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Determination of Extra-and Intracellular pH Values in Relation to the Action of Acidic Gases on Cells

  • Chapter
Gases in Plant and Microbial Cells

Part of the book series: Modern Methods of Plant Analysis ((MOLMETHPLANT,volume 9))

Abstract

Gaseous air pollutants such as SO2 or NO2 are soluble in aqueous media. They react with water to form H2SO3, HNO2 and HNO3 which increase the concentration of H+ (or, as H+ does not really exist, that of the hydrogen-bonded complex H3O+ 3H2O) and thereby decrease pH, which is defined as the negative 10log of the proton concentration [H+]. For living cells the control of intracellular pH is essential for the maintenance of normal metabolic functions. The cytoplasmic pH of plant cells is usually close to 7.5 (Raven and Smith 1981). In chloroplasts, the stroma pH increases from about 7.5 to about 8 on illumination (Heldt et al. 1973; Oja et al. 1986), whereas the pH of the thylakoid compartment may drop to pH 5 owing to the transfer of protons from the stroma to the intrathylakoid space (Schuldiner et al. 1972). The large central vacuole of plant cells is usually acidic, with pH varying rather widely in different plant species (Smith and Raven 1979). In the cytoplasm, protons and hydroxyl ions are produced or consumed in many metabolic reactions (Raven 1985, 1986). Still, the internal pH is altered only within well-defined and rather narrow limits even when external pH values are very different (Langworthy 1978).

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  1. H. Pfanz
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  2. U. Heber
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  1. Goldberglein 7, D-8520, Erlangen, Germany

    Hans-Ferdinand Linskens

  2. Department of Biochemistry Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, SA, 5064, Australia

    John F. Jackson

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Pfanz, H., Heber, U. (1989). Determination of Extra-and Intracellular pH Values in Relation to the Action of Acidic Gases on Cells. In: Linskens, HF., Jackson, J.F. (eds) Gases in Plant and Microbial Cells. Modern Methods of Plant Analysis, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83346-5_18

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