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Liquid-Overlay Culture of Cellular Spheroids

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Spheroids in Cancer Research

Part of the book series: Recent Results in Cancer Research ((RECENTCANCER,volume 95))

Abstract

The aim of this work has been to develop realistic and easily handled models of tumor growth, from which increased knowledge of the physical and chemical factors that govern or sometimes disturb cell proliferation may be obtained. Emphasis has been laid mainly on the properties of cultured spheroids originating from mammalian cells. The three-dimensional arrangement affects the concentration of oxygen and other substances and invokes, for cells inside the spheriods, physical, chemical, and nutritional stress factors that are probably typical of poorly vascularized areas of solid tumors. Increased knowledge of this situation is important, because it is believed to determine radiation sensivity and also the effects of cytotoxic substances.

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Author information

Authors and Affiliations

  1. Department of Radiation Biology (FOA 4), National Defence Research Institute, 901 82, Umeå, Sweden

    J. Carlsson & J. M. Yuhas

Authors
  1. J. Carlsson
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  2. J. M. Yuhas
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Editor information

Editors and Affiliations

  1. Max-Plank-Institut für Systemphysiologie, Rheinlanddamm 201, 4600, Dortmund 1, Germany

    Helmut Acker

  2. Department of Radiobiology, National Defence Research Institut, 901 82, Umea, Sweden

    Jörgen Carlsson

  3. Medical Biophysics Unit, B. C. Cancer Research Center, 601 West 10th Avenue, Vancouver, BC, V5Z 1L3, Canada

    Ralph Durand

  4. Division Experimental Therapeutics, University of Rochester, Cancer Center, 601 Elmwood Avenue, Rochester, NY, USA

    Robert M. Sutherland

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© 1984 Springer-Verlag Berlin Heidelberg

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Carlsson, J., Yuhas, J.M. (1984). Liquid-Overlay Culture of Cellular Spheroids. In: Acker, H., Carlsson, J., Durand, R., Sutherland, R.M. (eds) Spheroids in Cancer Research. Recent Results in Cancer Research, vol 95. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82340-4_1

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  • DOI: https://doi.org/10.1007/978-3-642-82340-4_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-82342-8

  • Online ISBN: 978-3-642-82340-4

  • eBook Packages: Springer Book Archive

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