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Cryopreservation of Embryos

  • Conference paper
Reproductive Biology and Plant Breeding

Abstract

For the last decade, plant cell cultures have become subjects of intensive research with emphasis on important biotechnological applications such as large scale production of clonal plants, genetic engineering, production and isolation of mutants, selection of variants, screening for resistance to various stresses and creation of new plant species through somatic cell hybridization. In this frame, somatic embryogenesis is widely used, due to its numerous advantages. However, this development leads to the production of an increasing number of strains. This creates problems for the management of laboratories. Moreover, many strains are faced with somaclonal variation and progressive loss of organogenic potentialities. Finally, genetically engineered lines are of extreme value, due to the amount of work necessary for their development. Therefore, safe and efficient techniques are needed for the long term conservation of this new germplasm.

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References

  • Bajaj YPS (1983) Cryopreservation of germplasm of cereals - Progress and prospects. Proc 6th Intl Wheat Genet Symp Kyoto Japan: 565–574

    Google Scholar 

  • Bajaj YPS (1983) Cryopreservation of germplasm of cereals - Progress and prospects. Proc 6th Intl Wheat Genet Symp Kyoto Japan: 565–574

    Google Scholar 

  • Bertrand-Desbrunais A (1991) La conservation des ressources génétiques des caféiers. Thèse de Doctorat Université Paris 6: 259 p

    Google Scholar 

  • Bertrand-Desbrunais A, Fabre J, Engelmann F, Dereuddre J, Charrier A (1988) Reprise de l’embryogenèse adventive d’embryons somatiques de cafeier (Coffea arabica) après leur congélation dans l’azote liquide. C R Acad Sci Paris 307 Sér III: 795–801

    Google Scholar 

  • de Boucaud MT, Brison M (1991) Cryopreservation of embryonic axes of Prunus persica L Batsch. Proc Cryo 91, KU Leuven, 7–12 july 1991: 102.

    Google Scholar 

  • de Boucaud MT, Brison M, Ledoux C, Germain E, Lutz A (1991) Cryopreservation of embryonic axes of recalcitrant seed: Juglans regia L cv Franquette. Cryo-Lett 12: 163–166

    Google Scholar 

  • Chin HF, Krishnapillay B, Alang ZC (1988) Cryopreservation of Veitchia and Howea palm embryos: non-development of the haustorium. Cryo-Lett 9: 372–379

    Google Scholar 

  • Chin HF, Krishnapillay B, Hor YL (1989) A note on the cryopreservation of embryos from young coconuts (Cocos nucifera var Mawa). Pertanika 12: 183–186

    Google Scholar 

  • Delvallée I, Guillaud J, Beckert M, Dumas C (1989) Cryopreservation of immature maize embryos after freeze-hardening in the ear and in vitro. Plant Sci 60: 129–136

    Article  Google Scholar 

  • Dereuddre J, Engelmann F (1987) The use of cryopreservation for setting up banks of plant germplasm. Proc Coll Franco-Britannique IAPTC Angers 8–9 oct 1987: 48–78

    Google Scholar 

  • Engelmann F (1986) Cryoconservation des embryons somatiques de palmier à huile (Elaeis guineensis Jacq). Mise au point des conditions de survie et de reprise. Thèse de Doctorat Université Paris 6: 228 p

    Google Scholar 

  • Engelmann F (1991) Le développement actuel de la cryoconservation des embryons somatiques de palmier à huile. Proc XVIIIB Cong Intl du Froid, Montréal, 10–17 aout 1991: 305

    Google Scholar 

  • Engelmann F (in press) In vitro conservation of tropical plant germplasm - a review. Euphytica: in press

    Google Scholar 

  • Engelmann F, Assy-Bah B (1991) Maintenance of coconut genetic resources - In vitro techniques for medium and long term conservation. Proc Intl Workshop on Coconut Genetic Resources Cipanas Indonesia 8–11 oct 1991: in press

    Google Scholar 

  • Engelmann F, Dereuddre J (1988a) Effets du milieu de culture sur la production d’embryoldes destinés à la cryoconservation chez le palmier à huile (Elaeis guineensis Jacq). C R Acad Sci Paris 306 Sér III: 515–520

    Google Scholar 

  • Engelmann F, Dereuddre J. (1988b) Cryopreservation of oil palm somatic embryos: importance of the freezing process. Cryo-Lett 7: 220–235

    Google Scholar 

  • Engelmann F, Duval Y, Dereuddre J (1985) Survie et prolifération d’embryons somatiques de Palmier à huile (Elaeis guineensis Jacq) après congélation dans l’azote liquide. C R Acad Sci Paris 301 Ser III: 111–116

