosis. Int J Biochem 21: 1369–1375
Martegani E, Baroni M, Vanoni M (1986) Interaction of cAMP with the CDC25-mediated step in the cell cycle of budding yeast. Exp Cell Res 162: 544–548
Martin MC, Diaz LA, Manzanal MB, Hardisson C (1986) Role of trehalose in the spores of Streptomyces. FEMS Microbiol Lett 35: 49–54
Matsumoto K, Uno I, Ishikawa T (1985) Genetic analysis of the role of cAMP in yeast. Yeast 1: 15–24
Mbonyi K, Van Aelst L, Argüelles JC, Jans AWH, Thevelein JM (1990) Glucose-induced hyperaccumulation of cAMP and absence of glucose repression in yeast strains with reduced activity of cAMP-dependent protein kinase. Mol Cell Biol 10: 4518–4523
McBride MJ, Ensign JC (1987a) Effects of intracellular trehalose content on Streptomyces griseus spores. J Bacteriol 169: 4995–5001
McBridge MJ, Ensign JC (1987b) Metabolism of endogenous trehalose by Streptomyces griseus spores and by spores or cells of other Actinomycetes. J Bacteriol 169: 5002–5007
McDougall J, Kaasen I, Strom AR (1993) A yeast gene for trehalose-6-phosphate synthase and its complementation of an Escherichia coil otsA mutant. FEMS Microbiol Lett 107: 25–30
Merritt PP (1960) The effect of preparation on the stability and performance of frozen, unbaked, yeast-leavened doughs. Bakers Dig 34: 57
Mittenbühler K, Holzer H (1988) Purification and characterization of acid trehalase from the yeast suc2 mutant. J Biol Chem 263: 8537–8543
Neves MJ, François J (1992) On the mechanism by which a heat shock induces trehalose accumulation in Saccharomyces cerevisiae. Biochem J 288: 859–864
Neves MJ, Jorge JA, François JM, Terenzi HF (1991) Effects of heat shock on the level of trehalose and glycogen. and on the induction of thermotolerance in Neurospora crassa. FEBS Lett 283: 19–22
Nikawa J, Cameron S, Toda T, Ferguson KW, Wigler M (1987) Rigorous feedback control of cAMP levels in Saccharomyces cerevisiae. Genes Dev 1: 931–937
Oda Y, Uno K, Ohta S (1986) Selection of yeasts for breadmaking by the frozen dough method. Appt Environ Microbiol 52: 941–943
Operti MS, Oliveira DE, Freitas-Valle AB, Oestreicher EG, Mattoon JR, Panek AD (1982) Relationships between trehalose metabolism and maltose utilization in Saccharomyces cerevisiae. Ill. Evidence for alternative pathways of trehalose synthesis. Curr Genet 5:69–76 Ortiz CH, Maia JCC, Tenan MN, Braz-Padrao GR, Mattoon JR, Panek AD (1983) Regulation of yeast trehalase by a monocyclic, cyclic AMP-dependent phosphorylation-dephosphorylation cascade system. J Bacteriol 153: 644–651
Otting G, Liepinsh E, Wuthrick K (1991) Protein hydration in aqueous solution. Science 254: 974–980
Padrao GRB, Malamud DR, Panek AD, Mattoon JR (1982) Regulation of energy metabolism in yeast. Inheritance of a pleiotropic mutation causing defects in metabolism of energy reserves, ethanol utilization and formation of cytochrome a.a3. Mol Gen Genet 185: 255261
Panek AD (1963) Function of trehalose in baker’s yeast (Saccharomyces cerevisiae). Arch Biochem Biophys 100: 422–425
Panek AD, Bernardes EJ (1983) Trehalose: its role in germination of Saccharomyces cerevisiae. Curr Genet 7: 393–397
Panek AC, de Araujo PS, Neto VM, Panek AD (1987) Regulation of the trehalose-6-phosphate synthase complex in Saccharomyces. Curr Genet 11: 459–465
Panek AD, Sampaio AL, Braz GC, Baker SJ, Mattoon JR (1980) Genetic and metabolic control of trehalose and glycogen synthesis. New relationships between energy reserves, catabolite repression and maltose utilization. Cell Mol Biol 25: 345–354
