Zusammenfassung
Die Autoren beschreiben eine neue Methode der Suspensionskultur von Zellen. Sie beruht darauf, daß die Adhäsion der Zellen durch Benützung eines nicht-haftenden Agarsubstrats verhindert wird. Die nicht-haftenden Zellen aggregieren sich in Suspension. Morphologische Untersuchungen der Zellinseln zeigten Lebensfähigkeit und Proliferation. Es wird angenommen, daß die beschriebene Technik für die Austestung der Histokompatibilität verschiedener Zelltypen von Bedeutung ist, die zusammen kultiviert werden (z. B. Tumorzellen/Wirtszellen).
Summary
The authors describe a new method of cell suspension culture. It consists in preventing cell adhesion by using a non-adhesive agar substratum. The non-adhering cells are aggregating in suspension. The morphological study of these cell islands revealed viability and proliferation. It is assumed that the described technique may be of importance in testing histocompatibility of different types of cells cultivated together (e. g. tumor cells/host cells).
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Costăchel, O., Fadei, L. & Badea, E. Tumor cell suspension culture on non adhesive substratum. Z Krebs-forsch 72, 24–31 (1969). https://doi.org/10.1007/BF00524788
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DOI: https://doi.org/10.1007/BF00524788