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The Effect of Antigen Dose on the Changes in the Spleen: Tolerance versus Immunity

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Germinal Centers in Immune Responses

Abstract

In our laboratory we have been studying the effect of antigen dosage on the character and course of the immune response to soluble human serum albumin (HSA) in chickens. The primary response is fairly pronounced and allows not only the detection of various types of antibodies but also a quantitative follow-up of antibody-forming cells by means of immunofluorescence (Iványi et al., 1966; Černý and Iványi, 1966).

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References

  • Cerny, J., and J. Ivänyi: Antigen dose requirements for inhibition of the IgM and IgG antibody response in chickens. Studies on the cellular level. Folia biol. (Praha) 12, 343–354 (1966).

    CAS  Google Scholar 

  • Hanan, R., and J. Oyama: Inhibition of antibody formation in mature rabbits by contact with the antigen at an early age. J. Immunol. 73, 49–53 (1954).

    PubMed  CAS  Google Scholar 

  • Hanna, M. G., Jr.: An autoradiographic study of the germinal center in spleen white pulp during early intervals of the immune response. Lab. Invest. 13, 95–104 (1964).

    PubMed  Google Scholar 

  • Ivanyi, J., V. Valentova, and J. Cerny: The dose of antigen required for the supression of the IgM and IgG antibody response in chickens. I. The kinetics and characterization of serum antibodies. Folia biol. (Praha) 12, 157–167 (1966).

    CAS  Google Scholar 

  • Jerne, N., A. Nordin, C. Henry, H. Funi, and A. Koros: The agar plaque technique, the target of the antigen and clonal selection. Inform. Exch. Group No. 5, Immunopathology, Sci. memo 46 (1965).

    Google Scholar 

  • Leduc, E. H., A. H. Coons, and J. M. Connolly: Studies on antibody production. II. The primary and secondary response in the popliteal lymph node of the rabbits. J. exp. Med. 102, 61–71 (1955).

    Article  PubMed  CAS  Google Scholar 

  • Liacopoulos, P., and J. H. Goode: Transplantation tolerance induced in adult mice by protein overloading of donors. Science 146, 1305–1307 (1964).

    Article  PubMed  CAS  Google Scholar 

  • Makinodan, T, L. Hoppe, T. Sado, E. E. Capalbo, and M. R. Leonard: The supressive effect of supra-optimal dose of antigen on the secondary antibody-forming response of spleen cells cultured in cell-impermeable diffusion chambers. J. Immunol. 95, 466–479 (1965).

    PubMed  CAS  Google Scholar 

  • Perkins, A. H., and T Makinodan: Relative pool size of potentially competent antibody-forming cells of primed and non-primed spleen cells grown in in vivo culture. J. Immunol. 92, 192–200 (1964).

    PubMed  CAS  Google Scholar 

  • Stavitsky, A. B., and E. R. Arquilla: Micromethod for the study of protein and antibodies. III. Procedure and application of haemagglutination and haemagglutination-inhibition reaction with bisdiazotized benzidine and protein conjugated red blood cells. J. Immunol. 74, 306–317 (1955).

    PubMed  CAS  Google Scholar 

  • Urso, P., and T. Makinodan: The role of cellular division and maturation in the formation of precipitating antibody. J. Immunol. 90, 897–907 (1963).

    PubMed  CAS  Google Scholar 

  • Zaleski, M., J. öerny, and T. Hraba: Cytological changes in the regional lymph nodes of rabbits after local administration of human serum albumin. Folia biol. (Praha) 12, 22–28 (1966).

    CAS  Google Scholar 

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Černý, J., Viklický, V. (1967). The Effect of Antigen Dose on the Changes in the Spleen: Tolerance versus Immunity. In: Cottier, H., Odartchenko, N., Schindler, R., Congdon, C.C. (eds) Germinal Centers in Immune Responses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-86837-5_39

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  • DOI: https://doi.org/10.1007/978-3-642-86837-5_39

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-86839-9

  • Online ISBN: 978-3-642-86837-5

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