Panek AD, Ferreira R. Panek AC (1989) Comparative studies between the glucose-induced phosphorylation signal and the heat shock response in mutants of Saccharomyces cerevisiae. Biochimie 71: 313–318
Panek AC, Araujo PS, Poppe SC, Panek AD (1990) On the determination of trehalose-6-phosphate synthase in Saccharomyces. Biochem Int 21.695–704
Pardo LA, Sanchez SM, Lazo PS, Ramos S (1991) In vitro activation of the Saccharomyces cerevisiae Ras/ adenylate cyclase system by glucose and some of its analogues. FEBS Lett 290: 43–48
Parry JM, Davies PJ, Evians WE (1976) The effects of “cell age” upon the lethal effects of physical and chemical mutagens in the yeast Saccharomyces cerevisiae. Mol Gen Genet 146: 27–35
Paschoalin VMF, Costa-Carvalho VLA, Panek AD (1986) Further evidence for the alternative pathway of trehalose synthesis linked to maltose utilization in.Saccharomyces. Curr Genet 10: 725–731
Paschoalin VMF, Silva JT, Panek AD (1989) Identification of an ADPG-dependent trehalose synthase in Saccharomyces. Curr Genet 16: 81–87
Payen R (1949) Variation des teneurs en glycogène et en tréhalose pendant le séchage de la levure. Can J Res 27B: 749–756
Pillar TM, Bradshaw RE (1991) Heat shock and stationary phase induce transcription of the Saccharomyces cerevisiae iso-2-cytochrome c gene. Curr Genet 20: 185188
Piper PW, Lockheart A (1988) A temperature-sensitive mutant of Saccharomyces cerevisiae defective in the specific phosphatase of trehalose biosynthesis. FEMS Microbiol Lett 49: 245–250
Plesset J, Ludwig JR, Cox BS, McLaughlin CS (1987) Effect of cell cycle position on thermotolerance in Saccharomyces cerevisiae. J Bacteriol 169: 779–784
Pollock GE, Holmstrom CD (1951) The trehalose content and the quality of active dry yeast. Cereal Chem 28: 498505
Praekelt UM, Meacock PA (1990) HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function. Mol Gen Genet 223: 97–106
Reed RH, Borowitzka LJ, Mackay MA, Chudek JA, Forster R, Warr RSC, Moore DJ, Stewart WDP (1986) Organic solute accumulation in osmotically stressed cyanobacteria. FEMS Microbiol Rev 39: 51–56
Reed SI (1992) The role of p34 kinases in the G1 to S-phase transition. Annu Rev Cell Biol 8: 529–561
Rose M, Albig W, Entian KD (1991) Glucose repression in Saccharomyces cerevisiae is directly associated with hexose phosphorylation by hexokinase-PI and hexokinase-PII. Eur J Biochem 199: 511–518
Roser BJ (1991a) Trehalose drying: a novel replacement for freeze-drying. Biopharm 5: 44–53
Roser BJ (1991b) Trehalose, a new approach to premium dried foods. Trends Food Sci Technol 2: 166–169
Roth R (1970) Carbohydrate accumulation during the sporulation of yeast. J Bacteriol 101: 53–57
Roth R, Sussman M (1968) Trehalose 6-phosphate synthetase (uridine diphosphate glucose: D-glucose 6-phosphate 1-glucosyltransferase) and its regulation during slime mold development. J Biol Chem 243: 5081–5087
Rothman-Denes LB, Cabib E (1970) Two forms of yeast glycogen synthetase and their role in glycogen accumulation. Proc Natl Acad Sci USA 66: 967–974
Ruf J, Wacker H, James P, Maffia M, Seiler P, Galand G, von Kieckebusch A, Semenza G, Mantei N (1990) Rabbit small intestinal trehalase. Purification, cDNA cloning, expression, and verification of glycosylphosphatidylinositol anchoring. J Biol Chem 265: 15034–15039
Sacktor B (1968) Trehalase and the transport of glucose in the mammalian kidney and intestine. Proc Natl Acad Sci USA 60: 1007–1014
