Abstract
Our lab has long studied the action of translational repressors (Miller et al., 1985; Andrake et al., 1988; Tuerk et al., 1990; McPheeters et al., 1988) and, more recently, a bacteriophage T4-encoded, Shine and Dalgarno-specific endonuclease (Ruckman et al., 1989). We were concerned with mechanisms for controlling the yield of protein from a given amount of mRNA; repressors provide reversible diminution of yield, while the endonuclease provides irreversible cessation of translation initiation on mRNAs that have been torn from their Shine and Dalgarno. When we invented TOEPRINTING (Hartz et al., 1988), we used that assay to further study repression (Winter et al., 1987; McPheeters et al., 1988; Unnithan et al., submitted). However, we quickly understood that we could contribute to a basic understanding of the unregulated initiation pathway.
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References
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Gold, L., Hartz, D. (1990). Initiation of Protein Synthesis in E. Coli: The Two Crucial Steps. In: McCarthy, J.E.G., Tuite, M.F. (eds) Post-Transcriptional Control of Gene Expression. NATO ASI Series, vol 49. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75139-4_40
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DOI: https://doi.org/10.1007/978-3-642-75139-4_40
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