    Google Scholar 

  • Florin B (1989) Etude de différentes voies de conservation d’embryons, de tissus et de cellules de végétaux cultivés in vitro: applications de l’hypoxie et de la cryoconservation. Thèse de Doctorat, Université de Tours: 259 p

    Google Scholar 

  • Grout BWW, Shelton K, Pritchard HIV (1983) Orthodox behaviour of oil palm seed and cryopreservation of the excised embryo for genetic conservation. Ann Bot 52: 381–384

    Google Scholar 

  • Kartha KK (ed) (1985) Cryopreservation of Plant Cells and Organs. CRC Press Boca Raton Florida

    Google Scholar 

  • Marin ML, Duran-Vila N (1988) Survival of somatic embryos and recovery of plants of sweet orange (Citrus sinensis L Osb) after immersion in liquid nitrogen. Plant Cell Tissue Organ Cult 14: 51–57

    Article  Google Scholar 

  • Marin ML, Mafia G, Roca WM, Withers LA (1990) Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen. Cryo-Lett 11: 257–264

    Google Scholar 

  • Monnier M, Leddet C (1980) Action du saccharose sur la résistance au gel des embryons immatures de capselle. Bull Soc Bot Fr 127: 71–77

    Google Scholar 

  • More A, Abdelnour A, Villalobos V (1991) Cryopreservation of Musa zygotic embryos. Proc 4th IPBNet Conf Biotechnology for Tropical Crop Improvement in Latin America San Jose Costa Rica 14–18 january 1991: 81

    Google Scholar 

  • Mycock DJ, Berjak P, Blakeway FC, Watt P (1989) Development of a model system for cryostorage: applicability to recalcitrant material. Proc Intl Conf The Impact of Biotechnology in Agriculture Amiens France 10–12 july 1989: CP1

    Google Scholar 

  • Normah MN, Chin HF, Hor YL (1986) Desiccation and cryopreservation of embryonic axes of Hevea brasiliensis Muell–Arg. Pertanika 9: 299–303

    Google Scholar 

  • Pence VC, Dresser BL (1988) Embryo cryostorage as a technique for germplasm preservation of several large-seeded tree species. Abst Beltsville Symp in Agric Res XIII Biotic Diversity and Germplasm Preservation - Global Imperatives 9–11 may 1988: 24

    Google Scholar 

  • Pritchard HW, Prendergast FG (1986) Effects of desiccation on the in vitro viability of embryos of the recalcitrant seed species Araucaria hunsteinii K Schum. J Plant Physiol 37: 1388–1397

    Google Scholar 

  • Sudarmonowati E, Henshaw GG (1990) Cryopreservation of cassava somatic embryos. Abstr VIIth Intl Cong Plant Tissue and Cell Culture, Amsterdam: 378

    Google Scholar 

  • Uragami A, Sakai A, Nagai M (1990) Cryopreservation of dried axillary buds from plantlets of Asparagus officinalis L grown in vitro. Plant Cell Rep 9: 328–331

    Article  Google Scholar 

  • Withers LA (1979) Freeze preservation of somatic embryos and clonal plantlets of carrot (Daucus carota). Plant Physiol 63: 460–467

    Article  PubMed  CAS  Google Scholar 

  • Withers LA (1982) The development of cryopreservation techniques for plant cell, tissue and organ culture. Proc 5th Intl Congr Plant Cell Tissue Culture Fujiwara A Ed Tokyo: 793–794

    Google Scholar 

  • Zavala ME, Sussex IM (1986) Survival of developing wheat embryos and bean axes following cryoprotection and freezing in liquid nitrogen. J Plant Physiol 122: 193–197

    CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales, ORSTOM (Institut Français de Recherche Scientifique pour le Développement en Coopération), 911 Av. Agropolis, BP 5045, 34032, Montpellier Cédex 01, France

    F. Engelmann

Authors
  1. F. Engelmann
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Editor information

Editors and Affiliations

  1. La Minière, G.E.V.E.S., 78285, Guyancourt Cédex, France

    Yvette Dattée

  2. RCAP INRA 23879, Université Lyon I, Bât 741, 43 boulevard du 11 Novembre, 69622, Villeurbanne Cédex, France

    Christian Dumas

  3. Station de Génétique végétale, I.N.A.-P.G., Ferme du Moulon, 91190, Gif-sur-Yvette, France

    André Gallais

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© 1992 Springer-Verlag Berlin Heidelberg

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Engelmann, F. (1992). Cryopreservation of Embryos. In: Dattée, Y., Dumas, C., Gallais, A. (eds) Reproductive Biology and Plant Breeding. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76998-6_27

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  • DOI: https://doi.org/10.1007/978-3-642-76998-6_27

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77000-5

  • Online ISBN: 978-3-642-76998-6

  • eBook Packages: Springer Book Archive

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