Sacktor B, Berger SJ (1969) Formation of trehalose from glucose in the renal cortex. Biochem Biophys Res Commun 35: 796–800
Saenger W (1989) Structure and dynamics of water surrounding biomolecules. Annu Rev Biophys Chem 16: 93114
Sanchez Y, Lindquist SL (1990) HSP104 required for induced thermotolerance. Science 248: 1112–1115
Sanchez Y, Taulien J, Borkovich KA, Lindquist S (1992) Hsp104 is required for tolerance to many forms of stress. EMBO J 11: 2357–2364
Schenberg-Frascino A, Moustacchi E (1972) Lethal and mutagenic effects of elevated temperature on haploid yeast. Mol Gen Genet 115: 243–257
Semenza G (1981) Intestinal oligo-and disaccharidases. In: Randle PJ, Steiner DF, Whelan WJ (eds) Carbohydrate metabolism and its disorders, vol 3. Academic Press, London, pp 425–479
Shin D-Y, Matsumoto K, Iida H, Uno 1, Ishikawa T (1987) Heat Shock Response of Saccharomyces cerevisiae mutants altered in cyclic AMP-dependent protein phosphorylation. Mol Cell Biol 7: 244–250
Slade L, Levine H (1988) Non-equilibrium behavior of small carbohydrate-water systems. Pure Appl Chem 60: 1841–1864
Smith SE (1967) Carbohydrate translocation in orchid mycorrhizas. New Phytol 66: 371–378
Stewart LC, Richtmeyer NK, Hudson CS (1950) The preparation of trehalose from yeast. J Am Chem Soc 72: 2059–2061
Strom AR, Falkenberg P, Landfald B (1986) Genetics of osmoregulation in Escherichia coli: uptake and biosynthesis of organic osmolytes. FEMS Microbiol Rev 39: 7986
Sugihara TF, Kline L (1986) Factors affecting the stability of frozen bread doughs. II. Prepared by the sponge and dough method. Bakers Dig 42: 51–54, 69
Sumida M, Ogura S, Miyata S, Arai M, Murao S (1989) Purification and some properties of trehalase from Chaetomium aureum MS — 27. J Ferment Bioeng 67: 8386
Suomalainen H, Pfäffii S (1961) Changes in the carbohydrate reserves of baker’s yeast during growth and on standing. J Inst Brew 67: 249–254
Sussman AS (1954) Changes in the permeabilty of ascospores of Neurospora tetrasperma during germination. J Gen Physiol 38: 59–77
Sussman AS, Halvorson HO (1966) Spores. Their dor- mancy and germination. Harper & Row, New York
Tanaka K, Matsumoto K, Toh-e A (1988) Dual regulation of the expression of the polyubiquitin gene by cyclic AMP and heat shock in yeast. EMBO J 7: 495–502
Tereshina VM, Polotebnova MV, Feofilova EP (1988) Trehalase activity of spores of the wild-type strain of Cunninghamella japonica and mutants with a reduced rate of trehalase synthesis. Microbiology 56: 587–592
Thevelein JM (1984a) Cyclic-AMP content and trehalase activation in vegetative cells and ascospores of yeast. Arch Microbiol 138: 64–67
Thevelein JM (1984b) Activation of trehalase by heat shock in yeast ascospores. Correlation with total cellular cyclic-AMP content. Curr Microbiol 10: 159–164
Thevelein JM (1984e) Regulation of trehalose mobilization in fungi. Microbiol Rev 48: 42–59
Thevelein JM (1988) Regulation of trehalase activity by phosphorylation-dephosphorylation during developmental transitions in fungi. Exp Mycol 12: 1–12
Thevelein JM (1991) Fermentable sugars and intracellular acidification as specific activators of the RAS adenylate cyclase signalling pathway in yeast — the relationship to nutrient induced cell cycle control. Mol Microbiol 5: 1301–1307
Thevelein JM (1992) The RAS-adenylate cyclase pathway and cell cycle control in Saccharomyces cerevisiae. In: Grivell L (ed) Molecular biology of yeasts. Antonie Leeuwenhoek, J Microbiology 62: 109–130, Kluwer, Dordrecht
