Abstract
The chromosome from prokaryotic organisms can be isolated in a packaged form termed the nucleoid (Stonington and Pettijohn 1971). The nucleoid is obtained either as membrane-free or membrane-associated depending on the type of detergent and ionic strength used when lysing the cells (Worcel and Burgi 1972; Pettijohn 1976). Studies with in vitro high-salt nucleoids isolated from E. coli have shown that the DNA in these particles is separated into a number of superhelical domains and that they contain an RNA-protein core. The protein components of the high-salt nucleoid include the ββ′ and α subunits of RNA-polymerase, the major outer membrane proteins of E. coli and a 56 kD protein (Portalier and Worcel 1976). Two acid extractable Polypeptides with molecular weights of 10 and 17 kD are enriched in the membrane-associated compared to the membrane-free nucleoid (Varshavsky et al. 1977).
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References
Abe M, Brown C, Hendrickson WG, Boyd DH, Clifford P, Cote RH, Schaechter M (1977) Release of Escherichia coli DNA from membrane complexes by single-strand endonucleases. Proc Natl Acad Sci USA 74:2756–2760
Aasland R, Hoick A, Lossius I, Haarr L, Kleppe K (1985) Proteins associated with the Escherichia coli chromosome (to be published)
Bjornsti MA, Hobot JA, Kelus AS, Villiger W, Kellenberger E (1985) Immunocytochemical localization of protein and DNA compounds of the bacterial nucleoid. Symp Chromatin Struct Funct. Camerino 1985, pp 161–162
Chrysogelos S, Griffith J (1982) Escherichia coli single-strand binding protein organizes single-stranded DNA in nucleosome-like units. Proc Natl Acad Sci USA 79:5803–5807
Craine BL, Rupert CL (1979) Deoxyribonucleic acid-membrane interactions near the origin of replication and initiation of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol 137:740–745
Cukier-Kahn R, Lacquet M, Gros F (1972) Two heat-resistant, low molecular weight proteins from Escherichia coli that stimulate DNA-directed RNA synthesis. Proc Natl Acad Sci (USA) 69: 3643–3647
Dixon NE, Kornberg A (1984) Protein HU in the enzymatic replication of the chromosomal origin of Escherichia coli. Proc Natl Acad Sci USA 81:424–428
Dworsky P, Schaechter M (1973) Effect of rifampin on the structure and membrane-attachment of the nucleoid of Escherichia coli. J Bacteriol 116:1365–1374
Georgiev GP, Bakayev W, Nedospasov SA, Razin SV, Matieva VL (1981) Studies on structure and function of chromatin. Mol Cell Biochem 40:29–48
Griffith J (1976) Visualization of procaryotic DNA in regularly condensed chromatin-like fiber. Proc Natl Acad Sci USA 63:563–567
Hoick A, Kleppe K (1985) Affinity of protein HU for different nucleic acids. FEBS Lett 185:121–124
Hübscher U, Lutz H, Kornberg A (1980) Novel histone H2A-like protein of Escherichia coli. Proc Natl Acad Sci USA 77:5097–5101
Kleppe K, Øvrebø S, Lossius I (1979) The bacterial nucleoid. J Gen Microbiol 112:1–13
Kleppe K, Lossius I, Aasland R, Sjåstad K, Hoick A, Haarr L (1984) The structure of the bacterial nucleoid. In: Hübscher U, Spadari S (eds) Proteins involved in DNA replication. Plenum, New York, pp 457–466
Lathe R, Buc H, Lecocq JP, Bautz EKF (1980) Prokaryotic histone-like protein interacting with RNA-polymerase. Proc Natl Acad Sci USA 77:3548–3552
Le Hegarat F, Salti V, Hirschbein L (1985) Symp Chromatin Struct Funct. Camerino 1985, p 163
Lossius I, Krüger PG, Kleppe K (1981) Effect of methyl methanesulphonate on the nucleoid structure of Escherichia coli. J Gen Microbiol 124:159–171
