Abstract
The sequence analysis of proteins by the stepwise removal of amino acids from the amino-terminus by Edman chemistry has been widely used for over thirty five years. The method utilizes phenylisothiocyanate and produces phenylthiohydantoin derivatives of amino acids. The sensitivity of the method has progressed from the micromole to the nanomole to the picomole range over the same period of time. One of the strengths of the method is the ability to sequence proteins through 30–50 residues with repetitive yields of 90–98%. A second advantage is that cycle times of 30–60 minutes allow the analysis of one to two samples per day.
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© 1989 Springer-Verlag Berlin Heidelberg
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Miller, C.G., Shively, J.E. (1989). Carboxyl-terminal Sequence Analysis of Proteins and Peptides by Chemical Methods. In: Wittmann-Liebold, B. (eds) Methods in Protein Sequence Analysis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73834-0_19
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DOI: https://doi.org/10.1007/978-3-642-73834-0_19
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-73836-4
Online ISBN: 978-3-642-73834-0
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