Thevelein JM, Beullens M (1985) Cyclic AMP and the stimulation of trehalase activity in the yeast Saccharomyces cerevisiae by carbon sources, nitrogen sources and inhibitors of protein synthesis. J Gen Microbiol 131: 3199–3209
Thevelein JM, den Hollander JA, Shulman RG (1982) Changes in the activity and properties of trehalase during early germination of yeast ascospores: correlation with trehalose breakdown as studied by in vivo “C NMR. Proc Natl Acad Sci USA 79: 3503–3507
Toda T, Uno I, Ishikawa T, Powers S, Kataoka T, Broek D, Cameron S, Broach J, Matsumoto K, Wigler M (1985) In yeast, Ras proteins are controlling elements of adenylate cyclase. Cell 40: 27–36
Tripp ML, Paznokas JL (1982) Glucose-initiated germination of Mucor racemosus sporangiospores. J Gen Microbiol 128: 477–483
Trivedi NB, Jacobson G (1986) Recent advances in baker’s yeast. Prog Ind Microbiol 23: 45–71
Uno I, Matsumoto K, Adachi K, Ishikawa T (1983) Genetic and biochemical evidence that trehalase is a substrate of cAMP-dependent protein kinase in yeast. J Biol Chem 258: 10867–10872
Van Aelst L, Boy-Marcotte E, Camonis JH. Thevelein JM, Jacquet M (1990) the C-terminal part of the CDC25 gene product plays a key role in signal transduction in the glucose-induced modulation of cAMP level in Saccharomyces cerevisiae. Eur J Biochem 193: 675–680
Van Aelst L, Hohmann S, Zimmermann FK, Jans AWH, Thevelein JM (1991) A yeast homologue of the bovine lens fibre MIP gene family complements the growth defect of a Saccharomyces cerevisiae mutant on fermentable sugars but not its defect in glucose-induced RAS-mediated cAMP signalling. EMBO J 10: 2095–2104
Van Aelst L, Hohmann S, Bulaya B, De Koning W, Sierkstra L, Neves MJ, Luyten K, Alijo R, Ramos J, Coccetti P, Martegani E, de Magalhäes-Rocha NM, Brandäo RL, Van Dijck P, Vanhalewyn M, Durnez P, Jans AWH, Thevelein JM (1993) Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae. Mol Microbial 8: 927–943
van de Poll KW, Schamhart DHJ (1977) Characterization of a regulatory mutant of fructose-l,6-diphosphatase in Saccharomyces carlsbergensis. Mol Gen Genet 154: 6166
Vandercammen A, François J, Hers H-G (1989) Characterization of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Saccharomyces cerevisiae. Eur J Biochem 182: 613–620
Van der Plaat JB (1974) Cyclic 3’,5’-adenosine monophosphate stimulates trehalose degradation in backers’ yeast. Biochem Biophys Res Commun 56: 580–587
Van Dijck P, Colarizzon D, Smet P. Theelein JM (1995) Differential importance of trehalose in stress resistance in fermenting and nonfermenting Saccharomyces cerevisiae cells. Appt Environm Microbiol 61: 109–115
Van Doorn J, Scholte ME, Postma PW, van Driel R, van Dam K (1988a) Regulation of trehalase activity during the cell cycle of Saccharomyces cerevisiae. J Gen Microbiol 134: 785–790
Van Doom J, Valkenburg JAC, Scholte ME, Oehlen LJ, van Driel R, Postma PW, Nanninga N, van Dam K (1988b) Changes in activities of several enzymes involved in carbohydrate metabolism during the cell cycle of Saccharomyces cerevisiae. J Bacterial 170: 4808–4815
Vanhalewyn M, Thevelein JM (1992) Lcrl a mutation in the yeast adenylate cyclase gene. Yeast 8(Spec Iss):S391
Van Laere AJ (1986a) Biochemistry of spore germination in Phycomyces. FEMS Microbiol Rev 32: 189–198
Van Laere AJ (1986b) Resistance of germinating Phycomyces spores to desiccation, freezing, and acids. FEMS Microbiol Ecol 38: 251–256