Lossius I, Krüger PG, Male R, Kleppe K (1983) Mitomycin-C induced changes in the nucleoid of Escherichia coli K12. Mutat Res 109:13–20
Lossius I, Sjåstad K, Haarr L, Kleppe K (1984) Two dimensional gel-electrophoretic separation of the proteins present in chromatin of Escherichia coli. J Gen Microbiol 130:3153–3157
Lossius I, Aasland R, Hoick A, Sjåstad K, Kleppe K (1985a) Analysis of micrococcal nuclease digestion products of Escherichia coli chromatin by isokinetic and isopycnic gradient centrifugation (Submitted for publication)
Lossius I, Sjåstad K, Haarr L, Kleppe K (1985b) Effect of mitomycin-C on the proteins associated with Escherichia coli chromatin (in preparation)
Marshall T, Latner AL (1981) Incorporation of methylamine in an ultrasensitive silver stain for detecting protein in thick Polyacrylamide gels. Electrophoresis 2:228–235
Pettijohn DE (1976) Prokaryotic DNA in nucleoid structure. CRC Crit Rev Biochem 4:175–202
Portalier R, Worcel A (1976) Association of the folded chromosome with the cell envelope of Escherichia coli: Characterization of the proteins at the DNA-attachment site. Cell 8:245–255
Rouviere-Yaniv J (1978) Localization of the HU protein on the Escherichia coli nucleoid. Cold Spring Harbour Symp Quant Biol 42:439–447
Rouviere-Yaniv J, Gros F (1975) Characterization of a novel low-molecular-weight DNA-binding protein from Escherichia coli. Proc Natl Acad Sci USA 72:3428–3432
Rouviere-Yaniv J, Yaniv M, Germond J-E (1979) Escherichia coli DNA binding protein HU form a nucleosome-like structure with circular double-stranded DNA. Cell 17:265–274
Sinden RR, Pettijohn DE (1981) Chromosomes in living Escherichia coli cells are segregated into domains of supercoiling. Proc Natl Acad Sci USA 78:224–228
Sjåstad K, Fadnes P, Krüger PG, Lossius I, Kleppe K (1982) Isolation, properties and nucleolytic degradation of chromatin from Escherichia coli. J Gen Microbiol 128:3037–3050
Sjåstad K, Haarr L, Kleppe K (1983) Characterization of the DNA-cellulose-binding proteins from Escherichia coli. Biochem Biophys Acta 739:8–16
Snyder M, Drlica K (1979) DNA gyrase on the bacterial chromosome: DNA cleavage induced by oxolinic acid. J Mol Biol 131:287–302
Sloof P, Magdelijn A, Boswinkel E (1983) Folding of prokaryotic DNA. Isolation and characterization of nucleoids from Bacillus licheniformis. J Mol Biol 163:277–297
Stonington OG, Pettijohn DE (1971) The folded genome of Escherichia coli isolated in a protein DNA-RNA complex. Proc Natl Acad Sci USA 68:6–9
Suryanarayana R, Subramanian A-R (1978) Specific association of two homologous DNA-binding proteins to the native 30-S ribosomal subunits of Escherichia coli. Biochem Biophys Acta 520: 342–357
Varshavsky AJ, Nedospasov A, Bakayev W, Bakayeva TG, Georgiev GP (1977) Histone like proteins in the purified Escherichia coli deoxyribonucleoprotein. Nucleic Acids Res 4:2725–2745
Worcel A, Burgi E (1972) On the structure of the folded chromosome of Escherichia coli. J Mol Biol 71:127–147
Worcel A, Burgi E (1974) Properties of a membrane-attached form of the folded chromosome of Escherichia coli. J Mol Biol 82:91–105
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© 1986 Springer-Verlag Berlin Heidelberg
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Lossius, I., Holck, A., Aasland, R., Haarr, L., Kleppe, K. (1986). Proteins Associated with Chromatin from Escherichia coli . In: Gualerzi, C.O., Pon, C.L. (eds) Bacterial Chromatin. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71266-1_9
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DOI: https://doi.org/10.1007/978-3-642-71266-1_9
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