Bacterial Chromatin

1. Front Matter

   Pages I-IX

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2. What is the Logic of DNA Packing in Bacteria?

   • P. Woolley

   Pages 1-10

3. Considerations on the Condensation and the Degree of Compactness in Non-Eukaryotic DNA-Containing Plasmas

   • E. Kellenberger, E. Carlemalm, J. Sechaud, A. Ryter, G. De Haller

   Pages 11-25

4. DNA-Binding Proteins and Their Interactions with Structure-Building Ions

   • G. Geraci, L. Tomei, V. Calabro, G. Quagliarotti, L. De Petrocellis

   Pages 26-29

5. The Packaging of DNA in Bacteriophage Lambda

   • J. Welsh, C. R. Cantor

   Pages 30-44

6. The Association of the Escherichia coli Chromosome with the Cell Membrane

   • G. B. Ogden, M. Schaechter

   Pages 45-51

7. DNA Topoisomerase Mutations in Bacteria

   • K. Drlica, G. J. Pruss, S. H. Manes, S. G. Chevalier

   Pages 52-63

8. New Electron Microscopic Data on the Structure of the Nucleoid and Their Functional Consequences

   • M. A. Bjornsti, J. A. Hobot, A. S. Kelus, W. Villiger, E. Kellenberger

   Pages 64-81

9. Detection and Identification of Proteins Cross-Linked in Vivo to Nucleic Acids

   • J. P. Schouten

   Pages 82-90

10. Proteins Associated with Chromatin from Escherichia coli

    • I. Lossius, A. Holck, R. Aasland, L. Haarr, K. Kleppe

    Pages 91-100

11. Proteins from the Prokaryotic Nucleoid. Structural and Functional Characterization of the Escherichia coli DNA-Binding Proteins NS (HU) and H-NS

    • C. O. Gualerzi, M. A. Losso, M. Lammi, K. Friedrich, R. T. Pawlik, M. A. Canonaco et al.

    Pages 101-134

12. Proteins from the Prokaryotic Nucleoid. [¹H]-NMR Studies on Escherichia coli Histone-like Proteins NS and H-NS and Their Interaction with DNA

    • M. Paci, C. L. Pon, M. A. Canonaco, C. O. Gualerzi

    Pages 135-154

13. Characterization of Bacillus subtilis Nucleoid DNA-Binding Proteins

    • F. Le Hégarat, V. Salti, L. Hirschbein

    Pages 155-166

14. Escherichia coli Protein H1 a Strongly Compacts DNA in Vitro

    • S. Rimsky, A. Spassky

    Pages 167-174

15. The Archaebacterial Histone “HTa”

    • D. G. Searcy

    Pages 175-184

16. The Structure of DNA-Binding Proteins from Eu- and Archaebacteria

    • J. Dijk, R. Reinhardt

    Pages 185-218

17. Nachwort: Converging Parallels

    • Claudio O. Gualerzi, Cynthia L. Pon

    Pages 219-219

18. Back Matter

    Pages 223-227

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This book brings together various contributions aimed at the elucida­ tion of the structural and functional organization of the bacterial nucleoid. Most of these papers, spanning the fields of physical chemistry through biochemistry to genetics, were presented at the session on bac­ terial chromatin during the Symposium "Selected topics on chromatin structure and function" held at the University of Camerino, Italy, at the end of May 1985. Times when the bacterial DNA was regarded as "naked" or, at most, complexed with polyamines, and when the absence of histones and organized chromatin was considered to be a distinct feature of the pro­ karyotic cell, now appear remote. Our concepts of how DNA is packaged in bacteria are changing rapidly. Studies on the structure of the bacterial nucleoid are not new. Recently, however, investigations in this field have flourished again, leading to some important contributions such as the elucidation of the three-dimensional structure of what appears to be the major protein constituent of the bacterial nucleoid or the development of methods to titrate the extent of DNA supercoiling within the bacterial cell.

• DNA
• bacteria
• biochemistry
• cell
• chemistry
• genetics
• protein

• Department of Cell Biology, Laboratory of Genetics, University of Camerino, Camerino (MC), Italy

  Claudio O. Gualerzi

• Max-Planck-Institut für Molekulare Genetik, Berlin 33, Germany

  Claudio O. Gualerzi, Cynthia L. Pon

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