Van Laere AJ (1989) Trehalose, reserve or stress metabolite. FEMS Microbiol Rev 63: 201–210
Van Laere A, Slegers LK (1987) Trehalose breakdown in germinating spores of Mucor rouxii. FEMS Microbiol Lett 41: 247–252
Van Laere A, François A, Overloop K, Verbeke M, Van Gerven L (1987) Relation between germination, trehalose and the status of water in Phycomyces blakeslceanus spores as measured by proton-NMR. J Gen Microbiol 133: 239–245
Van Mulders RM, Van Laere AJ (1984) Cyclic AMP, trehalase and germination of Phycomyces hlakesleeanus spores. J Gen Microbial 130: 541–547
Vicente-Soler J, Arguelles JC, Gacto M (1989) Presence of two trehalose-6-phosphate synthase enzymes in Candida utilis. FEMS Microbial Lett 61: 273–278
Vicente-Soler J. Arguelles JC, Gacto M (1991) Proteolytic activation of a,a-trehalose 6-phosphate synthase in Candida utilis. FEMS Microbial Lett 82: 157–161
Von Meyenburg HK (1969) Energetics of the budding cycle of Saccharomyces cerevisiae during glucose limited aerobic growth. Arch Mikrobiol 66: 289–303
Vuorio O, Londesborough J. Kalkkinen N (1992) Trehalose synthase: purification of the intact enzyme and cloning of the structural genes. Yeast 8 (Spec Iss): S626
Walton EF, Carter BLA, Pringle JR (1979) An enrichment method for temperature-sensitive and auxotrophic mutants of yeast. Mol Gen Genet 171: 111–114
Werner-Washburne M, Becker J, Kosic-Smithers J, Craig EA (1989) Yeast Hsp70 RNA levels vary in response to the physiological status of the cell. J Bacterial 171: 26802688
Wiemken A (1990) Trehalose in yeast, stress protectant rather than reserve carbohydrate. Anionic Leeuwenhock 58: 209–217
Wieser R, Adam G, Wagner A, Schuller C, Marchler G, Ruis H, Krawiec Z, Bilinski T (1991) Heat shock factor-independent heat control of transcription of the CTT1 gene encoding the cytosolic catalase-T of Saccharomyces cerevisiae. J Biol Chem 266: 12406–12411
Winkler K, Kienle I, Burgert M. Wagner JC, Holzer H (1991) Metabolic regulation of the trehalose content of vegetative yeast. FEBM Lett 291: 269–272
Yost HJ, Lindquist S (1991) Heat shock proteins affect RNA processing during the heat shock response of Saccharomyces cerevisiae. Mol Cell Biol 11: 1062–1068
Zevenhuizen LPTM (1992) Levels of trehalose and glycogen in Athrohacterglohiformis under conditions of nutrient starvation and osmotic stress. Anfonie Leeuwenhoek 61: 61–68
Zikmanis PB, Laivenieks MG, Auzinya LP, Kulaev IS, Beker ME (1985) Relationship between the content of high-molecular-weight polyphosphates and trehalose and viability of populations following dehydratation of the yeast Saccharomyces cerevisiae. Microbiology 54: 326–330
Zikmanis PB, Kruche RV, Auzinya LP, Margevicha MV, Becker E (1988) Distribution of trehalose between dehydrated Saccharomyces cerevisiae cells and the rehydratation medium. Microbiology 57: 414–416
Zimmermann ALS, Terenzi HF, Jorge JA (1990) Purification and properties of an extracellular conidial trehalase from Humicola grisea Var Thermoidea. Biochim Biophys Acta 1036: 41–46
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Thevelein, J.M. (1996). Regulation of Trehalose Metabolism and Its Relevance to cell Growth and Function. In: Brambl, R., Marzluf, G.A. (eds) Biochemistry and Molecular Biology. The Mycota, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-10367-8_19
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